Camera trap strapped to a tree trunk in a forest, one of 188 ecological survey methods

Ecological Survey Methods: Find the Right Field Survey Technique in 30 Seconds

Which survey method do I need?

Tell the tool what you are studying, what you need to know, where and when, and it picks the field method, the effort, the season, the kit and the mistake to avoid on day one. 188 methods across 14 groups.

Birds

Point counts

Stand at a fixed point and record every bird seen or heard, usually within 50 or 100 m bands. The workhorse of breeding bird monitoring because it needs one person and no kit.

Answers
Is it here?, How many?, Where does it occur across sites?, How is it spread across the area?
Effort
10 to 20 points per site, 5 or 10 minutes each, 2 to 3 visits, dawn to 4 hours after sunrise
Season
Mar, Apr, May, Jun, Jul (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None for observation. Access permission for private land.
How to run it
  1. Place points at least 200 m apart (250 m in open country) and mark them on GPS the day before.
  2. Start within 30 minutes of sunrise; stop by mid morning or when wind exceeds Beaufort 3 or it rains.
  3. Wait 1 minute after arriving, then record for the fixed time, noting distance band, detection type (seen, song, call) and flyovers separately.
  4. Repeat every point 2 or 3 times across the season in the same order reversed.
  5. Enter data the same day; unreadable field sheets are the main data loss.
Kit list

The day one mistake: Counting the same singing male twice from two points. Note direction and distance so duplicates can be removed.

Birds

Line transects

Walk a fixed line and record birds with their perpendicular distance from the line. Suited to open country where detections happen over long distances and you want density.

Answers
How many?, How is it spread across the area?
Effort
1 to 2 km lines walked at 1 to 2 km per hour, 2 visits, perpendicular distances recorded
Season
Mar, Apr, May, Jun, Jul (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None for observation.
How to run it
  1. Lay out straight lines that cross habitat gradients, not along tracks or edges.
  2. Walk at a steady pace, recording species, number, side, and perpendicular distance (estimate or rangefinder).
  3. Never leave the line to chase a bird; record where it was first detected.
  4. Two visits, early season and late season, same time of day.
  5. Keep distance bands consistent if you are not measuring exactly (0 to 25, 25 to 100, over 100 m).
Kit list

The day one mistake: Recording distance to where the bird flew, not where it was first seen. Distance sampling only works with first detection distances.

Birds

Territory mapping

Map every singing male and breeding behaviour on repeated visits, then cluster the registrations into territories. The most accurate small plot method and the most labour hungry.

Answers
How many?, What is it doing?, How is it spread across the area?
Effort
8 to 10 visits across the breeding season, whole plot walked each visit, 10 to 20 ha plots
Season
Mar, Apr, May, Jun, Jul (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None for observation.
How to run it
  1. Prepare a plot map at 1:2,500 with landmarks; number the visits.
  2. Walk within 50 m of every part of the plot each visit, alternating direction and start point.
  3. Plot each registration with species code and activity symbol (singing, alarm, nest material, food carrying, simultaneous singers).
  4. Transfer to species maps the same evening.
  5. After the final visit, draw territory clusters using at least 2 or 3 registrations per cluster.
Kit list

The day one mistake: Missing simultaneous singers. Two males heard at once are two territories; write the double headed arrow every time.

Birds

Nest searching and monitoring

Find nests by behaviour cues and check them on a fixed schedule to measure clutch size, hatching and fledging success.

Answers
What is it doing?, How many?
Effort
Visits every 3 to 4 days, under 1 minute at the nest, whole season
Season
Mar, Apr, May, Jun, Jul (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Often a permit. Disturbing protected species at the nest is an offence in most countries; check the schedule before you start.
How to run it
  1. Watch adults from a distance; nest material, food carrying and repeated flights to one spot lead you in.
  2. Approach only when no predator is watching, never in rain, and leave no dead end trail in vegetation.
  3. Record contents in under a minute; use a mirror pole for high nests.
  4. Return every 3 to 4 days; predict fledge date and check just before it.
  5. Record fate codes exactly as the protocol defines them; ambiguous fates get their own code.
Kit list

The day one mistake: Flagging the nest itself. Mark a point 10 m away with a bearing and distance, or predators will follow your tape.

Birds

Acoustic recorders (passive monitoring)

Leave autonomous recorders to capture dawn chorus or night calls, then identify species with software and manual checking. Scales to many sites with one person.

Answers
Is it here?, Where does it occur across sites?, How is it spread across the area?
Effort
1 recorder per site for 3 to 7 nights or dawns, 2 to 4 h per morning, batch analysed
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
None for recording. Landowner permission.
How to run it
  1. Set the schedule (for example 1 h before to 3 h after sunrise) and test one unit at home first.
  2. Mount at 1.5 m on a post or tree facing open habitat, away from running water and roads.
  3. Log GPS, deployment time, card ID and battery level for every unit.
  4. Retrieve, back up cards immediately, and label folders by site and date.
  5. Run automated ID (BirdNET or similar) then verify every new species by ear.
Kit list

The day one mistake: Trusting automated IDs. Confidence scores are not detections; check spectrograms before anything enters the dataset.

Birds

Mist netting and ringing

Catch birds in fine nets for ringing, measurement and survival estimates. Only ever under a licensed ringer.

Answers
Is it here?, How many?, What is it doing?
Effort
6 to 12 nets, opened at dawn for 5 to 6 h, checked every 20 to 30 minutes, constant effort sites repeat the same nets all season
Season
Apr, May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
Licence required everywhere (BTO permit in the UK, federal and state banding permits in the US). You may assist under a trainer's licence.
How to run it
  1. Set nets the evening before along flight lines and edges, furled.
  2. Open at first light; close in rain, strong wind or heat.
  3. Check every 20 to 30 minutes; extract feet first, bird held in ringer's grip.
  4. Ring, age, sex, wing, weight, fat, then release at the net.
  5. Record net hours exactly; effort is the denominator for every analysis.
Kit list
  • Mist nets
  • Net poles (Avinet (US) or NHBS (UK))
  • Bird bags (Avinet (US) or NHBS (UK))
  • Wing rule (Avinet (US) or NHBS (UK))
  • Spring balance (Pesola, via Forestry Suppliers (US) or NHBS (UK))

The day one mistake: Leaving a net unattended past 30 minutes. Bird welfare comes before data, and one dead bird ends most training relationships.

Birds

Nocturnal playback surveys (owls, nightjars, rails)

Broadcast the species' call at fixed points and record responses. The only practical way to survey secretive nocturnal birds.

Answers
Is it here?, Where does it occur across sites?
Effort
Points 500 m to 1 km apart, 2 to 3 visits, first 3 hours after dusk, calm nights; tawny owls also call in October and November
Season
Feb, Mar, Apr, May, Jun, Jul, Oct, Nov (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: no playback for Schedule 1 species (nightjar, barn owl and others) in the breeding season without a licence; the BTO nightjar survey forbids playback. US: prohibited on many refuges and parks. Check before use.
How to run it
  1. Load calls at a fixed volume and test the range in daylight.
  2. Listen 2 minutes silent, play 30 seconds, listen 2 minutes; repeat once.
  3. Record distance, direction and response type for each bird.
  4. Stop playback the moment a bird responds; do not draw it in.
  5. Three visits spread over the calling season.
Kit list

The day one mistake: Playing the call too long or too loud. It pulls birds off territory and biases every later point.

Bats

Roost emergence and re entry counts

Surveyors watch a building or tree and count bats leaving at dusk, with a detector to identify species. The standard for roost assessment.

Answers
Is it here?, How many?, What is it doing?
Effort
Start 15 minutes before sunset until 90 minutes after (or 90 minutes before sunrise for re entry), 2 to 3 surveys per roost, 2 to 4 surveyors
Season
May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Watching from outside needs no licence. Entering a roost or handling bats needs a licence (UK Natural England class licence; US state permits).
How to run it
  1. Inspect the structure by day for droppings, staining and gaps; assign one surveyor per emergence point.
  2. Position with the sky as a backdrop, 5 to 10 m back, detector on.
  3. Count every bat out and every bat in; note time, exit point and species from calls.
  4. Surveys spaced at least 2 weeks apart across May to September in the north.
  5. Log weather; abandon below 10 C or in rain.
  6. North America: follow the USFWS white nose syndrome decontamination protocol for every item that enters a roost or touches a bat.
Kit list

The day one mistake: Arriving at sunset. The first bats can leave 10 minutes before sunset on warm evenings and you will miss the count that matters.

Bats

Activity transects with a handheld detector

Walk a fixed route after sunset with a detector, recording passes and species. Shows where bats feed and commute across a site.

Answers
Is it here?, How is it spread across the area?, What is it doing?
Effort
1 to 3 km walked at 2 km per hour from sunset, stops of 3 to 5 minutes every 200 m, 1 visit per month
Season
Apr, May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None for detector surveys.
How to run it
  1. Design the route to cross every habitat and include linear features (hedges, water).
  2. Start at sunset; walk slowly with the detector recording continuously.
  3. At each stop, listen and note species, passes and behaviour (feeding buzzes, social calls).
  4. Record GPS tracklog so passes can be mapped.
  5. Analyse recordings with software and check species by hand.
Kit list

The day one mistake: Pointing the detector at the ground. Hold it at chest height, angled slightly up and away from you, or you record your own footsteps.

Bats

Static detectors (automated)

Leave recording detectors out for several nights per month to capture activity you would never hear on a walk. The standard for occupancy and seasonal patterns.

Answers
Is it here?, Where does it occur across sites?, How is it spread across the area?
Effort
1 detector per location; BCT 2023: 5 consecutive nights per month at moderate and high suitability sites, 5 nights per season at low suitability, sunset to sunrise
Season
Apr, May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
None for recording. Landowner permission.
How to run it
  1. Mount the microphone 1.5 to 2 m up, clear of vegetation, pointing across open space.
  2. Programme sunset to sunrise; check battery lasts 5 nights.
  3. Record GPS, deployment time and microphone direction.
  4. Retrieve; back up immediately; run automated ID then verify.
  5. Keep locations identical between months.
Kit list

The day one mistake: Placing the detector in dense vegetation. Clutter echoes bury the calls and you get thousands of unidentifiable files.

Bats

Harp trapping and mist netting

Capture bats on commuting routes for identification, measurement and tagging. Licensed work; you assist under a licence holder.

Answers
Is it here?, How many?, What is it doing?
Effort
Traps open sunset to 2 am, checked every 15 minutes, 2 to 4 nights per site
Season
May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Funded project
Licence
Licence required everywhere. Rabies vaccination usually required for handlers.
How to run it
  1. Set traps across flyways: rides, streams, gaps in hedges.
  2. Open at sunset; check every 15 minutes without fail.
  3. Process in a warm shelter: species, sex, age, forearm, weight, breeding status.
  4. Release at the capture point within 30 minutes.
  5. Record trap hours for effort.
  6. North America: follow the USFWS white nose syndrome decontamination protocol for every item that enters a roost or touches a bat.
Kit list
  • Harp trap (Austbat (AU) or NHBS (UK))
  • Mist nets
  • Callipers
  • Spring balance (Pesola, via Forestry Suppliers (US) or NHBS (UK))
  • Bat bags (Avinet (US) or NHBS (UK))

The day one mistake: Handling without gloves and without vaccination. Nobody will train you again after that.

Small mammals

Live trapping grids (Sherman or Longworth)

Set baited box traps in a grid, mark and release animals, and estimate abundance by mark recapture. The core small mammal method.

Answers
Is it here?, How many?, Where does it occur across sites?
Effort
49 to 100 traps on a 10 m grid, 3 to 5 consecutive nights, checked at dawn and dusk
Season
Mar, Apr, May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
Permits and ethics approval in most countries; shrews need a licence in the UK and traps must carry insect food and shrew provisions. Hantavirus precautions worldwide (Puumala and Dobrava in Europe, Sin Nombre in the Americas): gloves, no dust, traps cleaned outdoors.
How to run it
  1. Pre bait traps locked open for 2 nights so animals learn them.
  2. Set at dusk with bedding, seed and insect food (blowfly casters or mealworms) so shrews survive the night.
  3. Check at dawn and again at dusk, never later; where shrews are likely use escape holes or shorter intervals as the licence requires.
  4. Process: species, sex, weight, mark (fur clip or ear tag), trap position.
  5. Release at the trap. Record every trap as caught, empty, closed or missing.
  6. Close or remove traps on the last morning; count them all.
Kit list
  • Sherman traps (H.B. Sherman (US) or NHBS (UK))
  • Longworth traps (NHBS (UK); H.B. Sherman (US))
  • Pesola spring balance (Pesola, via Forestry Suppliers (US) or NHBS (UK))
  • Holding bags (Avinet (US) or NHBS (UK))
  • Headlamp

The day one mistake: Checking traps late. An animal in a trap for 12 hours in the sun is a dead animal and a failed ethics review.

Small mammals

Footprint tunnels and tracking plates

Baited tunnels with inked pads and paper record footprints of hedgehogs, small mammals and mustelids without handling.

Answers
Is it here?, Where does it occur across sites?
Effort
10 tunnels per site (1 km2 for hedgehogs), checked and re baited every day for 5 consecutive nights
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None.
How to run it
  1. Place tunnels along hedges and edges, 50 m apart, entrances flush with the ground.
  2. Ink the pads, insert fresh paper, bait the middle.
  3. Check every morning for 5 nights; replace paper and bait and photograph every sheet.
  4. Identify prints with a key; keep unidentified sheets.
  5. Fifty tunnel nights (10 tunnels for 5 nights) before recording absence.
Kit list
  • Footprint tunnels (NHBS (UK) or make from corrugated plastic)
  • Tracking ink (NHBS (UK) or Forestry Suppliers (US))
  • Cat food bait

The day one mistake: Too little ink or too much. Prints should be crisp; test on your own hand.

Small mammals

Hair tubes and hair traps

Baited tubes with sticky pads collect hair for identification of dormice, squirrels and martens.

Answers
Is it here?
Effort
20 to 30 tubes per site, 2 to 4 weeks
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None.
How to run it
  1. Attach tubes to branches or place on the ground along runs.
  2. Bait and fit fresh sticky pads.
  3. Collect pads after 2 to 4 weeks into labelled bags.
  4. Identify hair under a microscope or send for DNA.
  5. Record every tube, including empty ones.
Kit list

The day one mistake: Not labelling pads at collection. Twenty identical pads in a bag are worth nothing.

Small mammals

Owl pellet analysis

Dissect barn owl or other pellets to recover skulls and jaws, giving a cheap picture of small mammal communities across an area.

Answers
Is it here?, How is it spread across the area?
Effort
20 to 50 pellets per roost, collected quarterly
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Access to roosts may need permission; disturbing nesting owls needs a licence.
How to run it
  1. Collect pellets from known roosts and bag by site and date.
  2. Soak in water, tease apart, recover skulls, jaws and pelvic bones.
  3. Identify with a key; count minimum number of individuals per species.
  4. Record pellet count per site as effort.
  5. Store bones dry and labelled.
Kit list

The day one mistake: Counting jaws without pairing them. Use the higher of left or right counts per species.

Medium and large mammals

Camera trapping

Motion triggered cameras record mammals day and night. Occupancy from detection histories, abundance from spatial capture recapture for marked species, activity patterns from timestamps.

Answers
Is it here?, Where does it occur across sites?, How is it spread across the area?, What is it doing?, How many?
Effort
20 to 60 cameras, 1 per 1 to 4 km2, 30 to 60 days, checked every 2 to 4 weeks
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
Landowner permission; protected area permits. No licence for the cameras themselves.
How to run it
  1. Design the grid in GIS first; random or systematic points, then micro site within 50 m on a trail.
  2. Mount at 30 to 50 cm, angled slightly down, facing along the trail (north or south), 3 to 5 m from it.
  3. Set date and time, high sensitivity, 3 shot burst, 0 to 1 second delay (a 10 second delay misses the second animal in a pair); walk test and photograph a site card.
  4. Log GPS, camera ID, card ID, height, angle and vegetation clearance.
  5. Rename and tag images by site and date; record every camera day as effort, including failures.
Kit list

The day one mistake: Not photographing a whiteboard with site and date at deployment. Months later you will not know which card came from where.

Medium and large mammals

Sign surveys: tracks, scat and trails

Walk transects recording tracks, scat, digging and trails. Cheap, fast, and fine for presence and relative abundance indices.

Answers
Is it here?, How is it spread across the area?, How many?
Effort
1 to 2 km transects, 5 to 10 per site, walked once per season
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None. Some scat exports need permits.
How to run it
  1. Walk after rain or fresh snow when substrate is best.
  2. Record every sign with species, age class (fresh, old), substrate and GPS.
  3. Photograph tracks with a scale bar; measure length and width.
  4. Collect scat in labelled bags if DNA or diet analysis is planned.
  5. Keep transect length and walking speed constant for indices.
Kit list

The day one mistake: Recording sign of uncertain species as a species. Use a confidence code; certain, probable, possible.

Medium and large mammals

Distance sampling line transects (deer, antelope, primates)

Walk or drive straight lines and record the perpendicular distance to every group seen. Density is estimated from how detection drops with distance.

Answers
How many?, How is it spread across the area?
Effort
Total transect length of 40 km or more per site, 60 to 80 detections minimum, dawn and dusk
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None for observation.
How to run it
  1. Place lines randomly or systematically with a random start; never along roads.
  2. Walk at 1 km per hour in silence, scanning ahead.
  3. On detection: species, cluster size, sighting angle and radial distance (rangefinder and compass), convert to perpendicular later.
  4. Do not count animals flushed behind you.
  5. Repeat lines until you have at least 60 detections.
Kit list

The day one mistake: Guessing distance. The whole model rests on the first 50 m being measured accurately; use the rangefinder every time.

Medium and large mammals

Spotlight counts

Drive fixed routes at night with a spotlight and count eyeshine. Standard for hares, deer, foxes and nocturnal marsupials.

Answers
How many?, Is it here?
Effort
10 to 20 km driven at 15 km per hour, 2 observers, 3 repeats, starting 1 h after dark
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Landowner permission; some regions restrict spotlighting by law. Check before you start.
How to run it
  1. Fix the route and the side each observer scans.
  2. Sweep the light slowly in an arc; stop to identify with binoculars.
  3. Record species, number, distance and side.
  4. Same route, same speed, same season each year.
  5. Do not survey in rain, fog or bright moon without noting it.
Kit list

The day one mistake: Counting eyeshine as one species. Two eyes can be a rabbit, a cat or a nightjar; confirm every record.

Medium and large mammals

Pellet group counts (deer, hares, lagomorphs)

Count dung pellet groups in plots to index or estimate deer and lagomorph density from defecation and decay rates.

Answers
How many?
Effort
50 to 100 plots or 20 transects of 100 m x 2 m per site, cleared then re counted after 30 to 60 days
Season
Jan, Feb, Mar, Apr, Oct, Nov, Dec (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None.
How to run it
  1. Lay out plots along transects; clear all pellets on the first visit or record them as standing crop.
  2. Return after a fixed interval and count new groups (6 or more pellets, same age).
  3. Record decay state and vegetation.
  4. Use published defecation rates for the species and season, or measure your own.
  5. Walk slowly; searchers miss half the groups when rushed.
Kit list

The day one mistake: Not clearing plots on visit one. Standing crop counts need decay rates you do not have.

Medium and large mammals

Burrow, sett and den surveys

Systematic search for badger setts, fox earths, rabbit warrens and burrow systems, classified by activity.

Answers
Is it here?, How many?, How is it spread across the area?
Effort
Whole site walked in strips 20 m apart, once, when vegetation is low
Season
Feb, Mar, Apr, Oct, Nov (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Badger setts are legally protected in the UK; do not disturb entrances.
How to run it
  1. Walk parallel strips so every part of the site is within 10 m of your path.
  2. Record every entrance: GPS, size, spoil, bedding, hair, prints, activity level.
  3. Group entrances into setts or warrens; classify main, annexe, subsidiary, outlier.
  4. Photograph and sketch each system.
  5. Repeat in the other season if activity is uncertain.
Kit list

The day one mistake: Surveying in July. Bracken and nettles hide half the entrances; wait for the vegetation to die back.

Medium and large mammals

GPS collaring and telemetry

Capture and fit GPS collars to track movement, home range and habitat selection. Funded project work; you will assist a vet and a licensed team.

Answers
What is it doing?, How is it spread across the area?
Effort
5 to 20 collared animals, fixes every 1 to 4 hours, 6 to 24 months
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Funded project
Licence
Animal capture and marking permits, ethics approval and a vet in every jurisdiction.
How to run it
  1. Capture protocol is written by the vet and licence holder; learn your role before the day.
  2. Collar fit: two fingers under the belt; record weight, collar ID, frequency.
  3. Download or receive fixes on schedule; screen for errors (DOP, impossible speeds).
  4. Ground truth a sample of fixes with the handheld receiver.
  5. Plan collar removal before deployment.
Kit list
  • VHF receiver and antenna (Lotek, ATS or Biotrack)
  • Handheld GPS
  • Collar and drop off unit (Lotek, Vectronic or ATS)

The day one mistake: Trusting every fix. Filter by DOP and speed before any analysis.

Amphibians

Pond surveys: torch counts and netting

Walk the pond edge at night with a torch counting newts and frogs, then net the margins. Combined with egg searching and bottle traps this is the standard newt method.

Answers
Is it here?, How many?
Effort
4 visits per pond (presence) or 6 (population size) between mid March and mid June, at least 2 between mid April and mid May, from 1 h after dark; torch the whole margin, then net about 15 minutes per 50 m of shoreline
Season
Mar, Apr, May, Jun (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Great crested newt (UK): every standard method except eDNA, including torching, netting, bottle trapping and egg searching, needs a survey licence from Natural England, NRW or NatureScot. Elsewhere: state or national permits for netting and handling.
How to run it
  1. Visit in calm, mild conditions (over 5 C), after dark; use at least 3 of the 4 methods (torch, net, bottle trap, egg search) on every visit.
  2. Torch first: walk the whole margin slowly with one beam through the water, counting by species and sex.
  3. Net after torching, about 15 minutes per 50 m of shoreline, and identify catches in a tray.
  4. Search submerged leaves for folded eggs; stop unfolding leaves the moment the first great crested newt egg is confirmed, because unfolding kills them.
  5. Record pond conditions: turbidity, weed cover, shading, fish, and the Habitat Suitability Index.
  6. Disinfect nets, waders, trays and traps between sites (Virkon S at 1 percent for 1 minute) and dry fully; chytrid and ranavirus travel on wet kit.
Kit list

The day one mistake: Torching in a group. Three lights make three sets of shadows and the newts vanish; one torch, one counter.

Amphibians

Egg mass counts

Count frog and salamander egg masses in a pond to estimate the breeding female population without handling adults.

Answers
How many?
Effort
2 to 3 visits per pond in the laying season, whole pond searched, day time
Season
Feb, Mar, Apr (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Usually none for counting; handling may need a licence.
How to run it
  1. Survey in daylight when masses are freshly laid and before they disperse.
  2. Walk the margin and wade shallow water, counting masses by species.
  3. Mark counted masses on a sketch map or with small flags to avoid double counts.
  4. Record depth, attachment and development stage.
  5. Repeat a week later; use the maximum count.
  6. Disinfect nets, waders, trays and traps between sites (Virkon S at 1 percent for 1 minute) and dry fully; chytrid and ranavirus travel on wet kit.
Kit list

The day one mistake: Counting after a warm week. Masses hatch and disappear fast; time the visit to the local laying peak.

Amphibians

Bottle and funnel trapping

Funnel traps set overnight along pond margins catch newts and larvae that torching misses.

Answers
Is it here?, How many?
Effort
1 trap per 2 m of margin, set at dusk, checked within 12 h, 4 to 6 nights
Season
Mar, Apr, May, Jun (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Licence required for protected species in most places.
How to run it
  1. Set traps at dusk, angled so the top is above water for air.
  2. Do not trap when the overnight air temperature is forecast below 5 C or in warm, weed choked, deoxygenated water; set at dusk and lift by mid morning.
  3. One trap per 2 m of shoreline; mark positions.
  4. Check at first light; never leave traps out in the day.
  5. Process in a tray: species, sex, stage; release at the trap.
  6. Disinfect all kit between ponds.
Kit list

The day one mistake: Sinking the trap fully. Newts drown; leave an air pocket every time.

Amphibians

Call surveys (frogs and toads)

Listen at fixed points and score calling intensity by species. A cheap way to cover many wetlands in a season.

Answers
Is it here?, Where does it occur across sites?
Effort
3 visits per site, 5 minute listening periods, from 30 minutes after sunset, warm humid nights
Season
Mar, Apr, May, Jun, Jul (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None. The 0 to 3 call index is the NAAMP standard (US and Canada); other schemes use their own scale.
How to run it
  1. Wait 1 minute after arrival, then listen for 5 minutes.
  2. Score each species 0 to 3 (none, individuals countable, overlapping, full chorus).
  3. Record air temperature, Beaufort wind, sky code, noise index and moon; finish by 01:00.
  4. Visit early, mid and late season because species peak at different times.
  5. Record the recording as well; software can check your ears.
Kit list

The day one mistake: Listening for 30 seconds. Late calling species need the full 5 minutes.

Amphibians

Environmental DNA sampling

Collect water samples and test for species DNA in a lab. Detects secretive species with one daytime visit.

Answers
Is it here?, Where does it occur across sites?
Effort
20 subsamples pooled per pond, 1 visit in the breeding season, lab analysis
Season
Apr, May, Jun (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
None for water sampling; lab and species may have accreditation requirements (UK great crested newt).
How to run it
  1. Order kits and follow the kit protocol exactly; wear fresh gloves per pond.
  2. Take 20 subsamples around the whole margin into the sterile bag.
  3. Mix and fill the preservative tubes; label and chill.
  4. Record pond conditions and the time since any rain.
  5. Ship within the kit's time limit.
Kit list

The day one mistake: Contaminating the sample with your own kit. New gloves, no reused bags, and never dip the net first.

Amphibians

Drift fences with pitfalls

A low fence directs migrating amphibians into buckets, counting every animal entering or leaving a pond.

Answers
How many?, How is it spread across the area?, What is it doing?
Effort
Fence encircling the pond or 50 m arrays, buckets every 5 to 10 m, checked every morning, 4 to 8 weeks
Season
Feb, Mar, Apr, May, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
Permits and ethics approval; licence for protected species.
How to run it
  1. Dig the fence in 10 cm, buckets flush with the ground on both sides.
  2. Add a sponge and cover to each bucket; check every morning without fail.
  3. Process: species, sex, stage, direction; release on the far side.
  4. Record bucket nights as effort.
  5. Remove or close buckets at the end; never leave them open.
  6. Disinfect nets, waders, trays and traps between sites (Virkon S at 1 percent for 1 minute) and dry fully; chytrid and ranavirus travel on wet kit.
Kit list

The day one mistake: Missing a morning check. A hot bucket kills everything in it.

Reptiles

Refugia (tin and felt) surveys

Lay sheets of tin or roofing felt that reptiles bask under, then lift them on repeated visits. The standard for snakes and lizards in temperate grassland.

Answers
Is it here?, How many?
Effort
20 to 50 refugia per hectare, left 2 weeks before checking, 7 visits for presence or absence and 20 for population assessment, mornings at 9 to 18 C
Season
Apr, May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: sand lizard and smooth snake sites need a licence to lift refugia; common species need none. Elsewhere: handling protected species needs a permit.
How to run it
  1. Place refugia on south facing edges, half in sun, in sheltered spots; GPS every one.
  2. Leave for 2 weeks before the first check.
  3. Check between 9 and 11 am on mild days at 9 to 18 C; lift slowly from the far side.
  4. Record species, number, age, and whether on top or under.
  5. Twenty checks before you record absence.
  6. Disinfect nets, waders, trays and traps between sites (Virkon S at 1 percent for 1 minute) and dry fully; chytrid and ranavirus travel on wet kit.
Kit list

The day one mistake: Checking at noon in July. Reptiles have already warmed up and left; go early or on cool bright days.

Reptiles

Visual encounter transects

Walk slowly along edges and basking sites, recording every reptile seen. Effort is time, so results are comparable between sites.

Answers
Is it here?, How is it spread across the area?
Effort
Transects of 30 to 60 minutes timed search, 3 to 5 repeats, mornings
Season
Mar, Apr, May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None for observation.
How to run it
  1. Walk at 1 km per hour scanning 2 m ahead for basking animals.
  2. Record species, GPS, substrate and behaviour.
  3. Note time searched per section.
  4. Repeat at the same time of day.
  5. Include night walks for nocturnal geckos in warm climates.
Kit list

The day one mistake: Walking too fast. Most lizards are seen because they move when you are 2 m away; give them the chance.

Reptiles

Drift fences with pitfall and funnel traps

Fences funnel moving reptiles into buckets and funnel traps. The main abundance method in arid and tropical systems.

Answers
How many?, How is it spread across the area?
Effort
Y shaped arrays with 3 fences of 15 m, buckets and funnels at the ends, checked every morning, 2 to 4 weeks
Season
Mar, Apr, May, Jun, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
Permits and ethics approval in most places; venomous species need training.
How to run it
  1. Build Y arrays with buckets at the centre and ends, funnel traps along each arm.
  2. Shade every trap and add a moist sponge.
  3. Check every morning; process and release 20 m away on the far side.
  4. Record trap nights.
  5. Close traps when not in use.
  6. Disinfect nets, waders, trays and traps between sites (Virkon S at 1 percent for 1 minute) and dry fully; chytrid and ranavirus travel on wet kit.
Kit list

The day one mistake: Reaching into a bucket without looking. Tip it toward you and look first, always.

Freshwater invertebrates and plankton

Kick sampling for macroinvertebrates

Disturb the river bed upstream of a net for a timed period and sort what you catch. The basis of biotic water quality indices.

Answers
Is it here?, How many?
Effort
3 minute kick plus 1 minute hand search per site, 1 to 2 visits per season
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Some countries require a permit to sample protected rivers; disinfect kit between catchments.
How to run it
  1. Sample all habitats in proportion: riffles, margins, weed, silt.
  2. Kick for 3 minutes total, moving upstream, net downstream of your feet.
  3. One minute hand search of stones and vegetation.
  4. Sort live in a tray or preserve in 70 percent ethanol.
  5. Identify to family (or species where the index needs it) and record abundance classes.
Kit list

The day one mistake: Sampling only the riffle. Half the families live in margins and weed.

Fish

Electrofishing (assisting)

A licensed operator stuns fish with an electric field while assistants net, hold and process them. You will assist, never lead, until trained.

Answers
How many?, Is it here?
Effort
3 pass depletion over a 50 to 100 m stop netted reach, team of 3 to 4
Season
Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Funded project
Licence
Licence, permit and certified training required everywhere. Insulated waders and gloves mandatory.
How to run it
  1. Stop net the reach top and bottom.
  2. Walk upstream in a line; net every stunned fish quickly into a bucket with aeration.
  3. Three passes; keep passes separate.
  4. Measure, weigh, identify and release below the reach after recovery.
  5. Record settings, time fished and conductivity.
Kit list

The day one mistake: Touching the water without the anode off. Say 'off' and wait for the reply every time.

Fish

Fyke, minnow and crayfish traps

Baited or unbaited traps left overnight catch fish, crayfish and amphibian larvae for presence and relative abundance.

Answers
Is it here?, How many?
Effort
5 to 10 traps per site, set overnight, 3 nights
Season
Apr, May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Trapping licence from the fisheries authority in most countries; otter guards are legally required on fyke nets in the UK.
How to run it
  1. Set with the top above water or an air space (otter guards on fykes).
  2. Anchor and mark; GPS.
  3. Check at first light; process and release.
  4. Record trap nights.
  5. Disinfect between waters; invasive crayfish must not be moved.
  6. Disinfect nets, waders, trays and traps between sites (Virkon S at 1 percent for 1 minute) and dry fully; chytrid and ranavirus travel on wet kit.
Kit list

The day one mistake: Forgetting the otter guard. A drowned otter is a criminal offence and the end of the project.

Fish

Snorkel surveys

Snorkel upstream counting fish by species and size class. Non lethal, fast and good for clear water and spawning behaviour.

Answers
How many?, What is it doing?
Effort
50 to 100 m reaches, 1 to 3 snorkelers moving upstream, 2 passes
Season
Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None usually; protected area permits.
How to run it
  1. Only survey clear water over 30 cm deep; measure visibility with a marked pole.
  2. Move upstream slowly, lanes agreed between snorkelers.
  3. Count by species and size class; call to a bank recorder.
  4. Two passes 30 minutes apart.
  5. Never survey alone.
Kit list

The day one mistake: Surveying alone in cold water. Hypothermia and entrapment happen fast.

Freshwater invertebrates and plankton

Zooplankton and plankton sampling

Tow a fine mesh net through the water column and identify and count what you catch under a microscope.

Answers
Is it here?, How many?, How is it spread across the area?
Effort
Vertical or horizontal net tows per station, 3 replicates, monthly
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None.
How to run it
  1. Record station GPS, depth and Secchi depth.
  2. Lower the net to depth and haul at 0.5 m per second; note the volume filtered.
  3. Rinse the cod end into a labelled pot; preserve in 4 percent formalin or ethanol.
  4. Subsample with a Stempel pipette; count and identify in a counting chamber.
  5. Scale counts to individuals per litre.
Kit list

The day one mistake: Not recording the volume filtered. A count without volume is not a density.

Terrestrial invertebrates and pollinators

Pitfall trapping

Cups sunk flush with the ground catch ground beetles, spiders and ants. The standard for ground active invertebrates.

Answers
How many?, Is it here?
Effort
10 traps per site, 10 m apart, 7 to 14 days open, 2 to 4 rounds
Season
Apr, May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: unroofed pitfalls drown shrews (Schedule 6), so fit vertebrate exclusion and operate under the Natural England shrew general licence; protected sites need consent. Elsewhere: check protected invertebrate lists.
How to run it
  1. Sink cups flush with the soil; use a sleeve so you can lift the inner cup.
  2. Add preservative (propylene glycol) and a rain cover.
  3. Fit a 10 mm mesh or a raised roof with a narrow gap so shrews, frogs and lizards cannot fall in.
  4. Empty every 7 to 14 days into labelled pots.
  5. Sort and identify in the lab; record trap days.
  6. Remove cups at the end of the study.
Kit list

The day one mistake: Leaving a lip above the soil. Beetles walk around it and your catch halves.

Terrestrial invertebrates and pollinators

Sweep netting

Sweep a net through vegetation a set number of times and count the catch. Fast and cheap for grassland insects.

Answers
Is it here?, How many?
Effort
20 sweeps per sample, 5 samples per site, dry warm days, monthly
Season
May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None.
How to run it
  1. Sweep in a figure of eight, net mouth vertical, 20 sweeps at a steady pace.
  2. Empty into a bag or tray; record only when vegetation is dry.
  3. Identify to order or family in the field; preserve specimens you cannot name.
  4. Note vegetation height and flowering.
  5. Same time of day each visit.
Kit list
  • Sweep net (Watkins and Doncaster (UK) or Forestry Suppliers (US))
  • Pooter (Watkins and Doncaster (UK) or Forestry Suppliers (US))
  • Specimen pots

The day one mistake: Sweeping wet grass. Everything sticks to the net and the sample is useless.

Terrestrial invertebrates and pollinators

Malaise traps

A tent like trap intercepts flying insects and funnels them into a collecting bottle. Huge samples, ideal for DNA metabarcoding.

Answers
How many?, How is it spread across the area?
Effort
1 to 2 traps per site, emptied weekly, whole season
Season
Apr, May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
None usually.
How to run it
  1. Site across a flight line, collecting head at the highest and sunniest end.
  2. Fill the bottle with 80 percent ethanol.
  3. Change the bottle weekly; label with site and dates.
  4. Record days deployed.
  5. Sort or send for metabarcoding.
Kit list

The day one mistake: Facing the head into the shade. Insects fly toward light; orient the head correctly or catch nothing.

Terrestrial invertebrates and pollinators

Light trapping (moths)

A mercury vapour or actinic light attracts moths into a box overnight. Counted and released in the morning.

Answers
Is it here?, How many?
Effort
1 trap per site, dusk to dawn, weekly or fortnightly, warm cloudy nights
Season
Mar, Apr, May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
UK: Schedule 5 moths may not be taken and protected sites need consent for traps. US: some state permits near protected species habitat.
How to run it
  1. Set on a sheet in a sheltered spot; power from mains, generator or battery.
  2. Run dusk to dawn; record min temperature and cloud.
  3. In the morning, identify and count every moth, then release into cover.
  4. Photograph anything unknown.
  5. Same site, same trap, same schedule.
Kit list
  • Actinic moth trap (Anglian Lepidopterist Supplies (UK) or Forestry Suppliers (US))
  • Egg boxes
  • Moth field guide (NHBS (UK) or a bookshop (US))

The day one mistake: Releasing at the trap in daylight. Birds learn the buffet; release into dense cover away from the trap.

Terrestrial invertebrates and pollinators

Pollard walks (butterflies and dragonflies)

Walk a fixed route weekly recording every butterfly within a 5 m box ahead. The method behind national butterfly monitoring schemes.

Answers
How many?, How is it spread across the area?
Effort
Fixed route of 2 to 4 km in up to 15 sections, walked weekly at a steady pace from 1 April to the end of September (26 weeks), between 10:45 and 15:45
Season
Apr, May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None.
How to run it
  1. Divide the route into habitat sections.
  2. Walk at a steady pace recording butterflies within 2.5 m either side and 5 m ahead.
  3. Weather rule (UKBMS): at least 13 C with 60 percent sunshine, or at least 17 C in any conditions if dry; wind below Beaufort 5. North American schemes (NABA, Ohio) use their own thresholds.
  4. Weekly for the whole season; record start and end time, temperature, sun and wind.
  5. Enter data into the national scheme if one exists.
Kit list

The day one mistake: Chasing a butterfly out of the box. Record it where you first saw it and keep walking.

Terrestrial invertebrates and pollinators

Pan traps (pollinators)

Coloured bowls of soapy water attract bees and hoverflies. Standardised and cheap for pollinator surveys.

Answers
Is it here?, How many?
Effort
Triplets of yellow, white and blue bowls at 5 stations, 6 hours on a sunny day, monthly
Season
Apr, May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None.
How to run it
  1. Set bowl triplets at vegetation height, 15 m apart.
  2. Fill with water and a drop of unscented soap.
  3. Leave 6 hours in sun; collect into ethanol.
  4. Record flowering cover around each station.
  5. Pin or send for identification.
Kit list

The day one mistake: Setting bowls on the ground in tall grass. Raise them to flower height or they are invisible.

Plants and vegetation

Quadrat sampling

Record species and cover inside a frame. The basic unit of vegetation description.

Answers
How many?, Is it here?
Effort
10 to 30 quadrats of 1 m x 1 m (grassland) or 2 m x 2 m (heath), random or along transects
Season
May, Jun, Jul, Aug (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None; permission for protected sites and plants.
How to run it
  1. Choose quadrat size by vegetation: 0.25 m2 for short turf, 1 m2 grassland, 4 m2 heath, 100 m2 woodland canopy.
  2. Place randomly (random numbers along a tape) or at fixed intervals on a transect.
  3. List every species; estimate percent cover or use a scale (Domin or Braun Blanquet).
  4. Record bare ground, litter and vegetation height.
  5. Photograph each quadrat from above with its number.
Kit list
  • Quadrat frame 1 m (NHBS (UK), Forestry Suppliers (US), or make from PVC pipe)
  • Measuring tape 30 m
  • Plant field guide (NHBS (UK) or a bookshop (US))
  • Hand lens

The day one mistake: Estimating cover before listing species. List first, then estimate, or you will miss the small ones.

Plants and vegetation

Line and belt transects

Lay a tape across a gradient (dune, shore, slope) and record species along it. Shows how vegetation changes with environment.

Answers
How is it spread across the area?, How many?
Effort
Transects across the gradient, 20 to 100 m, quadrats or point records every 1 to 5 m
Season
May, Jun, Jul, Aug (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None.
How to run it
  1. Run the tape from the start of the gradient to beyond its end.
  2. Record species touching the tape at set intervals (line) or in quadrats along it (belt).
  3. Record height, slope and soil moisture at each station.
  4. Mark the ends for repeat visits.
  5. Draw the profile.
Kit list

The day one mistake: Running the transect along the gradient instead of across it. Nothing will change along the tape.

Plants and vegetation

Relevés and community classification plots

Full species lists with cover in homogenous stands, used to classify vegetation communities.

Answers
Is it here?, How is it spread across the area?
Effort
5 or more plots per community, 4 m2 to 400 m2 depending on structure
Season
May, Jun, Jul, Aug (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None; permits for collecting specimens on protected sites.
How to run it
  1. Pick a homogeneous stand; place the plot away from edges.
  2. Record every vascular plant and bryophyte with a cover score.
  3. Record layers separately in woodland: canopy, shrub, field, ground.
  4. Note aspect, slope, soil and management.
  5. Match to a classification key later.
Kit list

The day one mistake: Placing the plot where the flowers are. It must represent the stand, not the prettiest corner.

Plants and vegetation

Habitat mapping and walkover surveys

Walk the site mapping habitat types and dominant species with target notes. The first survey on almost every consulting project.

Answers
Is it here?, How is it spread across the area?
Effort
Whole site walked at 1 to 2 ha per hour, 1 visit in the growing season
Season
Apr, May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None; landowner access.
How to run it
  1. Print aerial imagery at 1:2,500 and pre draw likely boundaries.
  2. Walk every parcel; classify habitat by the local scheme; record dominant species with a DAFOR scale.
  3. Write target notes for features (ponds, veteran trees, invasive species).
  4. GPS boundaries where the imagery is unclear.
  5. Digitise in GIS the same week.
Kit list

The day one mistake: Mapping from the path. Walk into each parcel; the far corner is where the rare thing is.

Plants and vegetation

Point intercept

Lower a pin at fixed points and record every species it touches. Objective cover data that repeats well between observers.

Answers
How many?
Effort
100 to 200 points per transect, pins every 0.5 or 1 m, 3 transects per site
Season
May, Jun, Jul, Aug (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None.
How to run it
  1. Lay a tape; at each interval lower the pin vertically.
  2. Record every species touched, top to bottom, and the ground surface hit.
  3. Cover equals hits divided by points.
  4. Keep the pin diameter and drop angle fixed.
  5. Repeat the same tape positions each year.
Kit list

The day one mistake: Recording only the first hit. Multiple hits per pin are the point of the method.

Plants and vegetation

Permanent plots and tree monitoring

Tag and measure every tree in a fixed plot to track growth, mortality and recruitment.

Answers
What is it doing?, How is it spread across the area?, How many?
Effort
Plots of 20 m x 20 m or larger, all stems over 5 cm DBH tagged, remeasured every 1 to 5 years
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
Permission; some reserves need research permits.
How to run it
  1. Mark corners with permanent posts; GPS and bearings.
  2. Tag every stem over the threshold; measure DBH at 1.3 m (UK, Europe, RAINFOR and ForestGEO) or 1.37 m (US Forest Inventory), and record which.
  3. Map stems by coordinates within the plot.
  4. Record status codes (alive, dead standing, fallen).
  5. Remeasure at the same season.
Kit list
  • Diameter tape (Forestry Suppliers (US) or NHBS (UK))
  • Tree tags and nails (Forestry Suppliers (US))
  • Clinometer

The day one mistake: Measuring DBH at different heights. Paint the 1.3 m line on the trunk.

Marine benthos, reefs and shore

Intertidal belt transects and quadrats

Work down the shore on the lowest tides recording species in quadrats at fixed heights. The core rocky shore method.

Answers
How many?, How is it spread across the area?
Effort
Transects from high to low shore on spring low tides, quadrats every 1 to 2 m vertical height, 2 hours either side of low water
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None; protected areas need permits.
How to run it
  1. Check tide tables; start 2 hours before low water.
  2. Run the transect from the strandline to the water's edge; measure height with a cross staff or level.
  3. Place quadrats at fixed heights; count animals, estimate algae cover.
  4. Photograph each quadrat.
  5. Leave before the tide turns.
Kit list

The day one mistake: Ignoring the tide. People drown on rocky shores every year; someone on shore knows your plan.

Marine benthos, reefs and shore

Underwater visual census (reef fish)

Divers swim a tape counting fish by species and size within a belt. The reef monitoring standard.

Answers
How many?, What is it doing?
Effort
Belt transects of 50 m x 5 m, 4 to 6 per site, 2 divers, fixed depth
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Funded project
Licence
Dive certification; research and marine park permits.
How to run it
  1. Lay the tape; wait 3 minutes for fish to settle.
  2. Swim at 5 m per minute counting fish within 2.5 m either side and 5 m above.
  3. Record species, number and size class on a slate.
  4. Second diver records benthos or repeats the count.
  5. Fixed depth and time of day.
Kit list

The day one mistake: Counting fish that follow you. Record only what is ahead of the leading diver.

Marine mammals and seabirds

Boat based cetacean and seabird line transects

Observers scan from a vessel along pre planned lines recording every sighting with angle and distance. Distance sampling at sea.

Answers
How many?, How is it spread across the area?
Effort
Systematic zig zag lines at 10 knots, 2 observers plus recorder, sea state 3 or less (2 or less for harbour porpoise)
Season
Apr, May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Funded project
Licence
Vessel and marine park permits; no licence for observation.
How to run it
  1. Plan lines with random start; log effort continuously (on, off, sea state).
  2. Two observers scan 90 degrees each with naked eye, binoculars to confirm.
  3. Cetaceans: on sighting record time, species, group size, angle (angle board) and distance (reticle binoculars).
  4. Seabirds (ESAS method): count a 300 m strip on one side in distance bands A to D (0 to 50, 50 to 100, 100 to 200, 200 to 300 m) for birds on the water, and snapshot counts for birds in flight.
  5. Recorder logs GPS every minute; stop effort above the sea state limit.
Kit list
  • Reticle binoculars 7×50 (Steiner or Fujinon, via an optics dealer)
  • Angle board (make from a protractor and a board)
  • Handheld GPS

The day one mistake: Chasing the animals. Stay on the line; the model assumes you did not move.

Marine mammals and seabirds

Seabird colony counts

Count apparently occupied nests or sites from vantage points or photographs during the incubation window set for each species.

Answers
How many?, What is it doing?
Effort
Whole colony counts 2 to 3 times, or study plots for guillemot and razorbill counted 5 to 10 times (mean), inside the species' standard window (auks 08:00 to 16:00, first three weeks of June in the UK; puffin burrows in April to early May before vegetation grows)
Season
Apr, May, Jun, Jul (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Colonies are usually protected; landing may need a permit.
How to run it
  1. Define count units by species: apparently occupied nests for kittiwakes and gulls, individuals on ledges for guillemots and razorbills, apparently occupied burrows for puffins.
  2. Count from fixed vantage points on 2 to 3 days inside the standard date and time window for the species.
  3. Use photographs for dense cliffs and count on screen.
  4. Record disturbance and weather; do not count in rain or strong wind.
  5. Use the mean of counts for auks and the maximum for nests.
Kit list

The day one mistake: Counting outside the species' date and time window. Attendance swings by the hour and the week, and counts from different windows cannot be compared.

Marine mammals and seabirds

Shore counts and strandings surveys

Walk fixed beach sections recording stranded animals, seals hauled out and shore birds. Cheap, repeatable and shareable with citizen scientists.

Answers
Is it here?, How is it spread across the area?
Effort
Fixed beach sections walked monthly at low tide
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Handling carcasses of protected species may need a permit; report to the national strandings scheme.
How to run it
  1. Walk the same section at the same tide state each month.
  2. Record every carcass: species, length, decomposition, GPS, photos.
  3. Count seals from a distance; note age classes.
  4. Report strandings to the national scheme.
  5. Never handle carcasses without gloves.
Kit list

The day one mistake: Not photographing the whole animal with a scale. The scheme cannot verify a species from a head shot.

Fish

Environmental DNA for fish

Filter water and test for fish DNA in the lab. Detects rare and invasive fish without nets or electricity.

Answers
Is it here?, Where does it occur across sites?
Effort
3 replicate water samples per site, filtered on site, 1 visit per season
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
None for sampling; some waters need access consent.
How to run it
  1. Sample upstream of where you stand; fresh gloves per site.
  2. Filter 1 to 2 L through the kit's filter until it clogs; record the volume.
  3. Preserve the filter as the kit instructs; label and chill.
  4. Take a field blank at every site.
  5. Record flow, temperature and turbidity.
Kit list

The day one mistake: Skipping the field blank. Without it a positive result cannot be defended.

Fungi, lichens and bryophytes

Fixed route fungal forays

Walk the same route repeatedly recording every fruiting body. Fungi fruit unpredictably, so repetition is the method.

Answers
Is it here?, How is it spread across the area?
Effort
Fixed route of 1 to 2 km walked every 2 weeks through the fruiting season, 2 to 3 seasons before a list is meaningful
Season
Aug, Sep, Oct, Nov (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Collecting may need permission on reserves; some countries regulate picking.
How to run it
  1. Map the route through every habitat and substrate type (soil, litter, dead wood, dung).
  2. Record species, substrate, host tree and GPS; photograph cap, gills, stem and base.
  3. Collect one specimen of unknowns in a labelled paper bag, never plastic.
  4. Take spore prints the same night.
  5. Keep going for 3 autumns; single season lists miss most species.
Kit list

The day one mistake: Recording without the base of the stem. The volva and base are often the identifying feature.

Fungi, lichens and bryophytes

Grassland fungi (waxcap) surveys

Count waxcaps, clubs, corals and earthtongues to grade grassland conservation value.

Answers
How many?, How is it spread across the area?
Effort
Whole field walked in 5 m strips, 3 visits between September and November, after rain
Season
Sep, Oct, Nov (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None usually.
How to run it
  1. Survey unimproved, short grazed or mown swards after autumn rain.
  2. Walk strips 5 m apart; record species and count fruiting bodies per species.
  3. Photograph and collect one of each unknown.
  4. Use the highest species count of the 3 visits for grading.
  5. Record management and sward height.
Kit list

The day one mistake: One visit in October. Fruiting shifts weeks between years; three visits or nothing.

Fungi, lichens and bryophytes

Lichen and bryophyte quadrats on trees and rock

Record lichen and moss cover on trunks or rock faces in small quadrats. Used for air quality indices and epiphyte monitoring.

Answers
Is it here?, How many?
Effort
Ladder quadrats of 10 cm x 50 cm on 4 aspects of 10 or more trees per site, one visit
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None; do not collect on protected sites without consent.
How to run it
  1. Select trees of similar species and girth; record aspect and bark pH class.
  2. Place the ladder quadrat at 1 to 1.5 m on each aspect.
  3. Record presence of each species in each 10 cm square; estimate cover.
  4. Photograph each quadrat.
  5. Collect tiny fragments only when needed for microscopy.
Kit list
  • Ladder quadrat (make from acetate, or British Lichen Society)
  • Hand lens 10x and 20x
  • Lichen guide (FSC or NHBS (UK); a bookshop (US))

The day one mistake: Sampling only the sunny side. Aspect drives the community; record all four.

Fungi, lichens and bryophytes

Bryophyte relevés

Full moss and liverwort lists with cover in small plots by substrate. Slow, specialist work that mostly happens at home under the microscope.

Answers
Is it here?, How is it spread across the area?
Effort
Plots of 1 m2 to 4 m2 in each microhabitat, one visit, best in wet seasons
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None; consent on protected sites.
How to run it
  1. Sample each substrate separately: soil, rock, bark, dead wood, flush.
  2. Record cover per species; collect small packets of unknowns.
  3. Label packets with plot, substrate and date immediately.
  4. Identify with a microscope and keys.
  5. Record moisture and shade.
Kit list

The day one mistake: Collecting whole cushions. A thumbnail sized piece identifies as well and leaves the colony alive.

Marine mammals and seabirds

Land based cetacean watches

Scan a fixed sea area from a headland with binoculars and record every cetacean sighting with bearing, distance and behaviour. Cheap, repeatable, and the basis of most citizen science cetacean schemes.

Answers
Is it here?, How many?, What is it doing?
Effort
Fixed headland vantage point, 1 to 2 hour watches with a scan every 15 minutes, weekly or fortnightly, sea state 3 or less
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None for observation.
How to run it
  1. Choose a headland with height and a wide view; mark the sector limits on a photo.
  2. Record effort every 15 minutes: sea state, swell, visibility, glare, observers.
  3. Scan slowly with the naked eye, then binoculars; on a sighting record time, species, number, calves, bearing (compass) and distance (reticle or estimated).
  4. Note behaviour (travelling, feeding, milling, bow riding) and direction.
  5. Stop effort above sea state 3 or when visibility drops below 1 km.
Kit list
  • Binoculars 8×42
  • Sighting compass (Suunto or Silva, via an outdoor shop)
  • Reticle binoculars 7×50 (Steiner or Fujinon, via an optics dealer)
  • Tally counter

The day one mistake: Recording sightings without recording effort. Ten dolphins in a two hour watch and ten dolphins in a ten hour watch are different answers.

Marine mammals and seabirds

Seal haul out counts

Count seals hauled out on a known site at low water, by species and age class, without disturbing them. Used for population trends and pup production.

Answers
How many?, What is it doing?, How is it spread across the area?
Effort
Counts from a vantage point or boat at 200 m or more, within 2 hours either side of low water; harbour seals during the August moult, grey seal pups October to December, plus monthly trend counts
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None from land at a distance. Approaching haul outs by boat or drone may need a licence and always needs a disturbance protocol.
How to run it
  1. Time the count to low water; seals haul out on the falling tide.
  2. Count from a fixed point at 200 m or more with a telescope; count twice and record both.
  3. Record species, age class (pup, juvenile, adult), and any tagged or marked animals.
  4. Note disturbance events (boats, dogs, walkers) and the seals' response.
  5. Repeat in the pupping and moult seasons for the species; grey and harbour seals differ by months.
Kit list

The day one mistake: Getting close enough to flush them. A stampede into the water can crush pups and empties the site for hours; if heads come up, you are too close.

Marine mammals and seabirds

Photo identification (mark recapture)

Photograph dorsal fins, flukes or scars and match individuals across encounters. Gives abundance by mark recapture, site fidelity and social structure.

Answers
How many?, What is it doing?, How is it spread across the area?
Effort
Boat surveys on fixed routes, 20 or more encounters per season, every individual in each group photographed, catalogue matched
Season
Apr, May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Funded project
Licence
Research permit and vessel permit in most waters; approach distances are law in many countries.
How to run it
  1. Approach groups slowly from the side and behind, never through them; obey the local approach distance.
  2. Photograph every animal in the group, both sides of the fin, at right angles; take more frames than you think you need.
  3. Record encounter start and end, GPS, group size, calves and behaviour.
  4. Grade every photo for quality before matching; only good grades enter the analysis.
  5. Match to the catalogue by fin notches and scars; two people confirm every match.
Kit list

The day one mistake: Photographing only the animals that come to the boat. Missing the shy ones biases abundance every time.

Marine mammals and seabirds

Passive acoustic monitoring (hydrophones and click detectors)

Moored detectors log echolocation clicks or full audio around the clock, giving presence and activity through weather and darkness that visual surveys miss.

Answers
Is it here?, Where does it occur across sites?, How is it spread across the area?
Effort
Moored click detectors or recorders at fixed stations for 1 to 3 months per deployment, serviced on each retrieval
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Funded project
Licence
Marine licence or seabed consent for moorings in many countries; vessel permits.
How to run it
  1. Choose stations that answer the question (harbours, tidal races, wind farm sites); one detector per station.
  2. Moor with a weight, riser and surface or acoustic release; log GPS, depth and deployment time.
  3. Service and download on retrieval; check battery and memory before redeployment.
  4. Run the manufacturer's click train classifier, then verify a sample by hand.
  5. Report detection positive minutes or hours per day as the standard unit.
Kit list

The day one mistake: Reporting raw click counts. Use detection positive minutes so stations and months can be compared.

Birds

Waterbird core counts (wetland counts)

Count every waterbird on a defined wetland or estuary sector once a month through the non breeding season. The method behind national wetland bird schemes.

Answers
How many?, How is it spread across the area?
Effort
Monthly all year, priority September to March, all counters on the same date, 2 h either side of high tide on estuaries, daytime; US and international January counts are a single midwinter count
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None.
How to run it
  1. Fix the sector boundaries on a map and never change them.
  2. Count on the scheme's priority date; on estuaries count at high tide when birds are concentrated.
  3. Scan systematically with a telescope; count large flocks in blocks of 10, 50 or 100.
  4. Record every species, including gulls if the scheme asks, and note disturbance and ice cover.
  5. Submit to the national scheme the same week.
Kit list

The day one mistake: Counting a flock twice as it moves between sectors. Agree the sector edges with neighbouring counters and note the time each flock was counted.

Birds

Vantage point watches (raptors and flight lines)

Watch a fixed arc from a high point and map every flight of target species with height and duration. The standard for wind farm and infrastructure impact assessment.

Answers
What is it doing?, How is it spread across the area?, How many?
Effort
36 h per season per vantage point (wind farm standard), watches of up to 3 h, viewing arc of 180 degrees, all seasons
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None for observation. Schedule 1 species near the nest need a licence.
How to run it
  1. Choose vantage points that together cover the whole site within 2 km; mark the arc on a map.
  2. Watch for up to 3 h per session, scanning continuously; record effort in 15 minute blocks.
  3. On a flight: species, number, start time, duration in 15 second intervals, height band, and the route drawn on the map.
  4. Record weather every 15 minutes; stop in poor visibility.
  5. Spread the 36 h across the season and across the day.
Kit list

The day one mistake: Forgetting the flight height. Collision risk models need the seconds spent in the rotor height band, not just the sighting.

Bats

Hibernation site counts

Count and identify torpid bats in caves, mines, tunnels and cellars in winter. Licensed work; you will assist a licence holder.

Answers
Is it here?, How many?
Effort
One or two visits per winter, mid December to February, small trained team, under 1 h inside
Season
Dec, Jan, Feb (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Licence required: UK Natural England bat survey class licence Level 2 or a bespoke licence; US state permits plus WNS decontamination. Cave and mine access permission.
How to run it
  1. Enter only with the licence holder; keep the team small and quiet.
  2. Search every crevice systematically with a torch and mirror; do not touch or breathe on bats.
  3. Identify to species where possible without handling; record 'bat species' if unsure.
  4. Log temperature and humidity at each section.
  5. Leave within the hour; one visit per month at most.
  6. North America: follow the USFWS white nose syndrome decontamination protocol for every item that enters a roost or touches a bat.
Kit list

The day one mistake: Waking bats with heat and light. Each arousal burns a week of fat reserves; keep lights low and visits short.

Small mammals

Dormouse nest tubes and nest boxes

Nest tubes and boxes on branches attract hazel dormice to build nests. Standard presence survey and long term monitoring.

Answers
Is it here?, Where does it occur across sites?
Effort
50 nest tubes per site on a 20 m grid or along hedges, checked monthly April to November, 1 season for presence
Season
Apr, May, Jun, Jul, Aug, Sep, Oct, Nov (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
England and Wales: opening tubes and boxes that may hold dormice needs the dormouse survey class licence or a licence holder present; installing tubes does not. Elsewhere in Europe: national protected species rules.
How to run it
  1. Fix tubes under horizontal branches at 1 to 2 m, entrance toward the trunk, 20 m apart.
  2. Check monthly; open tubes gently and look for the woven nest of shredded honeysuckle or grass.
  3. Nest boxes are checked by licence holders only; record animals, sex, weight and torpor.
  4. Score effort with the scheme's index; a full season of 50 tubes is needed before absence is claimed.
  5. Remove tubes in winter.
Kit list
  • Dormouse nest tubes (NHBS (UK) or Forestry Suppliers (US))
  • Nest boxes (NHBS (UK) or Forestry Suppliers (US))
  • Handheld GPS

The day one mistake: Confusing a wood mouse or bird nest with a dormouse nest. Dormouse nests are woven, with no cup and often green leaves; photograph every nest.

Small mammals

Water vole and riparian mammal sign surveys

Search banks for burrows, latrines, feeding stations and prints of water voles, and for otter spraints and beaver sign. The standard riparian mammal method.

Answers
Is it here?, How is it spread across the area?, How many?
Effort
Both banks walked for 500 m sections, 2 visits (spring and late summer), searched within 2 m of the water
Season
Apr, May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Water voles and otters are protected in the UK; sign surveys need no licence, disturbing burrows does.
How to run it
  1. Walk the bank slowly in waders where safe, checking the waterline and 2 m up the bank.
  2. Record latrines, burrows with grazed lawns, feeding stations of cut stems at 45 degrees, and footprints.
  3. GPS every sign; count latrines per 100 m as the abundance index.
  4. Otter: record spraints on prominent rocks and under bridges; beaver: cut stems, dams and lodges.
  5. Two visits at least 2 weeks apart before recording absence.
Kit list

The day one mistake: Surveying after a flood. Sign is washed away and you will record a false absence; wait 2 weeks.

Medium and large mammals

Hair snares and scat DNA sampling

Collect hair on barbed wire corrals or scat along transects and identify individuals by DNA for capture recapture. Standard for bears, wolves, lynx and cats.

Answers
How many?, Is it here?, How is it spread across the area?
Effort
Hair corrals on a 5 to 7 km grid checked every 10 to 14 days for 4 sessions; scat transects walked each session
Season
May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Funded project
Licence
Permits for lure use and sampling; export permits for DNA samples in many countries.
How to run it
  1. Build barbed wire corrals at 50 cm height around a scent lure; one per grid cell.
  2. Check every 10 to 14 days; collect each hair clump into a separate envelope with new gloves and tweezers; flame the wire.
  3. Walk scat transects the same session; sample the outside of fresh scat into ethanol or silica.
  4. Record GPS, date, session and sample ID; keep samples dry and cool.
  5. Genotype at the lab; run spatial capture recapture.
Kit list

The day one mistake: Mixing hair from two clumps in one envelope. One clump, one envelope, or the lab gets a mixed genotype and bins it.

Medium and large mammals

Drone and aerial surveys

Fly pre planned strips with an RGB or thermal camera and count animals on the imagery. Fast for open country herds, colonies and haul outs.

Answers
How many?, How is it spread across the area?
Effort
Systematic strips at 60 to 120 m altitude, 10 to 30 percent overlap, early morning or with thermal at night, 2 to 3 repeats
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
Pilot registration or licence in almost every country; protected area permits; disturbance rules for seals and colonies.
How to run it
  1. Plan the flight in the mission app with strip spacing that matches the camera footprint; check airspace and wildlife disturbance rules.
  2. Fly at the altitude the target species tolerates (test and watch for reactions); keep the sun behind the camera.
  3. Count on the imagery with a grid overlay, two counters independently.
  4. Record altitude, time, camera and weather for every flight.
  5. Repeat flights to estimate detection.
Kit list

The day one mistake: Flying over a colony at 30 m. Birds and seals flush, the count is ruined and the permit is at risk; find the tolerated altitude first.

Reptiles

Sea turtle nesting beach surveys

Walk the beach at dawn counting fresh tracks and nests by species, and patrol at night to tag females and protect nests.

Answers
How many?, What is it doing?, How is it spread across the area?
Effort
Beach walked every morning at dawn through the nesting season, plus night patrols for tagging; May to October in the north Atlantic and Mediterranean, year round or shifted in the tropics and Indo Pacific, so the six month rule does not apply here
Season
May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Permit required everywhere for tagging and nest handling; beach access agreements.
How to run it
  1. Walk the full beach at first light; record every crawl as a nest or false crawl by the track pattern.
  2. Identify species by track width and gait; mark and GPS each nest; camouflage the site.
  3. Night patrols: approach a nesting female from behind only once she is laying; tag and measure.
  4. Record hatching and excavate nests 3 days after emergence to count shells and unhatched eggs.
  5. Report to the regional network.
Kit list

The day one mistake: Using white light on the beach. Females abort nesting and hatchlings head inland; red light only, and only when needed.

Fish

Seine netting

Drag a weighted net through shallow water to enclose fish over a known area. Good for juveniles in margins, lakes and estuaries.

Answers
How many?, Is it here?
Effort
3 to 5 hauls per site over a fixed area, daylight, 2 to 3 people
Season
May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
Fisheries authority consent in most countries.
How to run it
  1. Measure the haul area; keep the lead line on the bottom throughout.
  2. Walk the net in a semicircle back to the bank and close it quickly.
  3. Process in aerated buckets: species, length, count; release.
  4. Record area swept and haul number.
  5. Rinse and dry the net between waters.
Kit list

The day one mistake: Lifting the lead line early. The fish leave under the net and the haul is meaningless.

Fish

Salmonid redd counts

Count and map the gravel nests (redds) of spawning salmon and trout to index spawning stock.

Answers
How many?, How is it spread across the area?
Effort
Whole reach walked upstream every 7 to 10 days through the spawning season, clear water, polarised glasses
Season
Oct, Nov, Dec, Jan (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None for counting; river access permission.
How to run it
  1. Walk upstream in clear water; polarised glasses and low sun angle.
  2. Record each redd: GPS, size, species if known, and flag it to avoid recounting.
  3. Note superimposed redds and test digs separately.
  4. Repeat every 7 to 10 days; the sum of new redds is the season total.
  5. Avoid wading through redds.
Kit list

The day one mistake: Counting the same redd twice on the next visit. Flag or GPS every redd on the day you find it.

Plants and vegetation

Aquatic macrophyte surveys

Record submerged, floating and emergent plants in a water body by wading, grapnel and bathyscope. Used for water quality and habitat classification.

Answers
Is it here?, How many?
Effort
100 m river sections or whole pond, grapnel throws every 10 m, wading and bankside searches, one visit in mid summer
Season
Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None; disinfect kit between waters against invasive species.
How to run it
  1. Survey in June to September when plants are fully grown.
  2. Walk the bank and wade where safe; throw a grapnel every 10 m and record what comes up.
  3. Use a bathyscope to see the bed in deeper water.
  4. Record species with cover scores and the percent of channel shaded.
  5. Bag unknowns in water for identification.
Kit list
  • Grapnel (NHBS (UK) or a boat chandler)
  • Bathyscope (NHBS (UK) or Forestry Suppliers (US))
  • Waders
  • Aquatic plant guide (FSC or NHBS (UK); a bookshop (US))

The day one mistake: Recording only what you can see from the bank. Half the species are submerged; throw the grapnel.

Plants and vegetation

Rare plant population counts

Census a rare plant population by counting individuals, flowering stems or occupied grid cells on a fixed date each year.

Answers
How many?, How is it spread across the area?, What is it doing?
Effort
Whole colony counted or sampled in permanent plots once a year at peak flowering
Season
May, Jun, Jul, Aug (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Protected plants must not be picked or uprooted; consent on protected sites.
How to run it
  1. Map the colony boundary with GPS and mark permanent corners.
  2. Count every individual or flowering stem; for large colonies count in fixed quadrats and scale up.
  3. Record life stage (seedling, vegetative, flowering) and threats (grazing, trampling, shading).
  4. Photograph from fixed points.
  5. Same week each year.
Kit list

The day one mistake: Counting flowers instead of plants without saying which. Fix the count unit in year one and never change it.

Terrestrial invertebrates and pollinators

Soil and litter extraction (Tullgren funnels and earthworm surveys)

Collect soil or litter samples and drive the animals out with heat and light into alcohol, or dig and hand sort pits for earthworms. The way into the least surveyed group of all.

Answers
How many?, Is it here?
Effort
5 to 10 soil cores or litter samples per site; 20 cm x 20 cm x 10 cm earthworm pits; extraction over 3 to 7 days
Season
Mar, Apr, May, Sep, Oct, Nov (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None.
How to run it
  1. Take cores or litter quadrats of fixed size; record depth and moisture.
  2. Place samples in Tullgren funnels under a lamp for 3 to 7 days over 70 percent ethanol.
  3. For earthworms, dig a 20 cm pit and hand sort, then pour mustard solution to bring up deep burrowers.
  4. Identify to order or family; count per sample.
  5. Standardise sample size so counts are per square metre.
Kit list

The day one mistake: Drying samples too fast. Turn the lamp up slowly over days or the animals die in the soil instead of dropping.

Marine benthos, reefs and shore

Benthic grab and core sampling

Lower a grab or corer to the seabed to sample sediment infauna, sieve it and identify the animals. The core method for soft sediment monitoring.

Answers
How many?, Is it here?, How is it spread across the area?
Effort
3 to 5 replicate grabs per station, sieved on deck at 1 mm or 0.5 mm, stations on a grid
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Funded project
Licence
Vessel permits; protected area consent; formalin handling rules.
How to run it
  1. Position the vessel on station; record GPS and depth for every grab.
  2. Accept a grab only if it is full and undisturbed; record the volume.
  3. Sieve gently on deck; fix the residue in buffered formalin or ethanol.
  4. Take a sediment subsample for particle size and organic content.
  5. Sort and identify in the lab; count per grab area.
Kit list

The day one mistake: Keeping a half full grab. It means the jaws hit a stone and the sample is biased; drop it and repeat.

Marine benthos, reefs and shore

Coral reef line intercept and photo quadrats

Record what lies under a tape or in photo quadrats along a reef transect to measure coral cover, bleaching and algae. Paired with fish counts on the same tape.

Answers
How many?, How is it spread across the area?, What is it doing?
Effort
20 m or 50 m transects at fixed depths, 3 to 5 per site, benthos recorded under the tape every 0.5 m or in photo quadrats every 1 m
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Funded project
Licence
Dive certification; marine park and research permits.
How to run it
  1. Lay the tape along the depth contour; record depth and GPS at both ends.
  2. Line intercept: record the benthic category under every point or the length of each patch under the tape.
  3. Photo quadrats: photograph a 1 m frame every metre with the camera held square.
  4. Note bleaching, disease and recent mortality by colony.
  5. Analyse photos with point count software; same transects every year.
Kit list

The day one mistake: Recording 'coral' without the growth form or genus. Cover alone hides the shift from branching to massive corals that the reef is telling you about.

Bats

Preliminary roost assessment and tree inspection

Look for droppings, staining, feeding remains and roost features by day, and grade the structure for bat suitability. The first step of every bat job and the basis for deciding how many emergence surveys follow.

Answers
Is it here?
Effort
Daytime external and internal inspection of buildings and trees, 1 to 3 h per structure, any month; ground level tree assessment then aerial inspection with an endoscope
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Entering a known roost or handling bats needs a licence (UK class licence; US state permits). Assessment from outside and of empty structures does not.
How to run it
  1. Inspect the outside: gaps under tiles, soffits, lead flashing, lifted bark, woodpecker holes, rot holes; photograph every feature.
  2. Inside: torch lofts and cellars for droppings, urine staining, moth wings and live bats; crumble droppings to tell bat from mouse.
  3. Trees: ground level assessment with binoculars, then climb or use a pole camera and endoscope for potential roost features.
  4. Grade suitability (negligible, low, moderate, high) using the BCT guidelines and set the number of emergence surveys from the grade.
  5. Never block or disturb a feature; record and leave.
Kit list

The day one mistake: Grading a tree from the ground as negligible. Half the roost features are invisible from below; climb or pole camera before you write it off.

Medium and large mammals

Badger bait marking

Feed peanuts mixed with coloured plastic pellets at each sett, then map which colours appear in which latrines to draw territory boundaries.

Answers
How is it spread across the area?, What is it doing?
Effort
Bait with coloured pellets at each sett for 10 to 14 days, then search latrines for 2 to 3 days, late winter to spring
Season
Feb, Mar, Apr (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Badgers and setts are protected in the UK; baiting outside setts needs no licence, disturbing setts does.
How to run it
  1. Assign a unique pellet colour to each main sett; mix pellets with peanuts and syrup.
  2. Bait daily for 10 to 14 days near the sett entrances.
  3. Search all latrines within 500 m of every sett for 2 to 3 days; record the colours found.
  4. Draw territory boundaries between latrines holding different colours.
  5. Do this before vegetation grows, February to April.
Kit list

The day one mistake: Using the same colour for two setts within 1 km. The map becomes unreadable and the fortnight is wasted.

Amphibians

Artificial cover objects for terrestrial salamanders

Untreated wooden boards laid on the forest floor draw terrestrial salamanders that no other method finds. The core North American method for woodland amphibians.

Answers
Is it here?, How many?, Where does it occur across sites?
Effort
Grids or transects of 20 to 50 boards per site, left 4 weeks before checking, checked every 2 to 4 weeks in cool wet weather
Season
Mar, Apr, May, Sep, Oct, Nov (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Permits for handling in some US states; none for checking boards.
How to run it
  1. Lay untreated boards (30 x 30 cm to 60 x 60 cm) flat on cleared soil in a grid; GPS each board.
  2. Leave 4 weeks before the first check.
  3. Lift boards in cool damp conditions; record species, count, and life stage; replace the board exactly.
  4. Handle animals with wet hands or not at all; never on dry or hot days.
  5. Same boards, same interval, every year.
Kit list

The day one mistake: Checking on a dry afternoon. Salamanders are underground and the board is empty; wait for rain.

Reptiles

Freshwater turtle hoop net trapping

Baited hoop nets set in shallow water catch freshwater turtles for marking and population estimates. The standard North American turtle method.

Answers
Is it here?, How many?
Effort
5 to 10 baited hoop nets per water body, set for 3 to 5 nights, checked daily
Season
Apr, May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
State scientific collecting permit in the US; protected species need extra permits.
How to run it
  1. Set nets in 0.5 to 1 m of water with the top of the hoop above the surface so turtles can breathe.
  2. Bait with sardines in a perforated can; stake the net so it cannot roll.
  3. Check every 24 hours; process species, sex, carapace length, notch marks; release at the net.
  4. Record trap nights.
  5. Disinfect nets between water bodies.
Kit list

The day one mistake: Setting the hoop fully submerged. Turtles drown by morning.

Fish

Multi mesh gill netting (lakes)

Standardised multi mesh gill nets set overnight across depth strata give a repeatable picture of lake fish communities. Lethal for most fish, so used only where the standard demands it.

Answers
How many?, Is it here?
Effort
Nordic multi mesh nets set overnight, number of nets scaled to lake area and depth strata (CEN EN 14757), 1 to 3 nights
Season
Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Funded project
Licence
Fisheries authority permit everywhere; ethics approval because the method kills fish.
How to run it
  1. Plan net numbers per depth stratum from the lake area and maximum depth using the CEN table.
  2. Set nets at dusk at random positions in each stratum; record GPS and depth.
  3. Lift at dawn; identify, count and measure every fish by mesh panel.
  4. Record net nights per stratum.
  5. Dry and disinfect nets between lakes.
Kit list

The day one mistake: Setting all nets in the shallows. Without the deep strata the lake community is misread.

Freshwater invertebrates and plankton

Pond invertebrate netting (timed hand net)

A timed hand net sample across all pond habitats, sorted live, gives the family list behind pond quality scores such as PSYM.

Answers
Is it here?, How many?
Effort
3 minute net sample split across every mesohabitat in the pond, 1 visit in late spring or summer
Season
May, Jun, Jul (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None; consent on protected sites.
How to run it
  1. Map the mesohabitats (open water, emergent, submerged, margins) and divide the 3 minutes among them by area.
  2. Net in short sweeps, emptying into a tray between habitats.
  3. Sort live in white trays for 1 hour, or preserve in 70 percent alcohol.
  4. Identify to family; record presence or abundance class.
  5. Disinfect and dry the net before the next pond.
Kit list

The day one mistake: Netting only the open water. Most families live in the marginal vegetation.

Terrestrial invertebrates and pollinators

Bumblebee transects and flower insect timed counts

Walk a fixed route counting bumblebees by species and caste, or watch a flower patch for ten minutes counting every insect visitor. The two methods behind the UK Pollinator Monitoring Scheme.

Answers
How many?, How is it spread across the area?
Effort
BeeWalk: fixed 1 to 2 km transect walked monthly March to October; FIT count: 10 minutes watching a 50 x 50 cm flower patch, any sunny day
Season
Mar, Apr, May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None.
How to run it
  1. BeeWalk: walk at a steady pace recording bumblebees within 2 m either side and 4 m ahead, by species and caste (queen, worker, male).
  2. Weather: above 13 C, dry, light wind, between 11:00 and 17:00.
  3. FIT count: choose a target flower, mark a 50 x 50 cm patch, count every insect that lands in 10 minutes by group.
  4. Record the flower species and its cover.
  5. Monthly for BeeWalk; FIT counts as often as you like.
Kit list

The day one mistake: Counting honeybees as bumblebees. Learn the three common bumblebees first and record unknowns as unknown.

Freshwater invertebrates and plankton

Benthic diatom sampling

Brush the biofilm off submerged stones, preserve it and identify the diatoms under a microscope. The standard for river nutrient and acid status (TDI, EU Water Framework Directive).

Answers
Is it here?, How many?
Effort
5 cobbles per site scrubbed into 50 ml, or 10 plant stems where no stones, 1 to 2 visits per year, spring and autumn
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None for sampling; some protected rivers need consent.
How to run it
  1. Pick 5 cobbles from the well lit, flowing part of the reach, not from shaded margins or silt.
  2. Scrub the upper surface with a toothbrush into a tray of river water; pour into a 50 ml bottle.
  3. Preserve with Lugol or ethanol; label site, date and substrate.
  4. Prepare slides by acid digestion in the lab; count 300 valves per sample.
  5. Calculate the index; record which substrate was used because the index differs.
Kit list

The day one mistake: Scrubbing silt covered stones. The community on silt is a different one and the index is wrong; choose clean, flowing sites.

Freshwater invertebrates and plankton

Phytoplankton and chlorophyll sampling

Sample the water column for phytoplankton counts and chlorophyll a to track algal blooms and lake trophic state.

Answers
How many?, Is it here?
Effort
Integrated tube sample of the surface mixed layer at 1 to 3 stations, monthly through the growing season, filtered the same day
Season
Apr, May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
None for sampling; reservoirs and drinking water lakes need the operator's permission. Cyanobacteria blooms: gloves, no skin contact.
How to run it
  1. Lower a weighted tube through the mixed layer (or to twice the Secchi depth) and collect the integrated sample.
  2. Subsample 250 ml into a dark bottle for counts and preserve with Lugol; keep 1 L cold for chlorophyll.
  3. Filter the chlorophyll sample through a glass fibre filter within 8 hours; freeze the filter.
  4. Record Secchi depth, temperature profile and any surface scum or colour.
  5. Count cells in a sedimentation chamber; report cells per ml and chlorophyll in micrograms per litre.
Kit list

The day one mistake: Sampling only the surface film. A scum on top and a clear column below give a bloom count ten times too high.

Marine benthos, reefs and shore

Seaweed and kelp surveys

Record seaweed species and cover on the shore, and kelp density and canopy along belts below the tide, to track habitat condition and non native species.

Answers
How many?, How is it spread across the area?, Is it here?
Effort
Shore: quadrats along a vertical transect at spring low tide; kelp: 25 m x 2 m belt transects by snorkel or dive at 2 to 3 depths, once per season
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None on open shores; marine protected areas and kelp harvesting zones need permits; diving needs certification.
How to run it
  1. Shore: run the transect from the lichen zone to low water; at each height record every seaweed species and percent cover in a 0.5 m quadrat.
  2. Note zonation (channel wrack, spiral wrack, bladder wrack, serrated wrack, kelp) and photograph each quadrat.
  3. Kelp: swim a 25 m tape at fixed depth; count stipes per 2 m belt and estimate canopy cover; measure 10 stipe lengths.
  4. Record non natives (wireweed, Undaria) separately with GPS.
  5. Collect a fragment of any unknown into a bag of seawater; press or photograph it the same day.
Kit list

The day one mistake: Surveying on a neap tide. The kelp zone stays under water and half the shore is never seen; use the lowest spring tides.

Birds

Breeding wader surveys (Brown and Shepherd)

Walk the whole site in daylight so that no ground is more than 100 m away, mapping every wader and its behaviour, then estimate breeding pairs from the three visits. The Brown and Shepherd method is the standard for lapwing, curlew, snipe, redshank and golden plover on open ground.

Answers
How many?, How is it spread across the area?, What is it doing?
Effort
2 visits (a third is optional for curlew), visit 1 mid April to mid May and visit 2 mid May to end of June, at least 7 days apart, whole site walked within 100 m of every point, 08:30 to 18:00
Season
Apr, May, Jun, Jul (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None for observation in the UK or the US. Breeding waders are Schedule 1 in parts of the UK (for example dotterel), so do not search for nests without a licence. Landowner permission is needed to walk fields with stock.
How to run it
  1. Prepare a map at 1:10,000, plan a route with legs no more than 200 m apart, and walk it between 08:30 and 18:00 avoiding rain and wind above Beaufort 4.
  2. Make visit 1 between mid April and mid May and visit 2 between mid May and the end of June, at least 7 days apart, adding a third visit in June only where curlew or chicks need confirming.
  3. Mark every wader on the map with a species code and a behaviour code: alarm calling, display, distraction display, incubating, chicks seen, or just present.
  4. Walk steadily and do not stop to search for nests; if you flush an incubating bird move on at once so it can return.
  5. Count pairs per species using the standard rule set: for lapwing use the highest visit count of displaying or alarming adults, for curlew and snipe use the highest count from visits 1 and 2, and treat birds with chicks as confirmed.
Kit list

The day one mistake: Surveying at dawn like a songbird count. Waders are least visible at dawn and the method relies on birds standing and alarming in the middle of the day; keep to the 08:30 to 18:00 window or your counts will be low and inconsistent.

Birds

Nest box monitoring (tits, flycatchers, owls, kestrels)

Check numbered nest boxes on a weekly schedule to record first egg date, clutch size, hatching and fledging under the BTO Nest Record Scheme or NestWatch (Cornell) rules. The easiest way to get breeding success data for hole nesters and the source of most long term studies on climate and timing.

Answers
What is it doing?, How many?
Effort
20 to 50 small boxes per site checked every 7 days from late March to July, raptor and owl boxes checked 2 to 4 times per season, under 2 minutes per box
Season
Mar, Apr, May, Jun, Jul, Aug (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
In the UK checking boxes of most species (including kestrel and tawny owl) needs no licence, but barn owl and any other Schedule 1 species need a Natural England, NatureScot or NRW licence to disturb at the nest. In the US boxes of native species may be checked without a permit; handling or banding needs a federal banding permit.
How to run it
  1. Erect boxes by February, numbered and mapped, 2 to 4 m up, entrance away from prevailing weather and direct sun, small boxes 20 to 30 m apart.
  2. Start weekly checks in late March; approach quietly, tap the box, open the lid for a few seconds, count contents and close.
  3. Record stage as nest building, lining, eggs (count), young (count and age) or empty, and back calculate first egg date assuming one egg per day.
  4. When chicks are close to fledging (day 12 onwards in tits) do not open the box; watch from a distance or check after they have gone.
  5. Clean out old nests after the season ends and enter every record online so it reaches the national scheme.
Kit list

The day one mistake: Opening a box with large chicks inside. Tit chicks from about day 12 will explode out of the box and cannot be put back; note the hatch date and stop opening the box a week before the expected fledge date.

Birds

Heronry and colonial waterbird counts

Count apparently occupied nests in heronries, egret colonies, cormorant colonies and other tree or reed nesting waterbirds from outside the colony. The BTO Heronries Census has run since 1928 and the Colonial Waterbird Survey in the US follows the same logic.

Answers
How many?, How is it spread across the area?, What is it doing?
Effort
1 to 3 visits per colony between late March and mid May, count apparently occupied nests from a fixed vantage point, 30 to 90 minutes per visit
Season
Mar, Apr, May, Jun (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Counting from outside a colony needs no licence in either country. Entering a colony to count nests or check contents disturbs Schedule 1 species in the UK (for example little egret in some listings) and colonial waterbirds under the MBTA in the US; stay outside unless licensed.
How to run it
  1. Locate the colony from records or by following birds carrying sticks in March, and find a vantage point outside the trees where most nests are visible.
  2. Count before leaves close the canopy: late March to mid April for grey heron in the UK, later for species that nest in reeds or leaf cover.
  3. Count apparently occupied nests, meaning nests that are well built with an adult or young on or beside them, and score the rest as unoccupied.
  4. Repeat the count on a second visit at the peak of incubation and use the highest count of occupied nests as the colony figure.
  5. Keep to the edge, keep visits under 90 minutes and leave at once if adults start leaving nests, then submit the count to the national scheme.
Kit list

The day one mistake: Counting every nest structure. Old nests persist for years and a heronry with 30 nests may hold 12 pairs; only count nests that are occupied or freshly built with whitewash under them.

Birds

Visible migration counts (fixed point)

Stand at a fixed point on a headland, ridge or valley edge and count every bird flying through with its direction, in hourly blocks. The Trektellen network in Europe and hawkwatch sites in the US (HMANA protocol) use exactly this method.

Answers
How many?, What is it doing?, How is it spread across the area?
Effort
1 fixed point, watched from first light for 3 to 4 hours, totals logged per hour, as many mornings as possible in peak passage (September to November, March to May)
Season
Mar, Apr, May, Aug, Sep, Oct, Nov (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None for observation in the UK or the US. Access to headlands and nature reserves may need permission before dawn.
How to run it
  1. Pick a point with a wide view along the expected flight line, note its coordinates, and use the same spot every time.
  2. Arrive 15 minutes before sunrise, log start time, cloud, wind direction and strength, and visibility.
  3. Count every bird that passes in one direction, in the hourly block it passes, and record direction of travel; birds that land and stay are logged separately as grounded.
  4. Log counting effort to the minute, including breaks, because the count is expressed as birds per hour.
  5. Submit hourly totals to Trektellen or HawkCount the same day and check that the species totals match your notebook.
Kit list

The day one mistake: Counting the same flock that circles back. Only log birds moving through in the main direction of passage and note any large flock that reappears, or your finch totals will double.

Birds

Low tide feeding distribution and high tide roost counts (WeBS Low Tide Counts)

Count waders and wildfowl on the intertidal flats around low water so their feeding distribution can be mapped, and count the roosts at high water so the total population is known. The WeBS Low Tide Count scheme in the UK follows this method; in the US the same design is used in shorebird site assessments.

Answers
How is it spread across the area?, How many?, What is it doing?
Effort
Estuary divided into count sectors, each counted once per month November to February within 2 hours either side of low water; roosts counted within 2 hours of high water, 1 to 3 hours per sector
Season
Nov, Dec, Jan, Feb (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None for observation. Walking onto intertidal flats may be restricted on nature reserves and military ranges in both countries; check access and safety rules before going out.
How to run it
  1. Divide the estuary into sectors on a map using creeks, groynes and marks that you can see in the field, and assign each sector a code.
  2. Count each sector once a month from November to February, starting 2 hours before low water and finishing 2 hours after, on a rising or falling tide as the scheme specifies.
  3. Record every wader and wildfowl in the sector by species with a count and a behaviour code (feeding, roosting, flying through).
  4. On a separate date count the high tide roosts within 2 hours of high water from a distance that does not flush the birds.
  5. Note the tide height, weather and any disturbance, and submit counts to the scheme organiser after each visit.
Kit list

The day one mistake: Counting at low water on a spring tide when the flats extend for miles and then again on a neap tide when they do not. Count on similar tide heights each month or your distribution maps will move with the tide instead of the birds.

Birds

Colour ring and tag resighting

Read the colour rings, flags and wing tags of marked birds and report them to the ringing scheme so survival, movements and site fidelity can be estimated. The cheapest way to contribute to a national scheme; every accurate resighting is a data point.

Answers
What is it doing?, How is it spread across the area?, How many?
Effort
Repeated visits to roosts and feeding sites, 1 to 3 hours per visit, every ring read from a scope at 30 to 100 m, all year with weekly visits in season
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None for observation in either country. Catching a bird to read a metal ring needs a BTO permit in the UK or a federal banding permit in the US.
How to run it
  1. Learn the ring positions and colour codes for the species you will see; the European colour ring birding site and the USGS Bird Banding Lab list every scheme.
  2. Set up a scope at a roost or feeding flock, scan legs systematically and read each combination twice before writing it down.
  3. Record the full combination in the standard format (left leg above and below the joint, then right leg), the date, place, time and what the bird was doing.
  4. Photograph the bird whenever you can; a photo settles a doubtful read.
  5. Submit sightings to the scheme coordinator listed on the cr birding site, or to the BTO Ringing Scheme (ring.ac) for the UK and the Bird Banding Laboratory (reportband.gov) for the US, and keep the reply, which tells you where the bird was ringed.
Kit list

The day one mistake: Guessing a ring you could not fully read. A wrong resighting creates a movement that never happened and can survive in the dataset for years; record a partial read as partial and move on.

Birds

Avian influenza dead bird surveillance and swabbing

Walk fixed sections during avian influenza outbreaks, report dead wild birds to the national helpline and, where you are trained and permitted, take oropharyngeal and cloacal swabs for testing. Wild bird surveillance is how new strains are detected.

Answers
Is it here?, How is it spread across the area?
Effort
Fixed beach or wetland sections walked weekly during outbreaks, each dead bird logged; swabbing only by trained staff, 5 minutes per bird
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Reporting needs no licence. Collecting carcasses and swabbing wild birds is done under the national surveillance programme (APHA in the UK, USDA APHIS and USGS National Wildlife Health Center in the US) with training and PPE; do not collect protected species without that cover.
How to run it
  1. Report before you touch: in the UK call the Defra helpline (03459 33 55 77) or use the online form for 1 or more dead birds of prey, 3 or more dead gulls or wild waterfowl (swans, geese, ducks), or 5 or more dead wild birds of any species; in the US report to the state wildlife agency or USDA Wildlife Services.
  2. Wear disposable gloves, an FFP2 or N95 mask and eye protection, and keep dogs and other people away from carcasses.
  3. If swabbing under a programme, take one oropharyngeal and one cloacal swab, place each in viral transport medium, label with the bird ID, and keep cold.
  4. Double bag any carcass you are asked to collect, disinfect boots and equipment with an approved disinfectant before leaving the site, and do not visit poultry or other bird sites the same day.
  5. Log species, location, count and condition for every carcass even when not collected, and keep the helpline reference number.
Kit list

The day one mistake: Picking up a dead swan bare handed to see what killed it. You risk infection and you have moved a carcass that surveillance staff needed in place; report it, photograph it, and leave it.

Bats

Bat radio tracking to roosts (VHF)

Attach a 0.3 to 0.5 g VHF transmitter to a bat caught under licence, then find its day roosts and foraging areas with a receiver and Yagi antenna. The only method that finds tree roosts and commuting routes reliably.

Answers
What is it doing?, How is it spread across the area?, Where does it occur across sites?
Effort
1 to 8 tagged bats per session, tag life 7 to 14 days, tracked to the day roost each morning and followed with 2 to 3 teams by night, fixes every 5 to 15 minutes by triangulation
Season
May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Funded project
Licence
Catching and tagging bats needs a licence in both countries (Natural England, NatureScot or NRW licence in the UK; USFWS permit for listed species plus state permits in the US). Gluing an external tag is not an ASPA regulated procedure, but the tag must stay under 5 per cent of body mass. Rabies vaccination for handlers is standard.
How to run it
  1. Under the licence, glue the tag between the shoulder blades of a bat above the weight threshold (tag no more than 5 per cent of body mass) and release it at the capture point.
  2. Search for the signal each morning from high ground or roads, then home in by walking towards the strongest signal with the gain turned down until you find the roost tree or building.
  3. At night station 2 or 3 teams at known points, take simultaneous bearings on a synchronised time signal every 5 to 15 minutes and triangulate the fix later.
  4. Record signal strength, bearing, time and your own GPS for every fix, and note when the signal is lost.
  5. Confirm each roost with an emergence count on a following evening and stop tracking when the tag drops off or falls silent.
Kit list
  • VHF receiver and Yagi antenna (Lotek, ATS, Wildlife Materials or Biotrack)
  • Radio tags (Lotek, Holohil or ATS)
  • Sighting compass (Suunto or Silva, via an outdoor shop)
  • Handheld GPS
  • Headlamp with red light
  • Skin adhesive for tags (Perma Type or Torbot Skin Bond via Lotek or NHBS (UK))

The day one mistake: Turning the gain up to hear the signal and then walking in circles. Signal bounces off buildings and hillsides at high gain; reduce gain until the signal is barely audible and the direction becomes clear.

Bats

Infrared and thermal camera emergence surveys (BCT 2023)

Record emergence with infrared or thermal cameras so that every bat leaving a roost is captured and can be counted on screen, as required by the BCT Bat Surveys for Professional Ecologists Good Practice Guidelines 4th edition (2023). Cameras replace or supplement human counters for complex or large roosts.

Answers
How many?, Is it here?, What is it doing?
Effort
1 camera per emergence point plus a backup, recording from 15 minutes before sunset to 90 minutes after (or 2 hours before sunrise for re entry), 2 to 3 surveys per roost, footage reviewed at up to 4x speed
Season
May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
Filming from outside a roost needs no licence in either country. Entering the roost or disturbing the bats needs a licence (Natural England class licence in the UK; state permits in the US).
How to run it
  1. Set the camera at least 5 m from the emergence point with the whole feature and some sky in frame, and add an infrared illuminator if using a near infrared camera.
  2. Start recording 15 minutes before sunset and stop 90 minutes after, or 2 hours before sunrise to 15 minutes after for re entry, on nights above 10 C without heavy rain.
  3. Run a bat detector recording next to the camera with a synchronised clock so calls can be matched to bats on screen.
  4. Review the whole recording, not just the first hour, and log every emergence and return with a time stamp and exit point.
  5. Store footage with the site, date and camera ID, and keep it for the report because the reviewer will ask to see it.
Kit list

The day one mistake: Pointing a thermal camera at a warm brick wall at dusk. Bats vanish against a background warmer than they are; frame the exit against sky or cool foliage or you will count nothing.

Bats

Bat box monitoring

Check numbered bat boxes on a monthly schedule for bats, droppings and other occupants. Boxes tell you which species use a site and, over years, whether the population is stable.

Answers
Is it here?, How many?, What is it doing?
Effort
10 to 30 boxes per site in groups of 3 facing different directions, checked monthly from April to October, under 2 minutes per box, checks only by a licensed bat worker
Season
Apr, May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
In the UK opening a bat box that may contain bats needs a Natural England, NatureScot or NRW bat licence; unlicensed volunteers may only watch. In the US no federal licence is needed for common species but state permits apply and any handling of listed species (for example Indiana bat) needs a USFWS permit.
How to run it
  1. Mount boxes at 3 to 5 m in groups of 3 on the same tree facing south, southeast and southwest, clear of branches beneath, and number and map them.
  2. Check monthly between April and October, in the middle of the day when bats are torpid, with a licensed bat worker doing any box opening.
  3. Look up into the box with a torch first; if bats are present, count and identify without handling unless the licence allows it.
  4. Record droppings, birds, wasps or dormice, and clean out bird nests after the season if the box is for bats.
  5. Log occupancy per box per visit and report any new species to the local bat group and the national recorder.
Kit list

The day one mistake: Checking boxes on a cold morning. Torpid bats in a box cannot fly and a cold check can leave them exposed all day; check in the warm part of the day and close the box within a minute.

Bats

Autumn swarming surveys

Survey caves, mines, tunnels and cellars in late summer and autumn when bats gather at the entrance to mate before hibernation. Swarming sites can draw bats from 60 km away and are protected as underground hibernacula in the UK.

Answers
Is it here?, What is it doing?, How many?
Effort
1 to 3 nights per site between mid August and October, detector and observer at the entrance from sunset to at least 2 am, calm dry nights
Season
Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
Watching from outside needs no licence in either country. Trapping at a swarming site needs a Natural England or NatureScot licence in the UK and state permits in the US, and entry to mines needs the owner's permission and a mines safety assessment.
How to run it
  1. Identify candidate sites from hibernation records and old mine plans, and get permission and a safety check on the entrance.
  2. Set up 10 to 20 m from the entrance from sunset with a full spectrum detector recording continuously and an observer with a red light.
  3. Count passes in and out at the entrance in 10 minute blocks, and note chasing, social calls and any landings on the rock.
  4. Stay until at least 2 am because Myotis swarming activity often peaks after midnight.
  5. If trapping is part of a licensed study, use a harp trap at the entrance, process bats within 30 minutes and release them at the trap.
Kit list

The day one mistake: Going home at midnight. Many Myotis swarm hardest between midnight and 3 am, and a survey that ends early records a quiet site that is actually busy.

Bats

Driven bat transects (NBMP car survey, iBats)

Drive a fixed route at low speed with a bat detector held out of the window recording continuously, so that bat calls can be identified and mapped afterwards. The NBMP Car Survey in the UK and iBats internationally cover far more ground per night than walking.

Answers
How is it spread across the area?, How many?, Is it here?
Effort
1 route of about 40 km driven at 15 mph (24 km per hour) starting 45 minutes after sunset, 2 surveys in July, detector on time expansion or full spectrum recording continuously
Season
Jul (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None for detector surveys. The driver holds nothing and the passenger runs the detector; tell the police if you are driving slowly on public roads at night.
How to run it
  1. Plan a route of about 40 km on quiet roads that avoids main roads and towns, and record it on GPS.
  2. Start 45 minutes after sunset on a dry night above 10 C, with the detector microphone held out of the passenger window pointing up at 45 degrees.
  3. Drive at a steady 15 mph (24 km per hour) with hazard lights on and record continuously with a GPS track log running.
  4. Repeat the same route on a second night in July, ideally within 2 weeks.
  5. Upload recordings and the GPS track to the scheme, then check automated identifications against the sonograms.
Kit list

The day one mistake: Driving at normal speed. Above 15 mph most calls are too brief to identify and the pass rate cannot be compared with any other survey; keep to the protocol speed and accept that the route takes 1.5 hours.

Bats

Dropping collection for DNA species identification

Collect fresh bat droppings from roosts and send them to a lab that amplifies mitochondrial DNA to identify the species. This tells you which species uses a roost without catching or disturbing a single bat.

Answers
Is it here?, Where does it occur across sites?
Effort
5 to 10 fresh droppings per site placed in a dry tube, 1 to 3 sites per survey visit, sent to a lab in batches, results in 2 to 4 weeks
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
Collecting droppings from outside a roost needs no licence. Entering a known roost to collect them disturbs bats and needs a Natural England, NatureScot or NRW licence in the UK; in the US check state permits, especially for listed species.
How to run it
  1. Search the roost, loft floor or wall below the entrance by day for droppings that are dark, crumbly and fresh looking, and photograph them in place.
  2. Pick up 5 to 10 droppings with clean tweezers or a fresh gloved hand into a labelled dry tube, one tube per pile.
  3. Keep tubes dry and cool, avoid touching the droppings with your skin or breathing on them, and post to the lab within a week or freeze them.
  4. Fill in the lab form with the site, date, structure and where in the roost each sample was taken.
  5. Record the lab result against the roost and treat a failed sample (no DNA) as no result rather than no bats.
Kit list

The day one mistake: Mixing droppings from several piles into one tube. A loft may hold two species and the lab returns one name, so keep every pile separate and note where it came from.

Small mammals

Camera trap tunnels and Mostela boxes

A wooden box with a plastic tunnel inside and a camera trap facing the tunnel, so that stoats, weasels and small mammals are filmed as they investigate. The Mostela design from the Dutch Small Mustelid Foundation is now the standard for small mustelid occupancy.

Answers
Is it here?, Where does it occur across sites?, What is it doing?
Effort
5 to 10 boxes per site 100 to 200 m apart along hedges and ditches, camera set to video, deployed for 14 to 28 days, checked weekly
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
None in the UK or the US; the animals are not caught. Landowner permission for boxes on private land.
How to run it
  1. Build or buy a box about 40 cm long with an 8 cm tunnel and a camera fixed at the far end, focused on the tunnel at 30 to 40 cm.
  2. Place boxes along linear features (hedge bases, ditches, walls, stone piles) 100 to 200 m apart, entrance low and level with the ground, and hide with vegetation.
  3. Set the camera to 10 to 20 second videos with a short delay, and check that the lens is clean and the infrared is not flaring on the tunnel.
  4. Leave for 14 to 28 days, check weekly for battery, card and damp, and swap cards without moving the box.
  5. Score every video for species, time and behaviour, and treat the first detection date as the key output for occupancy.
Kit list

The day one mistake: Setting the camera on still photographs. A weasel crosses the tunnel in under a second and the camera fires after it has gone; use video with a fast trigger and check the first week of footage before trusting the setup.

Small mammals

Squirrel drey counts and feeding sign transects

Count squirrel dreys along winter transects and score cone and nut feeding remains to estimate relative density and, from the cone and nut damage, which species is present. Cheap and repeatable; the drey index tracks red and grey squirrel numbers between years.

Answers
How many?, How is it spread across the area?, Is it here?
Effort
Transects 1 km long and 50 m wide (25 m each side) walked in leaf off months, dreys per hectare; feeding sign quadrats or 50 m strips every 200 m
Season
Nov, Dec, Jan, Feb, Mar (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None for counting dreys and signs. Red squirrel is protected in the UK (Schedule 5) so do not disturb dreys; in the US no permit is needed for sign surveys.
How to run it
  1. Lay out 1 km transects through woodland and walk them between November and March when the canopy is bare, scanning 25 m each side for dreys.
  2. Record every drey with GPS, tree species, height and whether it looks used (fresh leaves, intact) or old, and compute dreys per hectare (1 km by 50 m equals 5 ha).
  3. Every 200 m search a 50 m strip or a 5 by 5 m quadrat for stripped cones, split hazelnuts and bark strips, and count them.
  4. Examine cones and nuts: reds leave neat spirals and nuts split in two, greys chew more raggedly, but confirm species with a hair tube or camera.
  5. Repeat the same transects each winter and report the drey index and feeding sign counts together.
Kit list

The day one mistake: Counting dreys in summer. Leaves hide most of them and the count drops by half, so the index only works in the leaf off months on the same transects every year.

Medium and large mammals

Pine marten scat transects and den box checks

Walk forest tracks for pine marten scats, which are deposited on paths as territorial marks, and check den boxes for use. Scat DNA confirms the species and identifies individuals; den boxes show breeding.

Answers
Is it here?, Where does it occur across sites?, How is it spread across the area?
Effort
Transects of 1 to 2 km along forest tracks walked monthly, scats removed after recording; 10 to 20 den boxes per site checked 2 to 4 times per year
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Scat surveys need no licence. Pine marten is protected in the UK (Schedule 5) so disturbing a den needs a Natural England or NatureScot licence; American marten is a furbearer in the US and den checks need state permits.
How to run it
  1. Lay out 1 to 2 km transects along forest tracks and rides, which martens use for scent marking, and walk them monthly.
  2. Record every scat with GPS, freshness, size and contents, photograph it, then remove it so it is not counted next month.
  3. Bag fresh scats in tubes for DNA confirmation (fox and marten scats overlap in size) and keep them cool.
  4. Check den boxes from a ladder 2 to 4 times a year, in late winter for occupancy and in early summer for kits, using an endoscope so the box is not opened.
  5. Report scats per km and den box occupancy to the Vincent Wildlife Trust or the Mammal Society in the UK, or the state agency in the US.
Kit list

The day one mistake: Identifying every twisted scat as marten. Fox scats look the same and DNA shows up to a third of field identified marten scats are fox; collect samples and get the species confirmed before mapping.

Small mammals

Harvest mouse nest searches

Search tall tussocky grass, reed and bramble for the woven grass nests of harvest mice, which are visible after the vegetation dies back. The Mammal Society National Harvest Mouse Survey uses timed searches to map distribution.

Answers
Is it here?, How is it spread across the area?, Where does it occur across sites?
Effort
Searches of 30 to 60 minutes per 100 m of tall grass edge, in October to March after the vegetation has died back, 2 surveyors, nests per 100 m or per hour
Season
Oct, Nov, Dec, Jan, Feb, Mar (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None in the UK; harvest mouse is a priority species but nest searches do not disturb animals. No US equivalent; the species does not occur there.
How to run it
  1. Pick a search area of tall grass, reed, rush or bramble edge and walk it slowly parting the stems at 30 to 100 cm height where nests are woven.
  2. Search for 30 to 60 minutes per 100 m, or for a fixed time, with two people working the same strip from opposite sides.
  3. Record every nest with GPS, height above ground, plant species it is woven into, diameter and whether it is a breeding nest (over 6 cm, lined) or a smaller resting nest.
  4. Leave nests in place and do not open them; harvest mice may still be using them in mild winters.
  5. Record zero searches too, with the time spent, and submit all results to the national survey.
Kit list

The day one mistake: Searching in summer when the grass is green. Nests are invisible until the vegetation dies back in October, and searches before then record absence where there are mice.

Small mammals

Hedgehog torch and thermal transects

Walk a fixed route after dark with a spotlight or thermal camera and count every hedgehog seen, giving an encounter rate per km. With distance to each animal the counts can be turned into density by distance sampling.

Answers
How many?, Is it here?, How is it spread across the area?
Effort
Transects of 1 to 3 km walked at 2 km per hour, starting 1 hour after sunset, 3 to 5 nights per site, spotlight or thermal camera sweeping 20 m each side
Season
Apr, May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None for observation in the UK; marking or tagging hedgehogs needs no licence but should follow BHPS welfare guidance. Hedgehogs are not native to the US; no permit issue.
How to run it
  1. Design a route of 1 to 3 km along paths, lawns, field edges and hedges, and record it on GPS.
  2. Start 1 hour after sunset on nights above 8 C without heavy rain, walking at 2 km per hour sweeping a spotlight or thermal camera slowly 20 m each side.
  3. For each hedgehog record time, GPS, perpendicular distance, habitat and behaviour; do not pick it up unless marking under a study.
  4. Repeat each route 3 to 5 nights in the season, same start time.
  5. Report hedgehogs per km and submit sightings to the Big Hedgehog Map (PTES and BHPS).
Kit list

The day one mistake: Using a weak head torch and only looking at the path. Hedgehogs sit still in short grass 15 m away and are missed unless the beam sweeps slowly; use a bright spotlight or thermal and walk slowly.

Small mammals

Invasive rodent tracking tunnels, chew cards and wax tags (island eradication standard)

Ink tracking tunnels, chew cards and wax tags detect rats and mice at very low density, which is what island eradication projects and seabird island biosecurity need. Protocols follow the New Zealand Department of Conservation and the RSPB and Island Conservation eradication standards.

Answers
Is it here?, Where does it occur across sites?
Effort
Devices on a 50 m grid or every 50 m along lines (25 m for mice), tracking tunnels 1 night, chew cards and wax tags 7 nights, checked quarterly in biosecurity and every night during an eradication
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
Detection devices need no licence in the UK or the US. Any subsequent poisoning or trapping is regulated (HSE approved rodenticides and CRRU rules in the UK; EPA registered products and state permits in the US) and needs landowner and conservation body approval on island projects.
How to run it
  1. Lay a grid of devices at 50 m spacing for rats and 25 m for mice, numbered, mapped and flagged, covering the whole island or the area under check.
  2. Bait tracking tunnels with peanut butter on a fresh ink card, set chew cards (corflute with peanut butter in the flutes) and wax tags at ground level, and leave tracking tunnels 1 night and chew cards and wax tags 7 nights.
  3. Collect cards and tags, and identify prints and bite marks with a reference key: rat incisor marks are 2 mm wide and paired, mouse marks are under 1 mm.
  4. In a biosecurity check every quarter run the whole grid and report any sign the same day; during an eradication check every night and mark every detection.
  5. Store used cards for review and keep a detection map so that a single rat can be targeted.
Kit list
  • Ink tracking tunnel (NHBS (UK) or Forestry Suppliers (US))
  • Chew cards and wax tags (Pest Control Research (NZ), or make from corflute and paraffin wax)
  • Peanut butter
  • Flagging tape
  • Handheld GPS
  • Ink tracking tunnels and cards (Gotcha Traps or Connovation (NZ), or make from corflute)

The day one mistake: Reading a smudged print as no rat. A single faint set of prints on one card is an incursion on an eradicated island; escalate every uncertain sign, photograph it, and re set the device with fresh bait.

Small mammals

Live pitfall trapping for shrews

Bury open topped containers flush with the ground so shrews fall in and are held alive with food and bedding. The only cheap method that catches shrews reliably, but shrews starve within hours so the check interval is the whole method.

Answers
Is it here?, How many?
Effort
10 to 20 pitfalls per line at 5 m spacing, opened 2 to 3 nights, checked every 2 hours by day and every 3 to 4 hours at night, with food and bedding in every trap
Season
Apr, May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
In the UK any trap that may catch shrews (Wildlife and Countryside Act Schedule 6) needs a licence: in England the Natural England general licence GL01 applies without application provided its food and check conditions are met; in Scotland and Wales an individual NatureScot or NRW licence is needed. In the US state scientific collecting permits apply.
How to run it
  1. Dig in 10 to 20 buckets or 2 litre tubs at 5 m spacing along a line, rim flush with the soil, with drainage holes and a rain cover 5 cm above.
  2. Put in dry bedding and a generous supply of food (blowfly pupae or mealworms, plus a little wet dog food) in every trap every time it is set.
  3. Open in the evening and check every 2 hours by day and every 3 to 4 hours at night, without exception, in the cool part of the year (not above 25 C).
  4. Identify, weigh and release each shrew at the trap within 2 minutes, and refresh food and bedding.
  5. Close traps by day if you cannot check them and remove all containers at the end of the session.
Kit list

The day one mistake: Leaving the traps overnight to check at dawn. A shrew must eat every 2 to 3 hours and every trap you find in the morning will hold a dead one; if you cannot check through the night, close the traps at dusk.

Medium and large mammals

Mink rafts

A floating raft with a clay pad under a tunnel records the footprints of mink passing along the bank, and the same raft carries a live trap once mink are confirmed. The GWCT mink raft is the basis of every UK mink control project and of the North American Mink control programmes.

Answers
Is it here?, Where does it occur across sites?, How is it spread across the area?
Effort
1 raft per 1 km of bank, checked every 14 days for prints, all year, 2 minutes per check; rafts switched to trap mode once mink prints appear
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Monitoring mode needs no licence. Trapping needs no licence in England but you must check traps daily, use an approved trap, and dispatch mink humanely; in Scotland a Spring Traps Approval Order or a licence applies. In the US mink is a furbearer, so state trapping licences and seasons apply, and any live trapping outside the season needs a state permit.
How to run it
  1. Build or buy a raft with a 10 cm tunnel over a basket of clay and oasis foam, and tether it to a bank under cover every 1 km of watercourse.
  2. Wet the clay so it takes a print, then check every 14 days in monitoring mode, smoothing the pad after each check.
  3. Identify prints with a key: mink show 5 toes in a star shape about 3 cm across, water vole and rat are smaller, otter much larger.
  4. When mink prints appear, replace the clay basket with a live cage trap inside the tunnel and check the trap at least once every 24 hours, ideally at dawn.
  5. Dispatch trapped mink humanely under the law, release all non target animals at once, and return the raft to monitoring mode.
Kit list

The day one mistake: Leaving a trap set through a weekend. A water vole or a polecat in an unchecked cage dies, and the project loses its landowner; if you cannot check daily, take the trap out and leave the clay in.

Marine mammals and seabirds

Marine mammal observer and PAM mitigation watches (JNCC protocol)

Trained marine mammal observers and passive acoustic monitoring operators watch a mitigation zone around seismic, piling or explosive work and delay operations if a marine mammal is inside it. The JNCC guidelines for minimising the risk of injury to marine mammals set the UK protocol; NMFS incidental take authorisations set the US one.

Answers
Is it here?, What is it doing?, How is it spread across the area?
Effort
Pre watch of 30 minutes (60 minutes in water deeper than 200 m) before any noise source starts, continuous watch during operations, 500 m mitigation zone, 20 minute soft start, PAM operator on hydrophones at night and in poor visibility
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Funded project
Licence
In the UK MMOs must have completed a JNCC recognised course and the operation runs under a Marine Licence or petroleum consent. In the US observers are Protected Species Observers approved by NMFS under the project's Incidental Harassment Authorisation or Letter of Authorisation.
How to run it
  1. Before the source starts, watch the full 500 m mitigation zone for 30 minutes (60 minutes in water over 200 m) from the highest safe platform, with a second observer or a PAM system when visibility is poor.
  2. Record every sighting with time, bearing, distance by reticle binoculars, species, number, behaviour and heading, and whether it was inside the zone.
  3. If an animal is inside the zone, tell the operator to delay the start until 20 minutes have passed since the animal was last seen leaving.
  4. Ensure a soft start (gradual ramp up) of at least 20 minutes and log the start, stop and any breaks in the source.
  5. Complete the JNCC marine mammal recording forms (or the NMFS report) with effort, environmental conditions and every mitigation action, and submit them within the required period.
Kit list
  • Reticle binoculars 7×50 (Steiner or Fujinon, via an optics dealer)
  • PAM hydrophone system (Seiche Ltd or Vanishing Point (PAMGuard software is free))
  • Handheld GPS
  • Sighting compass (Suunto or Silva, via an outdoor shop)
  • Waterproof notebook

The day one mistake: Starting the pre watch late so the source is not delayed. If the 30 minutes are not complete and logged before the soft start, the mitigation is invalid and the whole operation can be stopped by the regulator; the watch starts when you say it does, not when the crew is ready.

Marine mammals and seabirds

Seabird productivity plots and chick provisioning watches

Mark nests on cliff plots and follow them to fledging to measure chicks fledged per pair, and watch nests to record how often adults bring food and what they bring. Productivity is the earliest warning of a food shortage at sea and the JNCC Seabird Monitoring Programme method is used on both sides of the Atlantic.

Answers
What is it doing?, How many?
Effort
Plots of 20 to 50 nests per species per colony checked every 3 to 5 days from incubation to fledging (JNCC Seabird Monitoring Handbook); provisioning watches of 4 to 6 hours or dawn to dusk on 5 to 10 nests, 3 to 5 watches per season
Season
May, Jun, Jul, Aug (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Watching from outside a colony needs no licence in either country. Entering a colony to mark nests or ring chicks needs a BTO permit and landowner consent in the UK, and a federal banding permit and refuge access permission in the US.
How to run it
  1. Choose plots visible from a safe vantage point, photograph them, and mark 20 to 50 nests per species on the photo with numbers before eggs hatch.
  2. Visit every 3 to 5 days from late May, recording each nest as egg, small chick, large chick, empty or failed, and note the date of first sighting of a chick.
  3. Continue until chicks fledge (kittiwake at about 40 days, guillemot jumps at 20 days) and score the plot as chicks fledged per active nest.
  4. For provisioning, watch 5 to 10 nests for 4 to 6 hours or dawn to dusk from a hide, logging every feed with time, nest, prey type and size relative to bill length.
  5. Repeat provisioning watches 3 to 5 times through chick rearing and report both productivity and feeds per chick per hour to the SMP or the state coordinator.
Kit list

The day one mistake: Setting up the plot after chicks have hatched. Nests that failed at the egg stage are missed and productivity comes out too high; mark nests during incubation and count failures as zero.

Marine mammals and seabirds

Burrow nesting seabird playback surveys (shearwaters and storm petrels)

Play the species' call at burrow entrances by day and count the burrows that reply, then convert to apparently occupied burrows using a calibration of the response rate. The JNCC method for Manx shearwater and storm petrel is the only way to census birds that are underground by day and at sea by night; puffin burrows are scored from signs, not playback.

Answers
How many?, Is it here?, How is it spread across the area?
Effort
Burrows sampled in 1 m or 2 m quadrats or on transects, playback of 10 seconds at each burrow entrance with a 10 second listen, repeated once, 3 visits per site, daytime in early to mid incubation, response rate calibrated on 100 burrows
Season
May, Jun, Jul (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
None for playback at burrows in the UK if no bird is handled, but most colonies are SSSIs or reserves that need consent and rat free islands need biosecurity checks on your kit. In the US playback at burrows of listed species (for example Hawaiian petrel) needs a USFWS permit.
How to run it
  1. Map the colony and lay out random quadrats or transect strips, then count every burrow entrance within them, marking each with a numbered cane.
  2. Between late May and early July, play the call at each burrow entrance for 10 seconds with a speaker at the entrance, listen 10 seconds, and repeat once.
  3. Record a response, no response or unknown, and note burrows that are collapsed or obviously unused.
  4. Calibrate the response rate on at least 100 burrows by playing to each on 3 or more days in the same season, because a bird responds on only some visits (about 50 to 60 per cent for Manx shearwater).
  5. Divide the number of responding burrows by the response rate to give apparently occupied burrows, multiply by burrow density and colony area, and submit to the Seabird Monitoring Programme.
Kit list

The day one mistake: Skipping the calibration and reporting responding burrows as pairs. Half the birds do not answer on any one day, so the raw count underestimates the colony by half or more; the calibration is not optional.

Marine mammals and seabirds

Beached bird surveys with oiling scores

Walk fixed beach sections and record every dead seabird with its species, age and whether it is oiled, giving corpses per km and an oil rate. The RSPB Beached Bird Survey (since 1971), the North Sea international survey and COASST in the US use this to track chronic oil pollution and mass mortality events.

Answers
How many?, Is it here?, How is it spread across the area?
Effort
Fixed beach sections of 2 to 5 km walked once a month at low tide, monthly or in the February international count, corpses per km with an oiling score, 1 to 3 hours per section
Season
Jan, Feb, Mar, Oct, Nov, Dec (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None for counting corpses. Collecting corpses of protected species for a museum or a lab needs a Natural England general licence in the UK or a USFWS salvage permit in the US, and during avian influenza outbreaks corpses should be reported and not handled.
How to run it
  1. Register a fixed beach section of 2 to 5 km and walk the full length along the strandline once a month, or at least in the February count, at or near low water.
  2. For every corpse record species, age (juvenile or adult), condition, and whether it is oiled, scoring the fraction of the body oiled (none, under 25 per cent, 25 to 50, 50 to 75, over 75 per cent).
  3. Photograph the corpse with a scale, measure wing chord if the species is uncertain, and use COASST style foot and wing keys for partial remains.
  4. Mark each corpse (cut a wing tip or spray paint) so it is not recounted next month, and remove it above the strandline if the scheme asks.
  5. Wear gloves, wash hands, report any large mortality to the scheme and to the avian influenza helpline, and submit the count as corpses per km with the oiling rate.
Kit list

The day one mistake: Walking the section at high water when the strandline is under water and half the corpses are floating. Survey at low water along the whole strandline, or the count reflects the tide rather than the birds.

Medium and large mammals

VHF radio tracking and triangulation

Fit an animal with a VHF transmitter and locate it by taking compass bearings to the strongest signal from two or more known points. Gives home range, habitat use and survival at a fraction of the cost of GPS collars.

Answers
What is it doing?, How is it spread across the area?, Is it here?
Effort
3 or more bearings per fix taken within 10 minutes from stations 200 to 500 m apart, 1 fix per animal per 4 to 6 h, 10 to 20 animals, 6 to 12 months
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Funded project
Licence
Collaring is a regulated procedure. UK: Home Office project and personal licences under ASPA plus a Natural England or NatureScot licence for protected species. US: IACUC approval plus a state scientific collecting permit, and a USFWS permit for listed species.
How to run it
  1. Fit collars at under 3 percent of body mass under the project licence and let the animal settle for 7 days before the first fix counts.
  2. Choose fixed tracking stations with clear sky lines and record their GPS positions to the metre.
  3. At each station rotate the antenna slowly, find the null or peak, read the compass bearing and note the time and signal strength.
  4. Take 3 bearings from different stations within 10 minutes so the animal has not moved; angles of 30 to 150 degrees between bearings give the smallest error polygon.
  5. Enter fixes into LOAS or similar software the same day and check every error polygon over 1 ha before it enters the analysis.
Kit list
  • VHF receiver and Yagi antenna (Lotek, ATS, Wildlife Materials or Biotrack)
  • VHF transmitter collars (Lotek, ATS or Biotrack)
  • Sighting compass (Suunto or Silva, via an outdoor shop)
  • Handheld GPS
  • Headlamp

The day one mistake: Taking the three bearings 30 minutes apart. The animal walks 300 m between them, the lines never cross cleanly and every fix is thrown out. Use radios so all observers take bearings on the same minute.

Medium and large mammals

Roadkill surveys

Record every dead animal on a fixed stretch of road to map collision hotspots and track relative abundance of species such as hedgehogs, badgers, otters and deer. The Mammal Society and Project Splatter run national schemes.

Answers
How is it spread across the area?, Is it here?, How many?
Effort
Fixed routes of 10 to 50 km driven at 30 to 50 km per h, or 2 to 5 km walked, once a week to once a month, early morning before scavengers and traffic remove carcasses
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
No licence to record. Stopping on motorways and interstates is illegal; survey only roads where you can pull off safely. Moving a carcass of a protected species for study may need a licence in the UK; in the US, possession of migratory bird or listed species remains is an offence.
How to run it
  1. Choose a fixed route and drive or walk it in the same direction at the same time of day, with a passenger recording so the driver never records.
  2. Log every carcass with GPS, species, age class if obvious and a photograph; record unidentifiable remains as unknown mammal rather than guessing.
  3. Note the road side, verge type, adjacent habitat and any fence or wall within 10 m of the carcass.
  4. Record zero surveys in full; a route with no carcasses is a data point, not a wasted trip.
  5. Upload to the national scheme (Project Splatter in the UK, state roadkill portals such as CROS in California) and keep your own copy with route length as the denominator.
Kit list

The day one mistake: Surveying at midday. Crows, foxes and traffic strip small carcasses within hours, so afternoon counts miss most hedgehogs and amphibians. Start at first light and be consistent.

Medium and large mammals

Thermal imaging transects for deer, hares and nocturnal mammals

Walk or drive fixed lines at night with a thermal camera and record every warm body with its distance and bearing. Detects deer and hares in cover that spotlighting misses and does not disturb the animals.

Answers
How many?, How is it spread across the area?, Is it here?
Effort
Transects of 1 to 3 km walked or driven at 2 to 3 km per h, first 4 h after dusk, 2 to 3 repeats, sightings recorded with distance and angle for distance sampling
Season
Oct, Nov, Dec, Jan, Feb, Mar (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
None for observation. Landowner permission for night access is essential and tell the local police or estate if you will be on roads with a camera at night. Thermal counts near a deer cull are not a survey.
How to run it
  1. Plan straight transects that cross the habitat and mark GPS start and end points in daylight.
  2. Begin 1 h after sunset on clear nights below 10 C when the contrast between animals and ground is greatest.
  3. Scan a slow arc ahead and to both sides every 20 to 30 m, identify each animal with binoculars or a zoom in the thermal image, and record species and group size.
  4. Measure distance with a laser rangefinder and bearing with a compass at first detection, never after the animal moves.
  5. Repeat each transect 2 to 3 times over a fortnight and analyse in Distance software with a minimum of 60 to 80 detections per species.
Kit list

The day one mistake: Counting the same herd twice after it runs ahead of you along the line. Record group position and heading and drop any group that reappears within 200 m in the direction of travel.

Medium and large mammals

Scent stations and track plates

A smoothed disc of sand or a sooted metal plate with a scent lure in the middle records the footprints of carnivores that visit overnight. The USFWS scent station index for foxes, coyotes and bobcats is the standard design.

Answers
Is it here?, Where does it occur across sites?, How is it spread across the area?
Effort
10 to 30 stations 300 m to 1 km apart, 3 to 5 consecutive nights, checked every morning, repeated in 2 seasons
Season
Apr, May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None in the UK or US for a non capture attractant, but landowner permission is needed and lures must not be used where they could draw animals onto roads. Check state rules on carnivore attractants during hunting seasons.
How to run it
  1. Rake a 1 m circle of sieved sand or fine soil to a smooth surface, or lay a 40 by 80 cm aluminium track plate coated in soot or chalk and carbon inside a corrugated plastic tunnel.
  2. Place a fatty acid scent tablet or a few drops of lure on a stake in the centre and record GPS, habitat and date.
  3. Check every morning within 2 h of sunrise, photograph every track with a scale bar, then re smooth or replace the plate.
  4. Identify tracks with a field guide and record unclear prints as unknown; do not force a species onto a smudge.
  5. Run 3 to 5 nights, record station nights as the effort denominator, and rest the site for at least 4 weeks before repeating.
Kit list

The day one mistake: Using so much lure that animals roll on the station and destroy every print. One tablet or 3 drops of lure per station is enough; more gives you a mess instead of tracks.

Medium and large mammals

Deer browsing and impact assessment

Score browsing on seedlings, coppice regrowth and shrubs to measure the effect of deer on woodland structure. Follows the Forestry Commission Woodland Herbivore Impact Assessment and the AVID method (Assessing Vegetation Impacts from Deer, Cornell and New York DEC) used in North America.

Answers
How many?, How is it spread across the area?
Effort
10 to 20 plots of 5 m radius or 2 m by 20 m strips per stand, 200 m apart, 15 minutes each, once a year in late winter before leaf burst
Season
Feb, Mar, Apr (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None for the assessment. Landowner permission is needed in the UK and US and you will be walking through stands during the deer culling season, so agree dates with the stalker or hunting lease first.
How to run it
  1. Lay plots on a systematic grid and mark the centre with a stake and GPS so the plot can be relocated next year.
  2. In each plot record the height and browse status of every tree seedling and sapling under 2 m, counting the proportion of leading shoots browsed.
  3. Score ground flora and shrub layer on the 0 to 3 impact scale (none, light, moderate, heavy) using the indicator species and browse line height in the standard form.
  4. Note fraying, bark stripping, deer paths and dung groups so the impact can be attributed to deer rather than livestock or rabbits.
  5. Survey in February to April before bud burst, repeat annually on the same plots, and compare against fenced exclosure plots where they exist.
Kit list

The day one mistake: Scoring only the most obvious eaten patch beside the ride. Deer impact is patchy, so a fixed grid with plots in the dull interior of the stand is the only way to get a number you can compare next year.

Medium and large mammals

Behavioural sampling (focal follows, scan sampling, activity budgets, ethograms)

Watch animals in a structured way and record what they do using a written ethogram, so activity budgets and social interactions can be compared between sites or seasons. The Altmann 1974 sampling methods are still the standard.

Answers
What is it doing?, How many?
Effort
Focal follows of 10 to 30 minutes per animal or group scans every 5 to 15 minutes, 4 to 8 h per day, across the full daylight period and repeated over 10 to 30 days
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None for observation in the UK or US. Following animals in national parks or reserves needs a research permit, and habituating wild primates or carnivores raises welfare and safety issues that need ethical review.
How to run it
  1. Write an ethogram before day one: a list of mutually exclusive behaviours, each with a one sentence definition, and test it with a second observer until you agree on 90 percent of records.
  2. Choose the sampling rule: focal animal sampling for one individual, or scan sampling where you record what every visible animal is doing at a fixed instant.
  3. Set a timer and record at the beep, never in between; for focal follows note the start and end time and any minutes the animal was out of sight.
  4. Stay far enough away that the animal does not change behaviour because of you, and abandon a follow if it does.
  5. Balance observation time across morning, midday and evening and across individuals so the budget is not built on the tamest animal at the easiest hour.
Kit list

The day one mistake: Writing down what the animal is doing when you look up rather than at the beep. Recording the interesting moments and skipping the resting ones inflates active behaviours by half. The timer decides when you record.

Medium and large mammals

Snow tracking transects

Walk a fixed route after fresh snow and record every track crossing to give an index of lynx, marten, hare, fox and wolf abundance. The Finnish wildlife triangle scheme and the Minnesota carnivore track survey are the standard designs.

Answers
Is it here?, How is it spread across the area?, How many?
Effort
Transects of 2 to 10 km on foot, ski or snowmobile, surveyed 24 to 72 h after fresh snowfall, 2 to 5 repeats per winter
Season
Nov, Dec, Jan, Feb, Mar (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None to observe tracks. Access permission in the UK; in the US, check that winter closures in national forests and parks allow entry and that snowmobile use is legal on your route.
How to run it
  1. Wait 24 to 72 h after the snow stops so that animals have moved but tracks are still crisp; abandon the survey if it thaws or snows again.
  2. Walk the fixed line and record every set of tracks that crosses it, identifying species from print shape, gait and stride, and photographing anything uncertain with a ruler.
  3. Follow a track for 20 to 50 m off the line when needed to confirm species or the number of animals, then return to the same point.
  4. Record snow depth, snow age in hours and whether the surface is powder or crust, because these change detection more than animal numbers do.
  5. Divide crossings by transect length and by days since snowfall to make counts comparable between visits.
Kit list

The day one mistake: Surveying the morning after a heavy overnight fall. Nothing has moved yet, you record zero and conclude the species is absent. One clear night of animal movement is the minimum before the survey counts.

Medium and large mammals

Wildlife crossing and underpass monitoring

Record which species use underpasses, green bridges, culverts and fence gaps and how often, to test whether mitigation on a road or railway works. Combines camera traps at each entrance with a raked track bed inside the tunnel.

Answers
What is it doing?, Is it here?, How many?
Effort
1 to 2 camera traps per structure plus a track bed, checked every 2 to 4 weeks, 12 months minimum, with control cameras 100 to 500 m from the structure
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
None for cameras, but you need permission from the highway or rail authority (National Highways or Network Rail in the UK, state DOT in the US) and a lineside safety briefing before entering any structure under a live road or railway.
How to run it
  1. Map every structure and fence end along the road and record dimensions, openness ratio and whether water or a dry ledge runs through.
  2. Mount a camera trap at each entrance at 40 to 60 cm height angled along the tunnel, set to 3 shot bursts with no delay, and secure it in a lockbox.
  3. Rake a 1 m wide bed of sand or marble dust across the full width inside the tunnel and check it every 2 to 4 weeks with the cameras.
  4. Score each visit as a complete crossing, a turn back or a pass by the entrance, because refusals are the key result for design.
  5. Place control cameras away from the structure to compare use with local abundance, and report crossings per camera night.
Kit list

The day one mistake: Pointing the camera straight into the tunnel mouth. Backlight blows out every image and the trigger fires on every passing car. Angle the camera 30 to 45 degrees along the wall inside the entrance.

Amphibians

Chytrid and ranavirus swabbing

Rub a sterile swab over the skin of each amphibian and send the swabs to a lab for qPCR detection of Batrachochytrium dendrobatidis, B. salamandrivorans or ranavirus. The only reliable way to know whether a disease is present at a site.

Answers
Is it here?, Where does it occur across sites?, How is it spread across the area?
Effort
30 animals per site per species for 95 percent confidence at 10 percent prevalence, one swab per animal, new gloves per animal, 1 to 2 visits per season
Season
Mar, Apr, May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
Handling requires a licence for protected species: Natural England, NatureScot or NRW licence for great crested newts and natterjacks in the UK; state scientific collecting permits in the US and a USFWS permit for listed species. Toe clipping is a regulated procedure under ASPA in the UK and needs IACUC approval in the US.
How to run it
  1. Order sterile rayon tipped swabs and screw cap tubes from the lab you will use and agree the storage method (dry, or in 70 percent ethanol) before you go.
  2. Catch each animal with a new pair of nitrile gloves or a new plastic bag, and never let two animals share a container.
  3. Swab 5 strokes on each side of the belly, each inner thigh and each hind foot for chytrid (30 strokes total); for ranavirus take an oral or cloacal swab, or a toe clip only under a licence.
  4. Snap the swab tip into its labelled tube, keep tubes cool, and freeze them at the end of the day.
  5. Disinfect boots, nets and containers with 1 percent Virkon or 70 percent ethanol between every site, and send swabs with a chain of custody sheet.
Kit list

The day one mistake: Reusing gloves between two frogs. One positive animal contaminates the next swab and the site is recorded as an outbreak that never happened. A new pair of gloves per animal is the whole point of the method.

Amphibians

Toad patrols and road crossing surveys

Volunteers walk a road crossing point on migration nights, count and carry toads, frogs and newts across, and record the dead. Froglife Toads on Roads registers crossings in the UK and the data show which sites need tunnels or road closures.

Answers
How many?, What is it doing?, How is it spread across the area?
Effort
Nightly patrols of a 200 m to 1 km road section from dusk for 2 to 3 h on mild wet nights above 5 C, for 3 to 6 weeks of the spring migration
Season
Feb, Mar, Apr (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
No licence to move common toads and frogs in the UK, but great crested newts must not be handled without a licence and are recorded and left. In the US, check state rules; many states allow moving common species but not listed ones. Road safety rules of the highway authority apply.
How to run it
  1. Register the crossing with Froglife (UK) or the local amphibian group and agree who is patrol coordinator and how the road authority is told.
  2. Patrol only on evenings above 5 C with rain or high humidity, wearing high visibility vests and a head torch, and start at dusk.
  3. Walk the road edge and verge in pairs, one on each side, picking up animals with wet hands or a bucket and carrying them in the direction they were heading.
  4. Count live animals by species and sex or pair, count the dead separately, and record the time and the weather.
  5. Release animals on the pond side at least 5 m beyond the road and never in the pond itself, and never drive a car along the crossing while patrolling.
Kit list

The day one mistake: Carrying toads to the nearest verge rather than the direction they were travelling. Half of them turn round and cross again. Look at which way the animal faces and take it to the pond side or the terrestrial side accordingly.

Amphibians

Mitigation capture and translocation with exclusion fencing

On development sites, amphibians and reptiles are fenced out, trapped and moved to a prepared receptor site before construction. The Natural England great crested newt and reptile mitigation guidelines set the effort and the fence specification.

Answers
How many?, Is it here?, How is it spread across the area?
Effort
Fence the whole site, pitfalls every 10 m and refugia every 5 to 10 m along the fence, checked daily within 4 h of dawn, until 5 consecutive clear days after a minimum of 30 days at over 5 C night temperature
Season
Mar, Apr, May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Funded project
Licence
UK: a Natural England, NatureScot or NRW mitigation licence for great crested newts, sand lizard and smooth snake; widespread reptiles follow Froglife and ARG UK guidance under the Wildlife and Countryside Act. US: state scientific collecting and relocation permits, and USFWS permits for listed species.
How to run it
  1. Install a 500 mm high polythene or Herpetosure fence buried 100 mm and sloped to the site, with a 1 m strip mown at the base, and inspect it daily for gaps.
  2. Sink pitfall buckets every 10 m along the inside of the fence and lay felt or tin refugia at 5 to 10 m intervals with a minimum of 100 per ha for reptiles.
  3. Check every trap and refugium daily within 4 h of dawn, with cover boards checked before the pitfalls, and never leave buckets open in heat or frost.
  4. Record each animal by species, sex and life stage, move it the same day to the receptor site, and release it under cover in the evening or in shade.
  5. Continue until 5 clear days with no animals after the minimum 30 trapping days (60 for medium or large populations), then destructive search the site by hand before the fence comes down.
Kit list

The day one mistake: Missing the daily check on a hot bank holiday. Newts and lizards die in dry buckets within a day and the licence is revoked. Two people are named on every day of the rota and the buckets have a damp sponge in each.

Amphibians

Terrestrial and stream visual encounter surveys

Search a fixed area or stream length for a fixed time, turning rocks, logs and leaf litter and replacing them, to record salamanders, frogs and newts on land. The USGS ARMI protocols and the Heyer et al. 1994 standard methods set the time constrained search rules.

Answers
Is it here?, How many?, Where does it occur across sites?
Effort
Plots of 10 by 10 m or stream reaches of 50 to 100 m, 1 to 2 person hours each, cover objects turned and replaced, 3 visits, damp days or nights above 8 C
Season
Mar, Apr, May, Jun, Sep, Oct, Nov (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
Handling great crested newts in the UK needs a Natural England, NatureScot or NRW licence; other species may be handled without one but not sold or harmed. US: state scientific collecting permits for handling, and a USFWS permit for listed salamanders such as Shenandoah or Cheat Mountain.
How to run it
  1. Mark the plot or reach with GPS and flag the corners so the same area is searched every visit.
  2. Search for a fixed time, turning every cover object you can lift safely and replacing it exactly, and rake leaf litter with your fingers in 1 m squares within the plot.
  3. Handle animals only with wet or gloved hands, hold them for under 2 minutes for identification and measurement, and release them under the same object.
  4. In streams, work upstream so silt does not cloud the water ahead of you, and check the underside of rocks in riffles as well as the bank edge.
  5. Record search time, number of searchers and cover objects turned, and disinfect boots and gloves between sites.
Kit list

The day one mistake: Leaving a log rolled over. The moist underside dries, the salamander below has lost its home and the next visit finds nothing. Roll it back exactly and put the animal beside it, not under it, so it is not crushed.

Amphibians

Larval dip netting and tadpole counts

Sweep a dip net through pond margins and count and identify the tadpoles and larvae caught to confirm breeding and compare larval abundance between ponds. It is the standard larval confirmation method in the NARRS scheme in the UK and in the USGS ARMI protocols in the US.

Answers
How many?, Is it here?, Where does it occur across sites?
Effort
15 to 20 sweeps of 1 m spaced round the whole pond margin, or 1 sweep per 2 m of shoreline, 2 to 3 visits from late spring, sorted in a white tray
Season
Apr, May, Jun, Jul, Aug (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: netting where great crested newts may be present needs a Natural England, NatureScot or NRW licence, since larvae are as protected as adults. US: state permits for collection and handling; most states allow catch and release of common tadpoles without one.
How to run it
  1. Walk the pond margin and space the sweeps evenly so every habitat type (open water, emergent plants, submerged weed) gets a share.
  2. Sweep the net 1 m through the water and vegetation, then tip it into a white tray of pond water and count each species and stage.
  3. Identify larvae with a hand lens using tail fin shape, eye position and gill form, and photograph anything uncertain rather than keeping it.
  4. Return all larvae to the pond within 10 minutes and never transfer water, larvae or weed between ponds.
  5. Record the number of sweeps as the effort denominator and repeat 2 to 3 times through the season to catch late developers.
Kit list

The day one mistake: Netting only at the easy access point on the one open bank. Tadpoles cluster in warm shallow weed and larvae hide under the far margin, so a single bank gives a count that changes with the wind, not the pond.

Amphibians

Automated frog call recorders

Leave an AudioMoth or Song Meter beside the water to record frog and toad choruses through the night, then score calling activity from the files. Extends the NAAMP call index across many sites without night visits.

Answers
Is it here?, Where does it occur across sites?, How is it spread across the area?
Effort
1 recorder per pond or wetland at 1 to 2 m from the water edge, 1 minute in every 10 from dusk to dawn, 2 to 6 weeks per season
Season
Feb, Mar, Apr, May, Jun, Jul, Aug (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
None for recording in the UK or US. Landowner permission for installing a unit on the site and a note to the manager so it is not removed.
How to run it
  1. Set the recorder to a duty cycle such as 1 minute in 10 from 1 h before sunset to 1 h after sunrise, with a sample rate of 32 kHz, and test it at home for one night.
  2. Fix the unit inside a waterproof case on a stake or tree 1 to 2 m from the water edge and 1 m above ground, microphone pointing at the pond.
  3. Log GPS, deployment time, card ID and a reference photo, and add a temperature logger beside it because calling depends on temperature.
  4. Retrieve after 2 to 6 weeks, back up the cards, and score each 1 minute file with the NAAMP index 0 to 3 (none, individuals countable, overlapping, full chorus).
  5. Verify every species by listening to at least 3 files and checking a spectrogram before it enters the site list.
Kit list

The day one mistake: Recording continuously at 48 kHz for six weeks. The card fills in a week and the batteries die in ten days, so the peak of the chorus is missed. A 1 minute in 10 duty cycle and 32 kHz captures every species and lasts the season.

Reptiles

Crocodilian spotlight eyeshine counts

Drive a boat along a fixed stretch at night and count the red eyeshine of crocodiles, alligators and caimans, estimating size class for each. Standard for the Florida alligator, Australian saltwater crocodile and IUCN Crocodile Specialist Group monitoring.

Answers
How many?, How is it spread across the area?, Is it here?
Effort
Boat transects of 5 to 20 km at 8 to 10 km per h starting 1 h after sunset, both banks scanned, 2 to 3 repeats on calm dark nights
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
Observation needs a state or territory research permit in the US (Florida FWC, Louisiana LDWF) and Australia, and boat licences and night navigation rules apply. Capture or marking is a separate permit. No UK species.
How to run it
  1. Run the transect in daylight first to map hazards, and record water level and tide state because counts fall when the water is high.
  2. Start 1 h after sunset with one spotter on the bow holding a 100,000 candlepower spotlight or a strong headlamp at eye level, and the driver keeping a steady 8 to 10 km per h.
  3. Sweep the beam slowly across the water and both banks, and when eyes are seen approach to within 10 to 20 m to estimate total length from the snout to eye distance.
  4. Record every animal with GPS, size class (hatchling, juvenile, subadult, adult) and eyes only if it submerged before sizing.
  5. Stop if wind exceeds 15 km per h or it rains, and calculate animals per km of transect for each repeat.
Kit list

The day one mistake: Shining the light from the driver's seat behind the spotter. The reflection only shows when the beam and your eye are on the same line, so the spotter holds the light beside their head and nobody else uses a torch.

Reptiles

Sea turtle in water surveys

Count sea turtles at sea from a boat or by snorkelling on reef flats and seagrass, and capture them by hand for tagging and measurement where permitted. Gives abundance and size structure of the foraging population that beach counts miss.

Answers
How many?, How is it spread across the area?, What is it doing?
Effort
Boat transects of 2 to 10 km at 8 to 10 km per h with 2 observers, or snorkel transects of 30 to 60 minutes, 3 to 5 repeats per season, hand capture under permit
Season
Apr, May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Funded project
Licence
All sea turtles are listed. US: a NMFS section 10 permit for any capture or close approach, state permits, and a boat captain licence. UK overseas territories and the Mediterranean need CITES aware national permits; observation from a boat without approach needs no permit but a research permit is expected in marine parks.
How to run it
  1. Plan transects in water under 15 m deep over seagrass or reef, and survey only in Beaufort 2 or less with the sun high for visibility.
  2. Run the boat at 8 to 10 km per h with one observer each side scanning 90 degrees and record each turtle with distance, angle, species, size class and behaviour at first sighting.
  3. For snorkel transects, swim a fixed line at a steady pace with a GPS in a dry bag on a float and count turtles within 5 m each side.
  4. Hand capture, where licensed, is done by a trained swimmer approaching from behind and above, gripping the front of the carapace and bringing the turtle up in under 2 minutes.
  5. On the boat, measure curved carapace length, check for existing tags, fit inconel flipper tags and a PIT tag, and release within 20 minutes at the capture point.
Kit list

The day one mistake: Chasing a turtle that has seen the boat. It dives, surfaces 200 m away and is counted again, and the capture attempt exhausts the animal. Record at first sighting and abandon any capture that goes past 2 minutes.

Reptiles

Basking turtle counts

Count freshwater turtles hauled out on logs and banks from a distance with binoculars or a scope to index abundance and detect invasive sliders. A cheap alternative to trapping for painted, map and pond turtles.

Answers
How many?, Is it here?, How is it spread across the area?
Effort
Fixed vantage points or a 1 to 5 km shoreline route, 10 to 20 minutes per point, late morning on sunny days between 18 and 28 C, 3 to 5 repeats
Season
Apr, May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
None for observation in the UK or US. Landowner permission for reservoir and nature reserve access, and report red eared sliders to the GB Non Native Species Secretariat in the UK.
How to run it
  1. Map the basking logs and banks on the first visit and choose vantage points at least 30 m away so turtles do not drop off as you arrive.
  2. Survey between 10 am and 2 pm on sunny days with light wind and air temperature of 18 to 28 C, when basking peaks.
  3. Approach each point quietly, wait 2 minutes, then scan for 10 to 20 minutes and count every turtle by species and size class using a scope for identification.
  4. Photograph groups so the counts can be checked and to record invasive species such as red eared sliders for the local recorder.
  5. Repeat 3 to 5 times through the season and use the maximum count per point as the index, with survey time and shoreline length as effort.
Kit list

The day one mistake: Walking straight to the water edge. Turtles slide off at 50 m and the count is of ripples. Stop at the vantage point behind cover and let them settle before you count.

Reptiles

Lizard noosing, hand capture and mark recapture

Catch lizards with a noose of fishing line on a pole or by hand, mark them with paint or toe codes, and recapture to estimate population size. The standard for wall lizards, sand lizards, anoles and skinks.

Answers
How many?, Is it here?, What is it doing?
Effort
Plots of 0.5 to 2 ha or 200 to 500 m transects, 2 to 4 h per session in the morning basking window, 3 to 5 capture sessions 3 to 7 days apart
Season
Apr, May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: sand lizards need a Natural England or NatureScot licence to capture; common lizards may be handled but not sold or killed. US: state scientific collecting permits, and a USFWS permit for listed species such as the blunt nosed leopard lizard. Toe clipping is a regulated procedure needing ASPA or IACUC approval.
How to run it
  1. Tie a slip noose of 4 to 6 kg fishing line to the tip of a 1.5 to 3 m telescopic pole and practise on a pen cap until you can drop it over in one motion.
  2. Survey in the first 2 to 3 h of basking on sunny mornings, approaching slowly from the front and lowering the noose over the head, then lifting gently so the lizard hangs by the neck for a second only.
  3. Hold the lizard by the body, never the tail, record SVL, mass, sex and any tail loss, and mark it with a small dot of non toxic paint or a numbered scheme.
  4. Release at the exact capture point within 5 minutes and note the GPS so recaptures give movement as well as numbers.
  5. Run 3 to 5 sessions a few days apart and analyse with a closed population model, recording captures per person hour as the effort.
Kit list

The day one mistake: Grabbing the tail when the noose slips. The tail autotomises, the lizard loses fat stores and its survival for the year, and your recapture data is of a damaged animal. Hand goes over the shoulders or nowhere.

Reptiles

Tortoise burrow surveys with burrow camera

Find gopher or desert tortoise burrows on transects, measure them, and push a burrow camera in to see whether a tortoise is home. The USFWS gopher tortoise line transect distance sampling protocol is the standard for population estimates.

Answers
How many?, Where does it occur across sites?, How is it spread across the area?
Effort
Line transects 10 to 20 m apart across the whole site or distance sampling lines, every burrow scoped with a camera, 1 visit per season, 200 to 400 m of line per hour
Season
Apr, May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
US: gopher tortoise burrow scoping needs a state permit (Florida FWC authorised agent, Georgia DNR) and desert tortoise work needs a USFWS section 10 permit. No UK species; Mediterranean tortoises are protected and any handling needs a national permit.
How to run it
  1. Walk parallel transects 10 to 20 m apart, or distance sampling lines, and record every burrow with GPS and its perpendicular distance from the line.
  2. Measure burrow width at 50 cm inside the entrance with calipers, because width predicts the carapace length of the occupant.
  3. Classify each burrow as active, inactive or abandoned from tracks, plastron slides and debris, then scope it with a burrow camera to confirm occupancy.
  4. Feed the camera slowly along the floor of the burrow to the end or until the tortoise is seen, and never dig, smoke or flood a burrow.
  5. Use only camera confirmed occupancy for the estimate and enter the perpendicular distances in Distance software with at least 60 to 80 occupied burrows.
Kit list

The day one mistake: Pushing the camera in fast and blind. It snags, the tortoise retreats, and the burrow collapses at the bend. Two people, one feeding cable and one watching the screen, and stop at the first sign of resistance.

Fish

Baited remote underwater video, BRUV

Drop a weighted frame with a camera and a bait bag to the seabed for an hour and count the fish that visit. MaxN, the maximum number of each species in one frame, gives a relative abundance that does not double count.

Answers
How many?, Is it here?, How is it spread across the area?
Effort
Camera rigs at 5 to 10 sites 250 to 500 m apart, 60 minute soak each, 1 kg of crushed oily fish in the bait bag, 4 to 6 deployments per site per season, scored as MaxN
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
UK: an MMO marine licence is not needed for a temporary deployment but a Marine Protected Area may need a permit from the IFCA or MMO. US: NOAA or state permits in marine sanctuaries and no take reserves. Bait must be a species legal to possess.
How to run it
  1. Build or buy a frame with a GoPro in a dive housing set to 1080p wide, a 1 to 1.5 m bait arm and 1 kg of crushed pilchard or mackerel in a mesh bag.
  2. Deploy from a boat at 250 to 500 m spacing so bait plumes do not overlap, with a rope and surface float, and log GPS, depth, time and visibility.
  3. Leave each rig for a 60 minute soak; national schemes use 60 minutes and less than that cannot be compared.
  4. Retrieve, download and label footage by site and date, and score every species with MaxN and time of first arrival in a spreadsheet or the GlobalArchive platform.
  5. Record visibility from the footage and the seabed type, because both change MaxN more than fish abundance does.
Kit list

The day one mistake: Scoring total fish counted rather than MaxN. The same wrasse swims through 30 times and the site becomes a hotspot. Count only the most seen at once in one frame per species.

Fish

PIT tagging and antenna detection

Inject a glass passive integrated transponder into an animal and detect it when it passes an antenna in the stream or at a pond entrance. Works for fish, amphibians, reptiles and small mammals and gives survival, movement and growth with no recapture.

Answers
What is it doing?, How many?, Is it here?
Effort
Tag 100 to 500 animals per site, 12 mm tags for fish over 60 mm, antenna array of 1 to 4 loops spanning the channel, checked weekly, running 12 months
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Funded project
Licence
Tagging is a regulated procedure. UK: a Home Office project and personal licence under ASPA plus Environment Agency section 27A consent for fish and a Natural England licence for protected species. US: IACUC approval, a state scientific collecting permit and a USFWS or NMFS permit for listed species.
How to run it
  1. Anaesthetise fish in buffered MS 222 or clove oil under licence, or hold amphibians and reptiles with a wet gloved hand, and inject the tag with a sterile needle into the body cavity or under the skin.
  2. Scan each animal with a handheld reader before release to record the tag number, and hold fish in a recovery bucket until upright and swimming.
  3. Build antenna loops across the channel from 12 gauge wire in flexible conduit, tune them to the reader, and test read range with a tag on a stick at every point in the channel.
  4. Run the reader on a deep cycle battery with a solar panel, download detections weekly, and log any period the antenna was down.
  5. Analyse detections with the tagging records so every fish has a tag date, size and release point, and report tag loss from any recaptures.
Kit list
  • PIT tags and injector (Biomark or Oregon RFID)
  • Handheld PIT reader (Biomark or Oregon RFID)
  • Antenna reader board (Biomark IS1001 or Oregon RFID)
  • Deep cycle battery
  • Solar panel 100 W

The day one mistake: Tagging fish under the minimum size. A 12 mm tag in a 50 mm fish kills it or is shed within a week, and the survival estimate is really a tag loss estimate. Follow the tag to body mass rule and use 8 mm tags for small animals.

Fish

Acoustic telemetry for fish

Surgically implant an acoustic transmitter and moor receivers that log every fish passing within a few hundred metres. The method behind salmon migration, shark movement and barrier passage studies, and the Ocean Tracking Network shares detections across projects.

Answers
What is it doing?, How is it spread across the area?, Is it here?
Effort
10 to 50 tagged fish, receivers 200 m to 1 km apart in a curtain or grid, range tested at deployment, downloaded every 3 to 6 months, running 1 to 3 years
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Funded project
Licence
Surgery is a regulated procedure. UK: a Home Office project and personal licence under ASPA and Environment Agency consent to take and tag fish; a Marine Management Organisation licence may be needed for moorings. US: IACUC approval and state permits, plus NMFS or USFWS permits for listed species; receiver moorings in navigable waters may need a US Coast Guard notice.
How to run it
  1. Choose transmitter size under 2 percent of body mass and a ping interval of 60 to 180 s so battery life covers the study.
  2. Anaesthetise the fish, make a 1 to 2 cm incision in the belly wall, insert the transmitter, close with 2 sutures and return the fish to recovery water; the whole procedure should take under 3 minutes.
  3. Moor receivers on a weighted line 1 to 2 m off the bottom with an acoustic release or a surface float, and range test with a test tag at 100, 200, 400 and 800 m on deployment day.
  4. Download receivers every 3 to 6 months, replace batteries, and record the exact times of any gaps.
  5. Filter false detections (single hits within a 24 h window), then build movement histories per fish and register the array with the OTN or the European Tracking Network.
Kit list
  • Acoustic transmitters (Innovasea (Vemco) or Lotek)
  • Acoustic receivers (Innovasea (Vemco) or Lotek)
  • Surgical kit (veterinary supplier; implant surgery is done or supervised by a licensed vet)
  • Fish anaesthetic MS 222 (a veterinary supplier, on prescription)
  • Deep cycle battery

The day one mistake: Skipping the range test. The receivers are 800 m apart in a noisy estuary where the real range is 200 m, and the fish pass through the gaps undetected. Test on the day and again in a storm and lay the array for the worst case.

Fish

Smolt traps, rotary screw traps and fish counters

A rotating cone in the current catches migrating salmon and trout smolts in a live box so they can be counted, measured and marked before release, while resistivity or video counters count returning adults at a weir. Standard in Environment Agency, Marine Scotland and USFWS salmon monitoring.

Answers
How many?, What is it doing?
Effort
1 rotary screw trap per river in the main flow, checked twice a day at dawn and dusk, 6 to 10 weeks of the spring migration, with a mark recapture release of 50 to 100 fish per week to measure efficiency
Season
Mar, Apr, May, Jun (northern hemisphere; shift 6 months in the south)
Budget
Funded project
Licence
UK: Environment Agency or Marine Scotland section 27A consent and a fish trapping licence, plus a Home Office ASPA licence for marking. US: state permits and an NMFS section 10 permit where the run is listed under the ESA, and IACUC approval for marking.
How to run it
  1. Site the trap in the main current at a depth that keeps the cone turning, anchored to both banks or a bridge, and fit a debris boom upstream.
  2. Check the live box at dawn and dusk without fail, netting fish into a bucket of river water, counting each species and measuring 30 fish per day.
  3. Mark a batch of 50 to 100 smolts each week with a fin clip or dye and release them 500 m upstream; the proportion recaptured is the trap efficiency for that week.
  4. Record trap hours, cone revolutions and river level every check, and stop the cone during spates so fish are not injured in the box.
  5. Divide the daily catch by efficiency to estimate the total run, and reconcile with the adult counter data for the same cohort.
Kit list
  • Rotary screw trap (EG Solutions (US) or a bespoke fabricator; hire through a fisheries consultancy in the UK)
  • Fish measuring board (Forestry Suppliers (US) or NHBS (UK))
  • Bait bucket aerator
  • Fine mesh landing net (a fishing supplier)
  • Water level logger

The day one mistake: Leaving the live box overnight in a rising river. Debris fills the box, oxygen falls, and 200 smolts are dead by morning. Check at dawn and dusk and lift the cone the moment the level rises.

Fish

Larval fish light traps and ichthyoplankton tows

Light traps attract fish larvae and juveniles at night while plankton nets towed behind a boat sample eggs and larvae in the water column. Together they show where and when fish spawn and how many larvae survive.

Answers
How many?, Is it here?, How is it spread across the area?
Effort
Light traps set 1 h after dusk for 8 to 12 h, 3 to 6 traps 100 m apart on new moon nights, or bongo net tows of 5 to 10 minutes at 1 to 2 knots, 3 to 5 stations, fortnightly through the spawning season
Season
Apr, May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
UK: Environment Agency section 27A consent to take fish including larvae, and MMO or IFCA permits in marine protected areas. US: state scientific collecting permits and NMFS permits in federal waters or for listed species. Formalin needs COSHH or OSHA handling controls.
How to run it
  1. Assemble a light trap from a clear box with funnel entries and a waterproof LED, or buy a quatrefoil trap, and moor it 1 m below the surface on a weighted line.
  2. Set traps 1 h after sunset on dark nights and retrieve at dawn, rinsing the catch into a labelled jar of 70 percent ethanol or 5 percent buffered formalin.
  3. For tows, fit a bongo or 500 micron plankton net with a flowmeter in the mouth, tow at 1 to 2 knots for 5 to 10 minutes, and record the flowmeter reading to convert to volume.
  4. Rinse the cod end into a jar, label inside and out with site, date and gear, and keep it out of the sun.
  5. Sort under a microscope, identify larvae with a regional key, and report numbers per trap night or per 100 m3 of water.
Kit list

The day one mistake: Setting the traps on a full moon. Moonlight swamps the trap light and the catch drops by 90 percent, so the season looks empty. Set on the new moon and the nights either side, and record moon phase every time.

Fish

Lamprey ammocoete surveys

Electrofish or scoop the fine sediment margins of a river to catch lamprey larvae, which live buried for 3 to 7 years and are the only reliable stage to survey. The Environment Agency and JNCC ammocoete method sets the quadrat and effort rules.

Answers
Is it here?, How many?, How is it spread across the area?
Effort
5 to 10 quadrats of 0.5 m2 in silt beds per 100 m reach, 3 minutes of electrofishing per quadrat, or bucket sampling of 5 to 10 scoops, water below 18 C, 1 visit per summer
Season
Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
UK: Environment Agency section 27A consent for electrofishing, plus SSSI or SAC assent from Natural England or NatureScot where the river is designated; lamprey are Annex II species so no species handling licence applies. US: state scientific collecting permit and, for Pacific lamprey in some states, tribal consultation.
How to run it
  1. Walk the reach and mark every patch of soft silt or sand with organic matter in slack water, which is the only habitat ammocoetes use.
  2. Place a 0.5 m2 quadrat on the silt and electrofish it in slow pulses with a lamprey setting (low frequency, 3 to 5 Hz) for 3 minutes while a second person nets larvae as they emerge.
  3. Where electrofishing is not licensed, take 5 to 10 scoops of sediment with a bucket into a sieve and wash gently in a tray.
  4. Measure every larva to the millimetre and separate brook and river lamprey from sea lamprey by pigment pattern on the tail and oral hood.
  5. Return larvae to the same silt bed and report density per m2 by habitat type, with sediment depth and water temperature.
Kit list
  • Backpack electrofisher (Smith Root or Electracatch; hire in the UK from an Environment Agency approved contractor)
  • Quadrat frame 50 cm (NHBS (UK), Forestry Suppliers (US), or make from PVC pipe)
  • Fine mesh landing net (a fishing supplier)
  • Sieve 1 mm
  • Waders

The day one mistake: Fishing the gravel riffle where you expect fish. Ammocoetes live only in soft silt, so a riffle survey scores zero and the river is wrongly reported as empty. Look for silt with decaying leaves in the slack edges.

Fish

Trawl and beam trawl surveys

A net towed along the seabed by a research vessel catches bottom fish and shellfish at fixed stations so that stock size and age structure can be tracked year on year. The ICES International Bottom Trawl Survey and the NOAA groundfish surveys are the reference designs.

Answers
How many?, How is it spread across the area?, Is it here?
Effort
Tows of 15 to 30 minutes at 2 to 4 knots on a fixed station grid, 1 tow per station, 20 to 100 stations per survey, repeated annually in the same season
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Funded project
Licence
UK: an MMO scientific fishing dispensation and a Cefas or Marine Scotland survey licence, and the skipper needs a commercial fishing certificate. US: NMFS scientific research permit and state permits within 3 nautical miles. Both need a vessel safety certificate and crew safety training.
How to run it
  1. Use the standard gear for the region (GOV trawl for the North Sea IBTS, 2 m or 4 m beam trawl for flatfish and shrimp) and never change mesh or rigging between years.
  2. Tow for exactly 15 minutes (beam) or 30 minutes (otter trawl) at the standard speed with a start and end position, and record warp length and wing spread.
  3. Sort the whole catch by species on deck, weigh each species, and measure the length of all fish or a random subsample of 100 per species.
  4. Take otoliths and scales from a stratified length sample for ageing, and record maturity stage and sex.
  5. Log depth, bottom temperature and salinity with a CTD at each station and report catch per hour or per swept area.
Kit list

The day one mistake: Changing the tow speed because the catch is poor. Swept area is the denominator, so a faster tow makes the fish look denser than last year. The standard speed and duration are the survey, not a guideline.

Fish

Hydroacoustic echo sounder surveys

A scientific echo sounder sends pulses down through the water and the returned echoes give the number and size of fish in the column across the whole lake or shelf in one night. The standard for pelagic species such as vendace, coregonids, sprat and herring, and set by ICES and the EU Water Framework Directive guidance.

Answers
How many?, How is it spread across the area?
Effort
Zigzag or parallel transects at 5 to 10 knots covering the whole lake or a fixed sea area, run at night, calibrated with a tungsten carbide sphere before each survey, 1 to 2 surveys per year
Season
Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Funded project
Licence
None for the sounder itself in the UK or US. Ground truth netting needs Environment Agency section 27A consent in the UK and state or NMFS permits in the US, and night boat work on lakes needs the owner's permission and a boat safety plan.
How to run it
  1. Calibrate the transducer with a tungsten carbide sphere at the start of every survey and record the calibration gain.
  2. Run transects at night when fish leave the bottom and disperse, at 5 to 10 knots, with the transducer pole mounted 0.5 to 1 m below the surface.
  3. Plan parallel or zigzag lines at a spacing that gives a coverage degree of at least 6 (total line length divided by the square root of the area).
  4. Ground truth the echoes with a trawl, gill net or camera on the same night so echo sizes can be turned into species and lengths.
  5. Process in Echoview or Sonar5, remove surface and bottom dead zones, and report fish density per hectare or biomass per square kilometre with the coverage degree.
Kit list
  • Scientific echo sounder (Kongsberg Simrad EK80 or Biosonics DT X)
  • Calibration sphere (Kongsberg Simrad or Biosonics)
  • Transducer pole mount (make from aluminium tube or a boat builder)
  • GPS with NMEA output
  • Deep cycle battery

The day one mistake: Surveying in the afternoon when the fish are in a tight layer on the bottom inside the dead zone. The lake looks empty and the biomass is a fraction of the truth. Run at night, and repeat the same night pattern every year.

Freshwater invertebrates and plankton

Crayfish hand search, night torching and refuge traps

Find crayfish by lifting stones by hand, by torching the shallows at night when they are active, and by leaving artificial refuge traps overnight. Used for the white clawed crayfish protocol of the Environment Agency and for signal crayfish spread mapping.

Answers
Is it here?, How many?, How is it spread across the area?
Effort
Hand search up to 100 refuges per site or 10 person minutes per 10 m of bank, torching after dark for 30 minutes per 100 m, 10 refuge traps per site left 24 h, 3 visits July to October
Season
Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: white clawed crayfish are protected and hand searching or trapping needs a Natural England, NRW or NatureScot licence, plus EA consent to trap and a trapping authorisation for signal crayfish. US: a state scientific collection permit; several states ban live transport of any crayfish.
How to run it
  1. Check the biosecurity plan first: survey any native site before any invasive site on the same day, and disinfect and dry all kit between waters.
  2. In daylight work upstream lifting every stone larger than your hand for a fixed time, placing each stone back as it was and holding the net downstream of your hands.
  3. After dark walk the same reach with a strong torch and count every crayfish seen on the bed for a timed 30 minutes.
  4. Set 10 refuge traps of bundled plastic tubes on the bed, each fitted with an otter guard, anchored and marked, and lift them after 24 h into a white tray.
  5. Identify each animal to species, record sex and carapace length, and return natives at the capture point; invasive species must not be returned to the water and must be killed humanely on site by freezing or destruction of the head, as your licence requires.
Kit list

The day one mistake: Putting a captured signal crayfish back because it was not the target. Returning an invasive species is illegal in the UK and spreads plague. Read the rule for your area before the first trap goes in and take a bucket and a lid.

Freshwater invertebrates and plankton

Dragonfly exuviae searches and larval sampling

Collect the cast larval skins left on emergent stems and sample larvae with a pond net to prove breeding rather than just visiting adults. Exuviae are the British Dragonfly Society standard for confirming a breeding site.

Answers
Is it here?, How many?, What is it doing?
Effort
Search 20 m of margin in 20 minutes for exuviae every 7 to 14 days through the flight season, plus 10 timed 1 minute net sweeps per pond in spring
Season
May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: no licence for exuviae; larvae of Norfolk hawker and a few other species are protected and need a licence to catch. US: no federal permit; state collecting permits apply in some states, and refuges need a special use permit.
How to run it
  1. Mark a 20 m stretch of emergent vegetation and search it for a fixed 20 minutes on each visit, checking stems, trunks and bridge piers to 2 m above water.
  2. Lift each exuvia by the stem with tweezers, drop it into a labelled pot and note the height above water and the plant it was on.
  3. In April to June sweep a pond net for 1 minute through 10 stations of submerged and emergent vegetation and tip into a white tray.
  4. Identify larvae in the tray with a hand lens, record instar as final or earlier, return them within 10 minutes, and Check Clean Dry the net and tray before the next pond.
  5. Identify exuviae at home under a microscope with a key, and keep them in dry pots as vouchers for county recorders.
Kit list

The day one mistake: Searching exuviae only on sunny afternoons. Most emergence happens overnight and skins fall in wind within a day or two, so the first search after a dry still night is the one that counts. Go early and go often.

Freshwater invertebrates and plankton

Surber and Hess quantitative sampling

Sample a fixed area of stream bed with a Surber (0.09 sq m) or Hess (0.086 sq m) frame so invertebrate counts convert to density per square metre. Used where the kick sample is not enough and you need numbers, following EPA rapid bioassessment and EA guidance.

Answers
How many?, How is it spread across the area?, Is it here?
Effort
5 Surber samples per riffle of 0.09 sq m each, 60 seconds of disturbance per sample, 1 riffle per site, spring and autumn
Season
Mar, Apr, May, Sep, Oct, Nov (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
UK: no licence for invertebrates, but EA or NRW consent for work in the channel of a main river and landowner permission. US: state scientific collection permit in most states; Army Corps or state permits are not needed for hand sampling.
How to run it
  1. Work upstream so you never sample bed you have walked over, choosing 5 random points across one riffle at 10 to 30 cm depth.
  2. Set the frame on the bed with the net mouth facing downstream and press the skirt down to seal it.
  3. Scrub every stone inside the frame into the net, then stir the gravel to 5 cm for 60 seconds by hand.
  4. Wash the net contents into a jar, label inside and out, preserve in 70 percent ethanol, and Check Clean Dry waders, net and frame before the next site.
  5. Sort the whole sample in the lab, identify to family or species, and divide counts by the frame area for density per square metre.
Kit list

The day one mistake: Sampling a nice looking riffle and calling it the site. Five samples from one chosen spot cannot represent a reach. Randomise the points across the whole riffle with a table or a thrown quadrat.

Freshwater invertebrates and plankton

eDNA metabarcoding for invertebrate communities

Filter water and sequence the trace DNA to list the invertebrate taxa present in a river or pond without sorting a sample. Best used alongside a kick sample as the reference lists are still incomplete for many groups.

Answers
Is it here?, How is it spread across the area?, Where does it occur across sites?
Effort
3 replicate 1 to 2 litre samples per site filtered on 0.45 micron filters, 1 field blank per site, 1 visit per season
Season
Apr, May, Jun, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Funded project
Licence
UK: no licence for water sampling, but landowner and site consent; Natural England does not yet accept invertebrate eDNA as a stand alone survey. US: state collection permits are usually not needed for water; refuge or park research permits still apply.
How to run it
  1. Book a lab that runs COI metabarcoding first and get their sampling kit, as the filters and preservative must match their pipeline.
  2. Wear fresh nitrile gloves at every site, Check Clean Dry boots and waders before entering, and open a bottle of distilled water at the site to make a field blank.
  3. Collect 3 replicates of 1 to 2 litres at points 10 m apart, upstream of where you stand, and filter each on site with the syringe kit until it clogs.
  4. Record the volume filtered, add the preservative buffer, seal each filter in its tube and keep it cold and dark.
  5. Send samples within 24 h or freeze them, and when results come back keep the raw read counts and the negative control results with the species list.
Kit list

The day one mistake: Sampling the downstream site first and walking upstream. DNA from boots and kit carries between sites and the upstream sample then shows species that live 2 km away. Always work from the cleanest or most upstream site down.

Freshwater invertebrates and plankton

Invasive non native species checks with Check Clean Dry

Look for the main freshwater invaders (killer shrimp, zebra mussel, signal crayfish, floating pennywort, New Zealand pygmyweed) at every site and follow the Check Clean Dry biosecurity routine so you never move them. A habit that sits beneath every other freshwater method.

Answers
Is it here?, How is it spread across the area?
Effort
20 minute search of margin, bed and kit at every site, checked on arrival and on leaving, every visit
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: it is an offence to release or allow to escape any Schedule 9 species, and a licence is needed to keep or transport several. US: Lacey Act and state laws forbid transport of listed aquatic invasives; some states require boat and gear inspection certificates.
How to run it
  1. Before the season print the GB Non Native Species Secretariat or your state ID sheets for the 10 target species and keep them in the kit bag.
  2. On arrival spend 10 minutes checking the margin and any hard surfaces for shells, weed rafts and shrimp in a white tray.
  3. On leaving check every net, wader and rope for mud, weed and animals, and pick them off into a bag for disposal in general waste.
  4. Clean kit with water at the site or a 1 percent Virkon Aquatic dip (10 g per litre) for 15 minutes, then dry it completely for 48 h before the next water, and keep a second set of kit for known infested sites.
  5. Report every new sighting with a photograph to the national alert scheme within 24 h, and never return a suspected invader to the water.
Kit list

The day one mistake: Rinsing waders in the next river to clean them. That is how killer shrimp moved between reservoirs. Clean at the car with your own water and let the kit dry fully, or use a dedicated set for each catchment.

Terrestrial invertebrates and pollinators

Beating trays for canopy invertebrates

Hold a white tray under a branch and strike the branch so the insects fall onto the cloth, then collect them with a pooter. The standard way to sample beetles, bugs and caterpillars from foliage.

Answers
Is it here?, How many?, How is it spread across the area?
Effort
10 trees per site, 5 sharp taps on each of 4 branches per tree, 1 visit per month May to September
Season
May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: none for common species; landowner permission and a Natural England licence if you might take a listed species. US: none on private land; National Forests and refuges require a collecting permit.
How to run it
  1. Choose 10 trees of the target species along a path and tag them so the same trees are sampled each month.
  2. Hold the tray 30 cm under a branch at head height and give 5 sharp taps with a stick within 3 seconds, then stop.
  3. Suck small insects into the pooter and drop larger ones into a pot with a paintbrush before they fly, working fast in the first 30 seconds.
  4. Record the tree, branch height and species then repeat on 3 more branches on different sides of the tree.
  5. Identify in the field where you can and preserve the rest in 70 percent alcohol with a pencil label inside the tube.
Kit list
  • Beating tray (Watkins and Doncaster (UK) or Forestry Suppliers (US), or make from a white sheet on two crossed canes)
  • Pooter (Watkins and Doncaster (UK) or Forestry Suppliers (US))
  • Specimen tubes
  • Fine paintbrush
  • Hand lens 10x

The day one mistake: Beating wet foliage after rain. Everything sticks to the leaves and the count is a fraction of the true number. Wait until the canopy is dry and beat in still weather.

Terrestrial invertebrates and pollinators

Butterfly egg, larval and web searches

Search the larval foodplant for eggs, caterpillars or silk webs, which proves breeding and can be counted when the adults are hard to see. Butterfly Conservation uses this for brown hairstreak eggs, marsh fritillary webs and pearl bordered fritillary larvae.

Answers
Is it here?, How many?, What is it doing?
Effort
Timed 30 minute searches of foodplants, or 100 plants checked per plot, 2 to 3 visits per species at the right stage
Season
Nov, Dec, Jan, Feb, Mar, Apr, May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: several species are protected from taking or disturbance and need a Natural England licence to handle; searching without handling needs only landowner permission. US: no federal permit unless the species is listed, then a USFWS permit; state parks need research permits.
How to run it
  1. Read the species guidance for the stage and month; brown hairstreak eggs are counted on blackthorn in winter, marsh fritillary webs in late August and September.
  2. Mark a search plot or a fixed length of hedge and search for a timed 30 minutes or check 100 plants, whichever the protocol says.
  3. Check the underside of leaves and the stem forks at the height the guidance names and count each egg, larva or web once.
  4. Note the plant species, its height, sun or shade, and mark larger finds with a discreet tag so they can be followed.
  5. Never remove eggs or larvae; photograph and log the location for the county recorder.
Kit list

The day one mistake: Counting brown hairstreak eggs on old thick blackthorn. Females lay on 1 to 3 year old growth at the fork of a twig, so time spent on old wood is wasted. Search young sunny growth at waist height.

Terrestrial invertebrates and pollinators

Orthoptera surveys with bat detector and sweep net

Walk a transect with a bat detector to hear the songs of grasshoppers and bush crickets that are too high for most ears, and sweep the vegetation to confirm identity. The Orthoptera Recording Scheme method.

Answers
Is it here?, How many?, How is it spread across the area?
Effort
500 m transect walked in 30 minutes with the detector on 25 to 40 kHz, plus 20 sweeps of the net at 5 stops, 2 visits in warm afternoons
Season
Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: none for observation; wart biter and field cricket are protected and need a licence to catch. US: none on private land; state permits for listed species and protected areas.
How to run it
  1. Survey on a dry afternoon over 18 C, between 13:00 and 17:00 for grasshoppers and into dusk for bush crickets.
  2. Walk the transect slowly with the detector set to 25 kHz for grasshoppers and 40 kHz for bush crickets, stopping when you hear a song.
  3. Count singing males within 5 m of the line and identify the song with a reference recording.
  4. At 5 stops make 20 sweeps of the net through the sward and tip the catch into a pot to confirm species and sex.
  5. Record nymphs separately and release everything at the stop.
Kit list

The day one mistake: Surveying in the morning. Most grasshoppers do not sing until the ground has warmed after midday, so a morning transect records a tenth of what is there. Come back after lunch in sun.

Terrestrial invertebrates and pollinators

Saproxylic beetle surveys (flight interception and vane traps on veteran trees)

Hang flight interception or window traps on trees with deadwood features to catch the beetles that breed in decaying wood. The standard for scoring veteran tree sites by the Saproxylic Quality Index.

Answers
Is it here?, How is it spread across the area?, How many?
Effort
1 trap per veteran tree on 10 to 20 trees, hung at 2 to 4 m, emptied every 2 weeks May to August
Season
May, Jun, Jul, Aug (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
UK: none for common beetles; landowner consent and a Natural England licence if a protected species such as violet click beetle could be caught. US: a collecting permit on federal and state land; nothing on private land.
How to run it
  1. Pick 10 to 20 trees with hollows, rot holes, sap runs or dead limbs and photograph each feature with a tree tag number.
  2. Hang the trap 2 to 4 m up next to the deadwood feature with a rope over a branch so it can be lowered.
  3. Fill the collecting bottle with 50 percent propylene glycol and a drop of detergent and empty it every 14 days.
  4. Strain the catch into 70 percent ethanol, label with tree ID and dates, and count the trap days.
  5. Sort to family in the lab, send difficult beetles to a specialist, and calculate the index from the published species list.
Kit list

The day one mistake: Hanging traps in the open on a pole. Saproxylic beetles fly to the tree feature, not the field, so a trap 5 m from the hollow catches flies. Hang it touching the trunk beside the feature.

Terrestrial invertebrates and pollinators

Stag beetle dusk transects

Walk a fixed route at dusk and count flying and walking stag beetles, following the PTES Great Stag Hunt method. One of the few beetle surveys the public can run.

Answers
Is it here?, How many?, How is it spread across the area?
Effort
500 m transect walked slowly in 30 minutes, starting 15 minutes before sunset, once a week for 6 weeks from late May to mid July
Season
May, Jun, Jul (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: none; stag beetle is protected only from sale, not from observation. US: no federal permit for observation of any stag beetle.
How to run it
  1. Choose a route through gardens, parks or wood edge with old stumps and walk it on warm still evenings above 15 C.
  2. Start 15 minutes before sunset and walk the 500 m at a slow steady pace for 30 minutes, once a week for 6 weeks.
  3. Count every beetle, note sex, whether flying or walking, and any found dead on the pavement.
  4. Log the exact spot on GPS for each sighting and the nearest stump or deadwood.
  5. Report every record to the PTES or your local recording scheme and repeat the route on the same weekday each year.
Kit list

The day one mistake: Walking at sunset then giving up. Peak flight is 30 to 60 minutes after sunset once the air is still, so a survey that ends at sunset misses the main pulse. Stay until it is fully dark.

Terrestrial invertebrates and pollinators

Ant surveys (baiting cards, nest counts)

Lay baits of tuna and honey on cards to draw foraging ants, and count nest mounds in a plot to estimate colony density. Bait cards follow the standard ant sampling methods of Agosti et al. and the plot count follows the UK wood ant survey guidance.

Answers
Is it here?, How many?, How is it spread across the area?
Effort
20 bait cards per site at 10 m spacing checked after 60 minutes, plus a 100 sq m plot for nest counts, 2 visits
Season
May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: none; wood ants are not protected but many sites are SSSIs so ask the site manager. US: none on private land; federal land needs a collecting permit and several states protect harvester ants from collection.
How to run it
  1. Set out 20 index cards at 10 m spacing along a line, each with a teaspoon of tuna and a drop of honey, between 10:00 and 16:00 on a warm dry day.
  2. After 60 minutes photograph each card, count the ants by species and collect 5 workers per species into alcohol.
  3. Mark out a 10 by 10 m plot and search it for nests by turning stones and looking for mounds and soil craters.
  4. Measure each wood ant mound height and diameter and mark it with GPS so it can be re found.
  5. Identify with a key under a microscope and send difficult specimens to a recorder.
Kit list

The day one mistake: Checking bait cards after 5 hours. Ants recruit in tens of minutes and the dominant species then excludes the rest, so a late check shows one species per card. Check at 60 minutes exactly.

Terrestrial invertebrates and pollinators

Spider and grassland suction sampling (G vac)

Use a converted garden vacuum to suck spiders, bugs and beetles out of short vegetation over a known area. Gives density per square metre and catches the small ground layer species a sweep net misses.

Answers
How many?, Is it here?, How is it spread across the area?
Effort
10 samples per site, each 10 placements of 10 seconds on a 0.1 sq m nozzle, 2 visits in dry weather
Season
May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
UK: none; landowner permission and a licence if ladybird spider or fen raft spider could be taken. US: none on private land; federal land needs a permit.
How to run it
  1. Fit a fine mesh bag inside the nozzle of a leaf blower vacuum and mark the nozzle area, usually 0.1 sq m.
  2. Sample only when the sward is dry, placing the nozzle firmly onto the ground for 10 seconds at 10 spots 1 m apart.
  3. Tip the bag into a large tray, pick the catch into alcohol with a pooter and note the sward height.
  4. Repeat for 10 samples per site along a random line and record the total area as 1 sq m per sample.
  5. Sort in the lab to family and count, then send spiders to a recorder for species.
Kit list

The day one mistake: Sampling after dew. Wet vegetation clogs the bag and the animals stay glued to the sward, so morning counts are far lower than afternoon counts at the same site. Wait until the grass is dry to the touch.

Terrestrial invertebrates and pollinators

Glow worm transects

Walk a route after dark and count the glowing females, following the UK Glow worm Survey method. Cheap, pleasant and the only way to count a species you cannot see by day.

Answers
Is it here?, How many?, How is it spread across the area?
Effort
Fixed transect of 500 m to 1 km walked between 22:30 and 00:00, 5 to 8 visits June to July at weekly intervals
Season
Jun, Jul (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: none. US: none for observation of fireflies or glow worms; state park night access may need permission.
How to run it
  1. Choose a route along a bank, verge or old grassland and walk it once in daylight to learn the ground.
  2. Start 1 h after sunset on a warm still night and walk slowly without a torch, using red light only to write.
  3. Count every glowing female within 5 m each side and mark the position on GPS, noting the vegetation height.
  4. Count glowing larvae separately, which glow more weakly and move.
  5. Repeat weekly for at least 5 nights to find the peak, and report the peak count as the transect result.
Kit list

The day one mistake: Walking with a white headlamp on. Females stop glowing when lit and the count collapses. Walk dark, let your eyes adjust for 10 minutes, and use red light only to write.

Terrestrial invertebrates and pollinators

Tick drag sampling

Drag a white cloth over vegetation so questing ticks attach, then count them per 100 sq m. The CDC and UK Health Security Agency method for tick density and disease risk mapping.

Answers
How many?, Is it here?, How is it spread across the area?
Effort
10 drags of 10 m each per site with a 1 sq m cloth, checked every 10 m, 1 visit per month April to October between 10:00 and 16:00
Season
Apr, May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: none; landowner permission. US: none on private land; state and national parks need a research permit, and live tick transport across state lines may need a USDA permit.
How to run it
  1. Sew a 1 by 1 m white flannel cloth to a pole with a rope and treat clothing with permethrin the day before.
  2. Drag the cloth at walking pace for 10 m, stop, and check both sides in good light.
  3. Pick ticks off with fine forceps into a tube of ethanol and record stage as larva, nymph or adult.
  4. Repeat for 10 drags along a random route through the habitat and record temperature and humidity.
  5. Check your own clothing fully at the end and report density as ticks per 100 sq m.
Kit list

The day one mistake: Dragging through wet grass early in the morning. Ticks do not quest on wet vegetation and the cloth picks up nothing. Sample when the sward is dry and humidity is between 50 and 85 percent.

Terrestrial invertebrates and pollinators

Mosquito trapping and larval dipping

Run a baited light or BG trap overnight for adult mosquitoes and dip standing water with a ladle for larvae. Used by public health teams and for invasive Aedes surveillance.

Answers
Is it here?, How many?, How is it spread across the area?
Effort
1 CO2 or BG trap per site run 1 night per fortnight, plus 20 larval dips of 350 ml per water body, April to October
Season
Apr, May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
UK: none; landowner permission and consent from the site if it is a reserve. US: none for private land; mosquito control districts often require notification and CO2 cylinders need transport rules followed.
How to run it
  1. Hang the trap at 1.5 m in shade near vegetation with the CO2 or lure fitted and switch it on 1 h before dusk.
  2. Collect the catch bag at dawn, kill in a freezer and count by species and sex under a lens.
  3. At every standing water body take 20 dips with a 350 ml dipper along the edge, tilting slowly to avoid a shadow, and Check Clean Dry the dipper between water bodies.
  4. Count larvae and pupae per dip, pipette a sample into alcohol for ID and note the water type.
  5. Set larval traps of black cups with water for invasive Aedes and check for eggs on the paper strip after 7 days.
Kit list
  • Mosquito trap (BG Sentinel by Biogents or CDC light trap via Clarke or John W. Hock (US); NHBS (UK))
  • Larval dipper (BioQuip style dipper via Forestry Suppliers (US), or make from a 350 ml cup on a 1 m pole)
  • Pipettes
  • Specimen tubes
  • Cool box with ice packs

The day one mistake: Dipping straight into the water and creating a shadow. Larvae dive at the first movement and the dip is empty. Approach from the sun side, hold still for 30 seconds, then dip in one slow movement.

Terrestrial invertebrates and pollinators

Dung beetle baited pitfalls

Sink a pot in the ground with fresh dung suspended above it to catch the beetles that fly to it. The Dung beetle UK Mapping Project and tropical rapid assessment method.

Answers
How many?, Is it here?, How is it spread across the area?
Effort
10 baited pitfalls per site at 50 m spacing, left 48 h, 1 visit per month April to October
Season
Apr, May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: none; landowner permission and confirmation that no wormer has been used. US: none on private land; grazing allotments on federal land need a permit.
How to run it
  1. Ask the farmer for fresh cattle or sheep dung from animals not treated with ivermectin in the last 4 weeks.
  2. Sink a 1 litre pot flush with the ground and add 5 cm of water with a drop of detergent.
  3. Wrap 200 g of dung in muslin and hang it 5 cm above the pot on a wire; place a rain cover on stakes.
  4. Set 10 traps at 50 m spacing and collect after 48 h, straining beetles into alcohol.
  5. Record the livestock present, sward height and soil type, and identify beetles with a key or send to the mapping project.
Kit list

The day one mistake: Using dung from wormed cattle. Ivermectin residues repel or kill the beetles and the trap catches nothing for weeks. Ask about treatment dates before collecting the bait.

Plants and vegetation

Invasive plant mapping

Walk a site and map every stand of Japanese knotweed, Himalayan balsam, giant hogweed or the local invaders with GPS and a size class. The base map for any control programme.

Answers
How is it spread across the area?, Is it here?, How many?
Effort
Walk the whole site or 100 m bank sections, GPS every stand over 1 sq m, 1 visit at peak growth and 1 after control
Season
May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: none for mapping; it is an offence to plant or cause to grow Schedule 9 species, and knotweed soil is controlled waste. US: none for mapping; state noxious weed laws may require reporting.
How to run it
  1. Learn the 5 to 10 target species from the GB Non Native Species Secretariat or state sheets and survey in June to September when they are tall.
  2. Walk the whole site in parallel lines 20 m apart, or every bank of a river in 100 m sections.
  3. At each stand record the GPS point or polygon, the longest dimension, the density class (scattered, patchy, dense) and the growth stage.
  4. Photograph each stand with a scale object and check 20 m downstream and downwind for outliers.
  5. Clean boots and tools of seed and rhizome before leaving, and repeat after control to score change.
Kit list

The day one mistake: Mapping only the big stands. The small outliers 20 m away are what spread the plant, and they are the cheapest to remove. Walk the full site systematically, not just the obvious patches.

Plants and vegetation

Hedgerow surveys (structure and species)

Record the woody species, height, width, gaps and base condition of a hedge in 30 m sections, following the UK Hedgerow Survey Handbook and the PTES Great British Hedgerow Survey.

Answers
How is it spread across the area?, Is it here?, How many?
Effort
30 m sections of hedge, woody species counted in each, 1 visit per hedge in leaf, 5 to 20 hedges per farm
Season
May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: none; landowner permission, and note that hedges over 20 m are protected under the Hedgerows Regulations. US: none; hedgerows have no protection but landowner permission is required.
How to run it
  1. Map every hedge on the site and give each a number, then choose the central 30 m of each or divide long hedges into 30 m sections.
  2. In each section list every woody species and count them, noting the dominant one and any trees over 4 m.
  3. Measure height and width at the middle of the section, and estimate gaps as a percent of length.
  4. Score the base: margin width, bank or ditch, bare soil, nettles and the number of woodland ground flora species.
  5. Record management (trimmed, laid, coppiced, unmanaged) and classify each hedge to the Handbook condition categories.
Kit list

The day one mistake: Counting species along the whole hedge. The Handbook uses 30 m sections so results compare between hedges and years; a 200 m walk with a species list cannot be repeated. Mark section ends with GPS.

Plants and vegetation

Veteran tree and tree health surveys

Record girth, form and the deadwood features of each old tree for the Ancient Tree Inventory, and check for signs of ash dieback, Dutch elm disease, Phytophthora and bleeding cankers. One survey feeds both conservation and safety.

Answers
How is it spread across the area?, Is it here?, How many?
Effort
1 tree per record, 10 to 20 minutes each, all trees over the girth threshold on the site, 1 visit plus annual health check
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: none; Tree Preservation Orders and felling licences do not stop measuring, and landowner permission is needed. US: none; report suspected regulated pests to the state department of agriculture.
How to run it
  1. Walk the site and tag or GPS every tree with a girth over 3 m at 1.5 m, or the species threshold in the Woodland Trust guidance.
  2. Measure girth at 1.5 m with a tape, or below the swelling if the trunk is fluted, and record species and form (maiden, pollard, coppice).
  3. Tick the veteran features present: hollowing, rot holes, dead limbs, sap runs, fungal brackets, epiphytes, bark loss.
  4. Look for disease signs by species: diamond lesions and crown dieback in ash, bleeding cankers on horse chestnut, wilting in elm, and photograph any find.
  5. Submit each tree to the Ancient Tree Inventory or your state champion tree register and repeat the health check each summer.
Kit list

The day one mistake: Measuring girth at whatever height is easy. A girth taken at 1 m on one tree and 1.5 m on the next cannot be compared. Use 1.5 m every time and note the exception when a burr forces you lower.

Plants and vegetation

Phenology monitoring

Record the dates of first leaf, first flower, fruit and leaf fall on the same marked plants each year to track climate response. Nature's Calendar run by the Woodland Trust in the UK and the USA National Phenology Network use this protocol.

Answers
What is it doing?, How is it spread across the area?, Is it here?
Effort
5 to 10 tagged plants per species, checked every 7 days spring and autumn and every 14 days in summer, every year
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: none; landowner permission. US: none; NPN observers on refuges need a special use permit for repeat access.
How to run it
  1. Choose 5 to 10 plants of each species that you can reach every week and tag them with a number.
  2. Visit weekly from February and score each phenophase as present or absent using the network definitions (bud burst, first leaf, first flower, open flowers, ripe fruit, leaf colour, leaf fall).
  3. Estimate the intensity as a percent class of buds open or leaves fallen.
  4. Photograph each plant on every visit from the same spot so dates can be checked.
  5. Enter the data into the network website the same week and keep the same plants for as many years as you can.
Kit list

The day one mistake: Recording only the first flower date. One date per year is a weak signal with no error bar. Score presence and intensity of every phase on every visit so the start, peak and end can all be estimated.

Plants and vegetation

Saltmarsh zonation transects

Run a line from the low marsh up to the terrestrial edge and record species cover and height in quadrats, so the zonation and any squeeze against sea walls can be tracked. The Environment Agency saltmarsh monitoring and NOAA marsh vegetation protocol.

Answers
How is it spread across the area?, How many?, Is it here?
Effort
3 to 5 transects from creek edge to upper marsh, 1 by 1 m quadrats every 10 m or at each zone change, 1 visit at peak growth
Season
Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: most saltmarsh is SSSI so Natural England consent for pegging, and Crown Estate or landowner permission. US: state coastal zone permits for marking posts, and NOAA or refuge permits on federal marsh.
How to run it
  1. Check tide tables and start 2 h before low water on a spring tide, with an escape route agreed.
  2. Set a transect from the pioneer zone to the sea wall or upper limit with a fixed start post and a bearing.
  3. Place a 1 by 1 m quadrat every 10 m or at every visible change of community and record percent cover of every species and bare mud.
  4. Measure the elevation of each quadrat with a level or GPS with RTK where you have it, and note creek and pan edges.
  5. Mark quadrat corners with pegs, photograph from the landward side and repeat annually in the same month.
Kit list

The day one mistake: Starting a transect at the sea wall and walking out as the tide turns. Creeks fill behind you and mud is soft on the pioneer zone. Start at the outer end on a falling tide and work back to land.

Plants and vegetation

Dune vegetation transects

Walk a line from the beach across the dune ridges and slacks recording species cover and the ground profile, to track succession, bare sand and scrub invasion. The Sand Dune Survey of Great Britain method.

Answers
How is it spread across the area?, How many?, Is it here?
Effort
3 to 5 transects from strandline to fixed dune, 2 by 2 m quadrats every 10 m, profile levelled every 5 m, 1 visit per year
Season
May, Jun, Jul, Aug (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: most dune systems are SSSI or SAC so consent for posts from Natural England, NRW or NatureScot. US: state coastal permits and National Seashore research permits for any marker.
How to run it
  1. Set a transect perpendicular to the shore from the strandline to the back of the fixed dune and mark the start with a post and GPS.
  2. Level the ground profile every 5 m with a clinometer or level and staff to draw a cross section.
  3. Place a 2 by 2 m quadrat every 10 m and record percent cover of every species, bare sand, litter and moss.
  4. Classify each quadrat to a dune zone (embryo, mobile, semi fixed, fixed, slack, scrub) and note rabbit grazing and trampling.
  5. Photograph each quadrat and the transect line and repeat in the same month each year.
Kit list
  • Quadrat 2 by 2 m (make from four 2 m canes and string, or NHBS (UK))
  • Tape measure 50 m
  • Clinometer
  • Ranging poles (Forestry Suppliers (US) or NHBS (UK))
  • Wildflower field guide (NHBS (UK) or a bookshop (US))

The day one mistake: Placing quadrats where the vegetation is interesting. That biases every cover value upward and hides bare sand loss, which is the thing the survey exists to measure. Keep the fixed 10 m spacing whatever the ground looks like.

Plants and vegetation

Seed rain traps and seed bank germination

Catch falling seed in funnel traps to measure seed rain, and germinate soil cores in a greenhouse to count the viable seed bank. Together they show whether a site can restore itself.

Answers
How many?, How is it spread across the area?, Is it here?
Effort
10 to 20 seed traps of 0.25 sq m per site emptied monthly, plus 10 soil cores of 5 cm diameter to 10 cm depth germinated for 6 months
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
UK: none; landowner permission and SSSI consent for taking soil. US: none on private land; federal land needs a soil collection permit.
How to run it
  1. Set 10 to 20 funnel traps of known area on posts 30 cm above ground in a grid and note the area of each.
  2. Empty traps monthly into labelled bags, dry, sieve and count seeds by species under a lens.
  3. Take 10 soil cores of 5 cm diameter to 10 cm depth per site, keeping the litter and the 0 to 5 cm and 5 to 10 cm layers separate.
  4. Spread each core 1 cm thick over sterile compost in trays in a greenhouse, water daily and keep control trays of compost alone.
  5. Identify and remove every seedling as it becomes recognisable for 6 months, stir the soil at 3 months, and report seeds per square metre.
Kit list

The day one mistake: Not running control trays. Greenhouse compost and airborne seed produce seedlings that then get counted as the seed bank. Always run 3 trays of compost alone beside the samples and subtract what germinates in them.

Plants and vegetation

Tree regeneration plots

Count seedlings and saplings by height class in small plots to measure whether a wood is regenerating and whether deer browsing is stopping it. Follows the Forestry Commission and USFS Forest Inventory regeneration subplot method.

Answers
How many?, How is it spread across the area?, Is it here?
Effort
20 to 30 circular plots of 2 m radius on a grid, seedlings and saplings counted by height class, 1 visit per year in leaf
Season
Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: none; landowner permission. US: none on private land; National Forest research needs a special use permit.
How to run it
  1. Lay a grid at 50 m spacing across the wood and mark each plot centre with a peg and GPS.
  2. At each point count every woody plant under 1.3 m tall within a 2 m radius, by species and height class (under 20 cm, 20 to 50 cm, 50 to 130 cm).
  3. Count saplings over 1.3 m tall and under 7 cm diameter within a 4 m radius.
  4. Score browsing on the leading shoot of the nearest 10 saplings as browsed or not, and note deer dung and bark stripping.
  5. Record canopy cover, ground flora dominance and light, and repeat annually in July or August.
Kit list

The day one mistake: Counting only the healthy saplings and skipping the browsed stumps. The browsed ones are the evidence. Count every stem and score browsing separately so the deer effect is visible.

Plants and vegetation

Biomass clip plots

Cut all the vegetation inside a small quadrat, dry it and weigh it to give standing crop in grams per square metre. The direct measure of productivity behind grazing and restoration studies.

Answers
How many?, How is it spread across the area?
Effort
10 to 20 clip plots of 0.25 sq m per site, clipped to 1 cm above ground, dried 48 h at 60 C, 1 visit at peak standing crop
Season
Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: SSSI consent for destructive sampling; landowner permission. US: none on private land; federal land needs a research permit for any collection.
How to run it
  1. Place 10 to 20 quadrats of 0.25 sq m at random points and photograph each before clipping.
  2. Clip everything rooted inside the frame to 1 cm above the soil with shears, sorting into live, dead and litter, or by functional group if needed.
  3. Bag each fraction in paper with a label inside, and record fresh weight if you can.
  4. Dry at 60 C for 48 h or until constant weight and weigh to 0.1 g.
  5. Multiply by 4 for grams per square metre and note grazing exclusion, cutting date and rainfall.
Kit list

The day one mistake: Weighing fresh. Water content varies from 40 to 80 percent between mornings and afternoons, so fresh weights are not comparable. Dry to constant weight every time.

Fungi, lichens and bryophytes

Deadwood fungi fixed log surveys

Tag a set of fallen logs and record every fungal fruit body on them each year, with the log decay stage, so species turnover can be followed through decomposition. Follows the fixed log monitoring used in Swedish deadwood fungi studies and by UK fungus groups.

Answers
Is it here?, How is it spread across the area?, How many?
Effort
20 to 50 tagged logs over 10 cm diameter per site, whole surface examined for 10 minutes each, 2 visits in autumn and 1 in spring
Season
Apr, Aug, Sep, Oct, Nov (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: none; landowner permission and SSSI consent for collecting vouchers. US: none on private land; National Forests and parks need a collecting permit for vouchers.
How to run it
  1. Choose 20 to 50 logs over 10 cm diameter and 1 m long, tag each with a numbered disc and GPS, and record species, length, diameter and decay class 1 to 5.
  2. Search the whole log surface including the underside and bark crevices for 10 minutes with a lens and torch.
  3. Record every fruit body by species with an abundance class, and photograph anything you cannot name.
  4. Take a small voucher of unknown brackets and crusts only, in paper, noting the log number.
  5. Repeat in September, October and April each year, and note when a log moves to the next decay class.
Kit list
  • Hand lens 10x
  • Headlamp
  • Tree tags (Forestry Suppliers (US))
  • Diameter tape (Forestry Suppliers (US) or NHBS (UK))
  • Mushroom field guide (NHBS (UK) or a bookshop (US))

The day one mistake: Rolling the log to see underneath. That kills the fauna and changes the moisture of the whole community. Lie on the ground with a torch and mirror and leave the log where it is.

Marine benthos, reefs and shore

Seagrass transects and quadrats

Lay a tape across a seagrass bed and score percent cover, shoot density and canopy height in quadrats, following Seagrass Watch and the SeagrassNet protocol. Intertidal beds can be walked at low water and subtidal ones snorkelled.

Answers
How many?, How is it spread across the area?, Is it here?
Effort
3 transects of 50 m per site, 0.25 sq m quadrats every 5 m, 11 quadrats per transect, 1 visit per year at peak biomass
Season
Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
UK: none for observation; seagrass beds are mostly within MCZs or SACs and anchoring or pegging needs MMO consent and the site manager. US: none for observation; state seagrass protection laws restrict any disturbance, and federal permits apply in sanctuaries.
How to run it
  1. Set 3 parallel 50 m transects 25 m apart from the shallow edge and mark the start posts with GPS.
  2. At 0, 5, 10 and every 5 m to 50 m place a 0.25 sq m quadrat on the right of the tape and estimate percent cover of seagrass, algae and epiphytes using the Seagrass Watch photo guide.
  3. Count shoots in a 0.01 sq m corner of the quadrat, measure the 5 longest leaves and note the species.
  4. Photograph every quadrat from directly above with the label visible.
  5. Record sediment type, grazing marks, wasting disease lesions and water depth, and repeat in the same month each year.
Kit list

The day one mistake: Walking through the bed to place quadrats. Every step crushes rhizomes and the transect degrades year by year. Walk only along the tape line on the left side and place quadrats to the right.

Marine benthos, reefs and shore

Mangrove plots and transects

Run transects across the mangrove and measure every tree in fixed plots for species, diameter and height, with seedling counts in subplots. The Mangrove Action Project and CIFOR blue carbon protocol.

Answers
How many?, How is it spread across the area?, Is it here?
Effort
3 transects from seaward edge to landward edge, 10 by 10 m plots every 25 m, all trees over 2.5 cm diameter measured, 1 visit per year
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
UK: not applicable. US: Florida and other states protect mangroves and need a permit for any cutting or coring; federal refuges need a research permit. Elsewhere national forestry permits usually apply.
How to run it
  1. Check tides and set transects at low water from the seaward fringe to the landward limit with GPS at each plot corner.
  2. Mark 10 by 10 m plots every 25 m and tag every tree with a diameter over 2.5 cm at 1.3 m, or above the highest prop root for Rhizophora.
  3. Measure diameter and height and record species and condition (live, dead, cut).
  4. Count seedlings and saplings under 2.5 cm in a 2 by 2 m subplot in each corner.
  5. Take a 30 cm soil core for carbon where the study needs it, record crab burrow density and salinity, and repeat annually.
Kit list

The day one mistake: Measuring Rhizophora diameter at 1.3 m where the prop roots are. The reading is meaningless and cannot be repeated. Measure 30 cm above the highest prop root and write the height used.

Marine benthos, reefs and shore

Drop down video, towed camera and ROV seabed surveys

Lower a camera to the seabed on a frame or tow it slowly to record habitat and epifauna where divers cannot go. The JNCC and NOAA method for mapping reefs, biogenic habitats and impacts on the shelf.

Answers
How is it spread across the area?, Is it here?, How many?
Effort
20 to 50 stations per site, 1 to 3 minutes of video per drop, or 100 to 500 m tows at 0.5 knots, 1 survey per year in calm weather
Season
Apr, May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Funded project
Licence
UK: MMO marine licence is not needed for a camera drop but is for any towed gear that touches the bed in an MPA; ask the site manager. US: sanctuary and state permits for towed gear; none for a drop camera outside protected areas.
How to run it
  1. Plan stations on a grid or along a gradient in the GIS and check the weather window for under 1 m swell.
  2. Fit lasers 10 cm apart or a scale bar in the field of view and log GPS, depth and time on every drop.
  3. Lower the camera until the bed is in view, hold for 1 to 3 minutes or drift slowly, and record 3 stills.
  4. For tows keep the camera 0.5 to 1 m off the bed at 0.5 knots and note the start and end positions.
  5. Analyse video to EUNIS or CMECS habitat class with SACFOR abundance for conspicuous species, and archive the raw footage with the metadata.
Kit list

The day one mistake: Towing too fast. Above 1 knot the video is a blur and no species can be named. Hold the boat at 0.5 knots and let the camera hang, even if the survey takes twice as long.

Marine benthos, reefs and shore

Intertidal sediment cores for infauna

Push a core tube into mud or sand at low water and sieve the sediment to count the worms, bivalves and crustaceans that feed shorebirds and fish. The Environment Agency and NOAA benthic infauna method.

Answers
How many?, How is it spread across the area?, Is it here?
Effort
5 replicate cores of 10 cm diameter to 15 cm depth per station, 5 to 10 stations per shore, sieved at 0.5 mm, 1 visit per year
Season
Mar, Apr, May, Sep, Oct, Nov (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
UK: none for hand coring; SSSI consent and the site manager, and Crown Estate permission for large studies. US: state coastal permits and refuge research permits; none on private beaches.
How to run it
  1. Set stations down the shore from the strandline to low water on a spring tide and mark with GPS.
  2. At each station take 5 cores of 10 cm diameter to 15 cm depth 1 m apart and bag the sediment.
  3. Sieve each core through a 0.5 mm mesh at the shore with seawater and put the residue into a pot of 10 percent buffered formalin or 70 percent ethanol.
  4. Take a sixth core for particle size and organic content.
  5. Sort under a microscope in the lab, count and identify to family or species, and report per 0.1 sq m.
Kit list

The day one mistake: Sieving without keeping the water in the residue. Small polychaetes are lost through a dry sieve. Sieve gently under water in a tray and wash the residue into the pot with a squeeze bottle.

Marine benthos, reefs and shore

Strandline surveys

Walk the tide line and record the shells, egg cases, seaweeds, litter and dead animals cast up, following the Marine Conservation Society Beachwatch and the Shark Trust Great Eggcase Hunt methods. Cheap, year round and open to volunteers.

Answers
Is it here?, How is it spread across the area?, How many?
Effort
100 m of strandline searched for 30 minutes, monthly or after storms, 1 to 3 sections per beach
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: none; dead cetaceans, turtles and some birds must be reported rather than collected. US: none; do not touch marine mammals or sea turtles, which are protected even when dead, and report them to the stranding network.
How to run it
  1. Mark a 100 m section of beach with GPS at each end and survey on a falling tide after the top of the tide.
  2. Walk the strandline slowly for 30 minutes listing every type of natural item and count egg cases, shells and stranded animals.
  3. For litter follow the Beachwatch categories and count items per 100 m.
  4. Photograph every egg case and unknown item on a scale card and soak egg cases before identifying.
  5. Report strandings of turtles, cetaceans and seals to the strandings network and log the rest with the recording scheme.
Kit list

The day one mistake: Surveying a beach that was cleaned that morning. Many beaches are raked at dawn in summer and the strandline is gone. Ask the council when cleaning happens and survey before it.

Marine benthos, reefs and shore

Reef invertebrate belt transects (Reef Check)

Swim a 5 m wide belt along a fixed 100 m line and count the indicator invertebrates and impacts, then use a line intercept for substrate. The Reef Check protocol used worldwide for reef health.

Answers
How many?, Is it here?, How is it spread across the area?
Effort
4 segments of 20 m on a 100 m line, 5 m wide belt, 2 depths (2 to 6 m and 6 to 12 m), 1 survey per year
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
UK: none for observation; MPA site managers ask for notification. US: sanctuary permits for placing any fixed marker; none for observation. Elsewhere the national park or fisheries office usually issues a research permit.
How to run it
  1. Complete Reef Check training and lay the 100 m transect along a depth contour at 2 to 6 m, marking the start with GPS.
  2. Swim the 4 segments of 20 m (0 to 20, 25 to 45, 50 to 70, 75 to 95 m) counting the indicator species within 2.5 m each side: urchins, sea cucumbers, crown of thorns, giant clams, lobsters, banded shrimp.
  3. Search under ledges with a torch and count anemones and their shrimps once per segment.
  4. Record impacts per segment: bleaching, disease, trash, anchor damage, fishing nets.
  5. Repeat at the 6 to 12 m depth and enter data on the Reef Check forms the same day.
Kit list

The day one mistake: Counting outside the belt. A lobster seen 4 m off the line is not on the transect. Hold a 2.5 m length of line in one hand and use it to test every doubtful animal.

Marine benthos, reefs and shore

Seasearch style subtidal dive surveys (SACFOR)

Dive a site and describe every habitat met with its depth, substrate and conspicuous species scored on the SACFOR scale. The Seasearch method run by the Marine Conservation Society and adopted by JNCC for MPA condition reporting.

Answers
Is it here?, How is it spread across the area?, How many?
Effort
1 dive of 30 to 45 minutes per site, habitat and species recorded in SACFOR abundance classes, 1 to 3 dives per site per year
Season
Apr, May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
UK: none for observation; MCZ and SAC byelaws may restrict anchoring, and the harbour authority should be told. US: none for recreational observation; sanctuary and state park research permits if you collect or mark anything.
How to run it
  1. Complete the Seasearch Observer or Surveyor course and plan the dive with buddy, depth limit and GPS of the entry point.
  2. Descend and note the depth, substrate, slope and visibility at the start, then follow a slow route across the habitats.
  3. For each habitat record its depth range, substrate and up to 10 conspicuous species with a SACFOR class (Superabundant, Abundant, Common, Frequent, Occasional, Rare).
  4. Photograph each habitat and every species you cannot name for later ID.
  5. Fill in the Seasearch form the same day with sketch, depth profile and impacts seen, and send it to the coordinator.
Kit list

The day one mistake: Listing species without a habitat. A record of 30 species with one depth is not a Seasearch form. Describe each habitat as its own record with its depth band and give the species to it.

Habitat and environment

Habitat condition assessment

Walk a W across each habitat parcel and score indicator species, sward structure, bare ground, scrub and negative indicators at 20 stops against a target. The Common Standards Monitoring approach used by JNCC and the Biodiversity Metric habitat condition sheets.

Answers
How is it spread across the area?, How many?, Is it here?
Effort
20 stops per habitat parcel on a W walk, 1 sq m assessed at each stop, whole site in 1 visit per year
Season
May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: none; landowner permission and SSSI notification. US: none on private land; federal land needs a research permit for repeated access.
How to run it
  1. Map the site into parcels of one habitat type and pick the condition sheet for that habitat from the guidance.
  2. Walk a W shaped route across the parcel and stop 20 times at equal intervals.
  3. At each stop assess 1 sq m: count positive indicator species, note negative indicators, sward height, bare ground and litter.
  4. Across the whole parcel record scrub cover, invasive species, damage and management as percent classes.
  5. Compare each attribute to its target and record pass or fail, then give the parcel an overall condition of good, moderate or poor.
Kit list
  • Quadrat 1 m (NHBS (UK), Forestry Suppliers (US), or make from PVC pipe)
  • Handheld GPS
  • Wildflower field guide (NHBS (UK) or a bookshop (US))
  • Clipboard
  • Drop disc (make from a 30 cm plastic disc and a graduated rod, or NHBS (UK))

The day one mistake: Stopping where the flowers are. The 20 stops must fall at fixed paces on the W whether the ground is rich or poor, or every parcel scores as good. Count paces and stop on the number.

Habitat and environment

Water quality field measurements (sonde, turbidity)

Take temperature, dissolved oxygen, pH, conductivity and turbidity at a fixed point with a sonde or handheld meters so the biology has its chemistry. The EPA volunteer monitoring and Environment Agency spot sampling method.

Answers
How is it spread across the area?, Is it here?
Effort
1 station per site sampled monthly, or spot readings at every biological sample, readings at 10 cm below surface in flowing water
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
UK: none; landowner permission and EA consent for permanent installations. US: none; state volunteer monitoring programmes may require QA training to accept data.
How to run it
  1. Calibrate the meters the same morning with fresh standards and record the calibration in the log.
  2. At the station stand downstream and hold the probe 10 cm below the surface in flowing water away from the bank for 2 minutes until it settles.
  3. Read and record each parameter, then take a grab sample in a clean bottle for turbidity, measured in a tube or nephelometer.
  4. Note flow, weather and any discharge or colour, and photograph the station.
  5. Rinse probes with distilled water, cap them wet, Check Clean Dry boots and waders, and return to the same station at the same time of day each month.
Kit list

The day one mistake: Reading dissolved oxygen as soon as the probe goes in. It takes 2 minutes to settle and the first reading is high. Hold it still, wait for the number to stop moving, then write it down.

Habitat and environment

River Habitat Survey and MoRPh

Record the physical structure of a river reach, its banks, channel features, vegetation and modifications, following the Environment Agency River Habitat Survey or the citizen science MoRPh method of the Modular River Survey.

Answers
How is it spread across the area?, Is it here?
Effort
RHS: 500 m reach with 10 spot checks at 50 m intervals plus a sweep up; MoRPh: 10 to 40 m module, 1 visit in low flow
Season
May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: none; landowner permission and accredited training to submit RHS. US: the equivalent is the EPA physical habitat protocol, no permit for observation.
How to run it
  1. Complete the RHS or MoRPh training and choose a 500 m reach (RHS) or a module of 10 times the channel width (MoRPh).
  2. At each of the 10 spot checks 50 m apart record bank material, bank modifications, channel substrate, flow type and channel vegetation on both banks.
  3. Between spot checks note land use, bankside trees, features such as bars, riffles, pools and eroding cliffs, and every artificial structure.
  4. Photograph each spot check, measure bankfull width and depth at one representative point, and Check Clean Dry waders before the next reach.
  5. Enter data in the RHS or Modular River Survey database, which returns the Habitat Quality and Habitat Modification scores.
Kit list

The day one mistake: Estimating the spot check spacing by eye. Spot checks that drift to 30 m or 80 m apart break the comparability with 25,000 other reaches. Pace or tape the 50 m every time.

Habitat and environment

Peatland condition and peat depth probing

Push a rod through the peat to the mineral base to map depth, and score the surface for bare peat, erosion, Sphagnum cover and drainage. The IUCN UK Peatland Programme and Peatland Code condition categories.

Answers
How is it spread across the area?, How many?, Is it here?
Effort
Depth probes on a 100 m grid or every 50 m on transects, condition scored in 2 by 2 m quadrats at each probe, 1 visit
Season
May, Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: SSSI consent for probing and landowner permission. US: none on private land; federal wetlands need a research permit and state wetland rules may apply.
How to run it
  1. Lay a 100 m grid over the site with GPS and load the points into the handheld.
  2. At each point push the probe until it stops on mineral ground, add extension rods, and record depth to the nearest 10 cm.
  3. Score a 2 by 2 m quadrat at the point: Sphagnum cover, bare peat, cotton grass, heather, erosion gullies and hagging, and any drain within 10 m.
  4. Assign the point to the Peatland Code category (near natural, modified, drained, eroded) and photograph it.
  5. Map peat depth and condition in the GIS and note the water table state and grazing.
Kit list
  • Peat probe (make from 1 m threaded steel rods with a T handle, or Van Walt (UK) or Forestry Suppliers (US))
  • Handheld GPS
  • Quadrat 2 by 2 m (make from four 2 m canes and string, or NHBS (UK))
  • Rugged digital camera
  • Mosses field guide (FSC or NHBS (UK); a bookshop (US))

The day one mistake: Stopping the probe on a buried tree stump and calling it the base. Bog wood gives a sharp stop at 1 m where the peat goes on to 4 m. If the stop feels sharp and shallow, probe again 1 m away.

Habitat and environment

Water table dipwells and soil moisture

Sink perforated tubes into the ground and measure the depth to water in each on a regular cycle to track the water table that drives wetland plants and peat. The standard for restoration monitoring under the Peatland Code and ditch blocking projects.

Answers
How is it spread across the area?, Is it here?
Effort
5 to 10 dipwells per site on a transect from drain to centre, read weekly or fortnightly, or logged hourly with a pressure logger
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: SSSI consent and landowner permission for installing wells. US: none on private land; state wetland rules and federal permits for wells on public land.
How to run it
  1. Cut a 1 m length of 32 mm perforated pipe with a cap, and install 5 to 10 along a transect at 5, 10, 20, 40 and 80 m from a drain.
  2. Auger a hole, push the pipe in with 10 cm proud of the surface, and measure the height of the top above ground.
  3. Read weekly with a dip tape or blow tube, recording depth from the pipe top and correcting to ground level.
  4. For continuous data hang a pressure logger at the bottom of one well and a barometric logger nearby, downloading monthly.
  5. Take a soil moisture reading with a probe at 3 points around each well on every visit and note rainfall in the previous 7 days.
Kit list

The day one mistake: Reading the depth from the top of the pipe and never measuring how far the pipe sticks out. Pipes heave and sink, and the record becomes noise. Measure pipe height above ground on every visit and correct every reading.

Habitat and environment

Soil sampling for ecology (pH, organic matter, bulk density)

Take soil cores for pH, organic matter and bulk density so vegetation and invertebrate data can be explained by soil. Follows the standard methods of the UK Countryside Survey and USDA NRCS soil sampling guidance.

Answers
How is it spread across the area?, Is it here?
Effort
5 to 10 sampling points per parcel, each a composite of 5 cores to 10 cm, plus 3 bulk density rings per parcel, 1 visit
Season
Mar, Apr, May, Sep, Oct, Nov (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: SSSI consent for digging and landowner permission; scheduled monuments prohibit digging without Historic England consent. US: none on private land; federal land needs a soil collection permit.
How to run it
  1. Choose 5 to 10 points per parcel at random and at each take 5 cores to 10 cm in a 2 m circle, mix them in a bag as one composite.
  2. Push a 100 cc steel ring into the soil at 3 points, dig it out level, trim, bag and later dry at 105 C for 24 h to get bulk density.
  3. At home air dry the composite samples, sieve to 2 mm and measure pH in a 1 to 2.5 soil to water mix with a calibrated meter.
  4. Measure organic matter by loss on ignition at 550 C for 4 h, or send to a lab for carbon.
  5. Record soil texture by hand, colour, depth to stones or bedrock, and drainage class at each point.
Kit list

The day one mistake: Sampling one pit per field. Soil pH can change by 2 units across a field and one pit is a guess. Composite 5 cores at each of several points so the mean is real.

Habitat and environment

Canopy cover and light (densiometer, hemispherical photos)

Estimate canopy cover with a spherical densiometer or a fisheye photo so light reaching the ground can be linked to regeneration, ground flora and butterflies. The USFS Forest Inventory and the UK Woodland Survey Handbook use this.

Answers
How is it spread across the area?, How many?
Effort
4 densiometer readings per plot facing N, E, S and W at 1.3 m, or 1 hemispherical photo per plot at 1 m, 1 visit in full leaf
Season
Jun, Jul, Aug, Sep (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: none; landowner permission. US: none; National Forests need a permit for research plots.
How to run it
  1. Stand at the plot centre and hold the densiometer level at 1.3 m, 30 cm from your body, facing north.
  2. Count the dots or squares covered by canopy in the mirror grid, and repeat facing east, south and west; average the 4 and convert to percent.
  3. For photos set a camera with a fisheye lens level on a tripod at 1 m, top of image to north, and shoot in even overcast light or at dawn.
  4. Process photos in free software such as ImageJ or Gap Light Analyzer for canopy openness and leaf area index.
  5. Record tree species and height at each plot and repeat in the same month each year.
Kit list

The day one mistake: Taking photos in full sun. Sun flare and bright leaves read as sky and the openness value is far too high. Shoot in uniform overcast or in the half hour before sunrise.

Habitat and environment

Microclimate loggers

Leave small temperature and humidity loggers in shields at ground or canopy level to measure the microclimate that animals and plants actually feel. The SoilTemp and TMS network standards.

Answers
How is it spread across the area?, Is it here?
Effort
5 to 20 loggers per site in a gradient or paired design, 30 to 60 minute logging interval, downloaded every 3 to 6 months
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Some kit (up to $1,000)
Licence
UK: none; landowner and SSSI consent for stakes. US: none on private land; federal land needs a permit for installations.
How to run it
  1. Decide the question first and place loggers in pairs or along the gradient it needs, for example open versus shaded or slope top to bottom.
  2. Mount each logger in a radiation shield at a fixed height (5 cm, 15 cm, 1.5 m) on a stake, away from direct sun on the sensor.
  3. Set a 30 to 60 minute interval, synchronise the clocks, and log GPS, height, aspect and canopy cover for each unit.
  4. Download every 3 to 6 months, check for gaps and drift against a reference, and replace batteries.
  5. Archive the raw files with the metadata and report daily minimum, mean and maximum by site.
Kit list

The day one mistake: Hanging a bare logger on a tree. Sun on the case gives 10 C errors at midday and the data is unusable. Put every sensor in a shield and note its height and aspect.

Habitat and environment

Deadwood line intersect surveys

Walk fixed lines and measure the diameter of every dead log that crosses them to estimate deadwood volume per hectare, and count snags. The Van Wagner line intersect method used by the USFS and Forestry Commission.

Answers
How many?, How is it spread across the area?
Effort
3 lines of 50 m per plot in a triangle, every piece over 7.5 cm diameter crossing the line measured, standing dead trees counted in a 20 m radius, 1 visit
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: none; landowner permission. US: none on private land; National Forests need a permit for marked plots.
How to run it
  1. At the plot centre lay 3 tapes of 50 m in a triangle at bearings 0, 120 and 240 degrees.
  2. Walk each line and at every log over 7.5 cm diameter that crosses it measure the diameter at the crossing point and note species and decay class 1 to 5.
  3. Count and measure standing dead trees over 10 cm diameter within a 20 m radius of the centre.
  4. Note fine woody debris under 7.5 cm as a count on the first 10 m of each line.
  5. Calculate volume as pi squared times the sum of squared diameters divided by 8 times the line length, and report cubic metres per hectare.
Kit list
  • Tape measure 50 m
  • Diameter tape (Forestry Suppliers (US) or NHBS (UK))
  • Forestry calipers (Haglof via Forestry Suppliers (US) or NHBS (UK))
  • Compass
  • Decay class card (print from the Forestry Commission or USFS guidance)

The day one mistake: Measuring the widest diameter of the log. The formula wants the diameter at the exact point where the line crosses it. Hold the tape on the line and measure there.

Habitat and environment

Fixed point photography

Photograph the same view from a marked post at the same time each year to show habitat change that numbers miss. Used in every long term management plan and by the Natural England monitoring guidance.

Answers
How is it spread across the area?, What is it doing?
Effort
5 to 20 posts per site, 1 to 4 photos per post at fixed bearings, repeated in the same month every year or every season
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: none; landowner and SSSI consent for posts. US: none; federal land needs approval for permanent markers.
How to run it
  1. Choose views that show the management question, install a post or use a permanent feature, and record GPS, bearing and camera height for each.
  2. Photograph at 1.5 m on a tripod with a 35 mm equivalent lens, using the previous photo on a phone to match the frame.
  3. Take the photo in the same month and similar light each year, and include a scale bar or the post in the foreground.
  4. Log camera, lens, time, weather and any changes to the post in the register.
  5. File photos by post and date with a copy in a shared archive, and print pairs for the annual report.
Kit list

The day one mistake: Zooming to make the shot look good. A different focal length cannot be compared with last year. Fix the lens, the height and the bearing and let the picture be dull.

Habitat and environment

Sward height and structure (drop disc)

Drop a light disc down a graduated rod and read where it rests on the grass to measure sward height and structure for grazing management and bird and invertebrate habitat. The RSPB and Natural England sward measurement method.

Answers
How many?, How is it spread across the area?
Effort
20 to 50 drops per field on a W walk, read to 0.5 cm, monthly through the grazing season
Season
Apr, May, Jun, Jul, Aug, Sep, Oct (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: none; landowner permission. US: none; grazing allotments on public land need a permit for monitoring plots.
How to run it
  1. Make or buy a disc of 30 cm diameter and about 200 g on a rod marked in 0.5 cm.
  2. Walk a W across the field and drop the disc from 30 cm above the sward at 20 to 50 evenly spaced points.
  3. Read the height where the disc rests to 0.5 cm and note whether the vegetation is grass, herb, rush or bare.
  4. Also record the sward as a structure class (short, tussocky, tall) and any litter mat at each point.
  5. Report the mean, the percent of drops under 5 cm and over 15 cm, and repeat monthly through the grazing season.
Kit list

The day one mistake: Pressing the disc down or dropping from head height. Both change the reading by centimetres and the months cannot be compared. Drop from 30 cm every time and let it settle.

Habitat and environment

Citizen science recording (iNaturalist, eBird)

Record what you see with the phone apps that feed the global databases, so casual records become usable data. eBird wants complete checklists with effort, and iNaturalist wants a photo or sound with a location.

Answers
Is it here?, How is it spread across the area?, How many?
Effort
1 complete checklist or observation set per visit, any length, submitted the same day, as often as you go out
Season
All year (northern hemisphere; shift 6 months in the south)
Budget
Almost nothing (under $100)
Licence
UK: none; do not enter private land and consider obscuring locations of rare species. US: none; refuges and parks welcome records but photographing listed species at nests needs a permit.
How to run it
  1. Install eBird and iNaturalist, set the location accuracy to on, and read the app guidance on complete checklists and obscured locations.
  2. For birds start a checklist at the start of the walk, record every species and count, and stop it at the end so the app has distance and time.
  3. For everything else photograph the organism with a clear view of the key features and one wider shot of the habitat.
  4. Add a species name if you know it or leave the app to suggest, and mark captive or planted organisms as such.
  5. Submit the same day, respond to identifications from other users, and download your own data each year as a backup.
Kit list

The day one mistake: Uploading a photo of a rare orchid with an exact grid reference. Collectors search the databases. Set the geoprivacy to obscured for anything protected or vulnerable before you press submit.

Methods follow standard published protocols (national monitoring schemes, Sutherland’s Ecological Census Techniques, agency guidance). Always follow your project’s own protocol and licence conditions where they differ. Kit links to Amazon are affiliate links. Tool by Erzsebet Frey, Simply Ecologist.

If you would rather find animals than survey them, the same field logic sits behind this guide to wildlife in the USA, park by park.

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